2025

715. Sohn, Y.J., Hwang, S.-Y., Lee, H., Jeon, S., Park, J.Y., Kim, J., Kim, D., Jeong, K.J., Lee, S.Y., Joo, J.C., Park, J.-B., and Park, S.J. "Metabolic engineering of Corynebacterium glutamicum for high-level production of 1,5-pentanediol, a C5 diol platform chemical", Adv. Sci. in press.

714. Kim, G.-M., Choi, Y., Choi, K.R., Lee, I., Kim, J., Lee, B., Lee, S.Y. and Lee, D.C., "In vivo synthesis of semiconductor nanoparticles in Azotobacter vinelandii for light-driven ammonia production", Nanoscale (2025) https://doi.org/10.1039/d4nr02177k

713. Lee, J., Yu, H.E., and Lee, S.Y. "Metabolic engineering of microorganisms for carbon dioxide utilization", Curr. Opin. Biotechnol., 91: 103244 (2025.2) https://doi.org/10.1016/j.copbio.2024.103244

712. Cho, S., Jung, S.Y., Eun, H., and Lee, S.Y. "Precision fermentation for producing food ingredients", Curr. Opin. Food Sci., 61:101242 (2025.2) https://doi.org/10.1016/j.cofs.2024.101242

2024

711. Lee, Y., Kang, M., Jang, W.D., Choi, S.Y., Yang, J.E., and Lee, S.Y. "Microbial production of an aromatic homopolyester", Trends Biotechnol., 42(11): 1453-1478 (2024.11)  https://doi.org/10.1016/j.tibtech.2024.06.001

710. Cho, J.S., Luo, Z.W., Moon, C.W., Prabowo, C.P.S., and Lee, S.Y. "Metabolic engineering of Corynebacterium glutamicum for the production of pyrone and pyridine dicarboxylic acids", Proc. Nat. Acad. Sci. (PNAS), 121 (45): e2415213121 (2024), https://doi.org/10.1073/pnas.2415213121

709. Zou, X., Mo, Z., Wang, L., Chen, S.and Lee, S.Y., "Overcoming Bacteriophage Contamination in Bioprocessing: Strategies and Applications", small methods, 2400932, https://doi.org/10.1002/smtd.202400932 (2024.10), https://doi.org/10.1002/smtd.202400932  

708. Kim, J.Y., Lee, J.A., Ahn, J.H., and Lee, S.Y. "High-level succinic acid production by overexpressing a magnesium transporter in Mannheimia succiniciproducens", Proc. Nat. Acad. Sci. (PNAS), 121(37): e2407455121, https://doi.org/10.1073/pnas.2407455121 (2024.9), https://doi.org/10.1073/pnas.2407455121  

707. Wang, F., Jiang, X., Liu, Y., Zhang, G., Zhang, Y., Jin, Y., Shi, S., Men, X., Liu, L., Wang, L., Liao, W., Chen, X., Chen, G., Liu, H., Ahmad, M., Fu, C., Wang, Q., Zhang, H., and Lee, S.Y. "Tobacco as a promising crop for low-carbon biorefinery", The Innovation, 5(5): 100687 (2024.9), https://doi.org/10.1016/j.xinn.2024.100687 

706. Lee, S.Y. and Kim, G.B. "Understanding the metabolism of infants using whole-body metabolic models", Cell Metabolism 36(8): 1641-1643 (2024.8), https://doi.org/10.1016/j.cmet.2024.07.001

705. Lee, S.Y. "Fungible and non-fungible technologies in biomanufacturing scale-up", Nature Chemical Engineering, 1:(7) 442-443, https://doi.org/10.1038/s44286-024-00093-7 (2024.7), https://doi.org/10.1038/s44286-024-00093-7

704. Choi, K.R., Ahn, D.-H., Jung, S.Y., Lee, Y.H. & Lee, S.Y., "Microbial lysates repurposed as liquid egg substitutes", npj Science of Food, 8:35 (2024.6), https://doi.org/10.1038/s41538-024-00281-y

703. Timmis, K., Hallsworth, J.E., McGenity, T.J., Armstrong, R., Colom, M.F., Karahan, Z.C., Chavarría, M., Bernal, P., Boyd, E.S., Ramos, J.L., Kaltenpoth, M., Pruzzo, C., Clarke, G., López-Garcia, P., Yakimov, M.M., Perlmutter, J., Greening, C., Eloe-Fadrosh, E., Verstraete, W., Nunes, O.C., Kotsyurbenko, O., Nikel, P.I., Scavone, P., Häggblom, M.M., Rob Lavigne, R., Roux, F.L., Timmis, J.K., Parro, V., Michán, C., García, J.L., Casadevall, A., Payne, S.M., Frey, J., Koren, O., Prosser, J.I., Lahti, L., Rup Lal, R., Anand, S., Sood, U., Offre, P., Bryce, C.C., Mswaka, A.Y., Jores, J., Kaçar, B., Blank, L.M., Maaßen, N., Pope, P.B., Banciu, H.L., Armitage, J., Lee, S.Y., Wang, F., Makhalanyane, T.P., Gilbert, J.A., Wood, T.K., Vasiljevic, B., Soberón, M., Udaondo, Z., Rojo, F., Tamang, J.P., Giraud, T., Ropars, J., Ezeji, T., Müller, V., Danbara, H., Averhoff, B., Sessitsch, A., Partida-Martínez, L.P., Huang, W., Molin, S., Junier, P., Amils, R., Wu, X.-L., Ron, E., Erten, H., Martinis, E.C.P., Rapoport, A., Öpik, M., Pokatong, W.D.R., Stairs, C., Amoozegar, M.A., Serna, J.G., "A concept for international societally relevant microbiology education and microbiology knowledge promulgation in society" Microb. Biotechnol., 17(5): e14456, https://doi.org/10.1111/1751-7915.14456 (2024.5), https://doi.org/10.1111/1751-7915.14456

702. Li, Y., Yin, D., Lee, S.Y. and Lv, Y., "Engineered polymer nanoparticles as artificial chaperones facilitating the selective refolding of denatured enzymes", Proc. Nat. Acad. Sci. (PNAS), PNAS 2024 Vol. 121 No. 19 e2403049121, https://doi.org/10.1073/pnas.2403049121 (2024.5), https://doi.org/10.1073/pnas.2403049121

701. Choi, K.R., Jung, S.Y., and Lee, S.Y. "From sustainable feedstocks to microbial foods", Nature Microbiol., 9: 1167-1175, https://doi.org/10.1038/s41564-024-01671-4 (2024.5), https://doi.org/10.1038/s41564-024-01671-4

700. Lee, G., Lee, S.M., Lee, S., Jeong, C.W., Song, H., Lee, S.Y., Yun, H., Koh, Y. & Kim, H.U. "Prediction of metabolites associated with somatic mutations in cancers by using genome-scale metabolic models and mutation data" Genome Biol., 25(66) https://doi.org/10.1186/s13059-024-03208-8 (2024.3), https://doi.org/10.1186/s13059-024-03208-8

699. Eun, H. and Lee, S.Y. "Metabolic engineering and fermentation of microorganisms for carotenoids production", Curr. Opin. Biotechnol., 87:103104 (2024.6), https://doi.org/10.1016/j.copbio.2024.103104

698. Choi, K.R., Luo, Z.W., Kim, G.B., Xu, H., Lee, S.Y. "A microbial process for the production of benzyl acetate" Nature Chem. Eng., 1: 216-228, https://doi.org/10.1038/s44286-023-00022-0 (2024.3), https://doi.org/10.1038/s44286-023-00022-0

697. Torrente-Murciano, L., Dunn, J.B., Christofides, P.D., Keasling, J.D., Glotzer, S.C., Lee, S.Y., Van Geem, K.M, Tom, J. and He, G. "The forefront of chemical engineering research", Nature Chem. Eng. 1: 18-27 (2024.1), https://doi.org/10.1038/s44286-023-00017-x

696. Lee, J.H., Lee, S.-R., Lee, S.Y. and Lee, P.C. "Complete microbial synthesis of crocetin and crocins from glycerol in Escherichia coli", Microb. Cell Fact. 23(10) (2024.1), https://doi.org/10.1186/s12934-023-02287-9

695. Zhou, H., Eun, H. & Lee, S.Y. "Systems metabolic engineering for the production of pharmaceutical natural products", Curr. Opin. Sys. Biol., 37:100491 (2024.3), https://doi.org/10.1016/j.coisb.2023.100491 

2023

694. Lee, J.A., Kim, J.Y., Ahn, J.H., Ahn, Y.-J., and Lee, S.Y. "Current advancements in the bio-based production of polyamides", Trends Chem. 5(12): 873-891 (2023.12), https://doi.org/10.1016/j.trechm.2023.10.001

693. Choi, S.Y., Lee, Y., Yu, H.E., Cho, I.J., Kang, M. & Lee, S.Y. "Sustainable production and degradation of plastics using microbes", Nature Microbiol., 8: 2253-2276 (2023. 11), https://doi.org/10.1038/s41564-023-01529-1

692. Kim, W.J., Lee, Y., Kim, H.U., Ryu, J.Y., Yang, J.E., and Lee, S.Y. "Genome-wide identification of overexpression and downregulation gene targets based on the sum of covariances of the outgoing reaction fluxes", Cell Systems, 14(15): 990-1001.e5 (2023.11), https://doi.org/10.1016/j.cels.2023.10.005

691. Schimunek, J., Seidl, P., Elez, K., Hempel, T., Le, T., Noe, F., Olsson, S., Raich, L., Winter, R., Gokcan, H., Gusev, F., Gutkin, E.M., Isayev, O., Kurnikova, M.G., Narangoda, C.H., Zubatyuk, R., Bosko, I.P., Furs, K.V., Karpenko, A.D., Kornoushenko, Y.V., Shuldau, M., Yushkevich, A., Benabderrahmane, M.B., Bousquet-Melou, P., Bureau, R., Charton, B. Cirou, B.C., Gil, G., Allen, W.J., Sirimulla, S., Watowich, S., Antonopoulos, N.A., Epitropakis, N.E., Krasoulis, A.K., Pitsikalis, V.P., Theodorakis, S.T., Kozlovskii, I., Maliutin, A., Medvedev, A., Popov, P., Zaretckii, M., Eghbal-zadeh, H., Halmich, C., Hochreiter, S., Mayr, A., Ruch, P., Widrich, M., Berenger, F., Kumar, A., Yamanishi, Y., Zhang, K.Y.J., Bengio, E., Bengio, Y., Jain, M.J., Korablyov, M., Liu, C.-H., Marcou, G., Glaab, E., Barnsley, K., Iyengar, S.M., Ondrechen, M.J., Haupt, V. J., Kaiser, F., Schroeder, M., Pugliese, L., Albani, S., Athanasiou, C., Beccari, A., Carloni, P., D'Arrigo, G., Gianquinto, E., Goßen, J., Hanke, A., Joseph, B.P., Kokh, D.B., Kovachka, Sandra., Manelfi, C., Mukherjee, G., Muñiz-Chicharro, A., Musiani, F., Nunes-Alves, A., Paiardi, G., Rossetti, G., Sadiq, S.K., Spyrakis, F., Talarico, C., Tsengenes, A., Wade, R.C., Copeland, C., Gaiser, J., Olson, D.R., Roy, A., Venkatraman, V., Wheeler, T.J., Arthanari, H., Blaschitz, K., Cespugli, M., Durmaz, V., Fackeldey, K., Fischer, P.D., Gorgulla, C., Gruber, C., Gruber, K., Hetmann, M., Kinney, J.E., Das, K.M.P., Pandita, S., Singh, A., Steinkellner, G., Tesseyre, G., Wagner, Gerhard., Wang, Z.-F., Yust, R.J., Druzhilovskiy, D.S., Filimonov, D.A., Pogodin, P.V., Poroikov, V., Rudik, A.V., Stolbov, L.A., Veselovsky, A.V., Rosa, M.D., Simone, G.D., Maria R. Gulotta, M.R., Lombino, J., Mekni, N., Perricone, U., Casini, A., Embree, A., Gordon, D.B., Lei, D., Pratt, K., Voigt, C.A., Chen, K.-Y., Jacob, Y., Krischuns, T., Lafaye, P., Zettor, A., Rodríguez, M.L., White, K.M., Fearon, D., Delft, F.V., Walsh, M.A., Horvath, D., Brooks III, C.L., Falsafi, B., Ford, B., García-Sastre, A., Lee, S.Y., Naffakh, N., Varnek, A., Klambauer, G., Hermans, T.M. "A community effort in SARS-CoV-2 drug discovery", Molecular Informatics, 42: e202300262 (2023), https://doi.org/10.1002/minf.202300262

690. Kim, G.B., Kim, J.Y., Lee, J.A., Norsigian, C.J., Palsson, B.O. & Lee, S.Y. "Functional annotation of enzyme-encoding genes using deep learning with transformer layers", Nature Communications, 14:7370, DOI: 10.1038/s41467-023-43216-z (2023.11), https://doi.org/10.1038/s41467-023-43216-z

689. Chung, H., Kim, J., Lee, Y.J., Choi, K.R., Jeong, K.J., Kim, G.-J., and Lee, S.Y. "Enhanced production of difficult-to-express proteins through knocking down rnpA gene expression", Biotechnol. J., 18(10): 2200641 (2023.10), https://doi.org/10.1002/biot.202200641

688. Gu, L., Xiao, X., Lee, S.Y., Lai, B., Solem, C., "Superior anodic electro-fermentation by enhancing capacity for extracellular electron transfer", Bioresource Technol., 389: 129813 (2023.12), https://doi.org/10.1016/j.biortech.2023.129813

687. Han, T., Nazarbekov, A., Zou, X., and Lee, S.Y. "Recent advances in systems metabolic engineering", Curr. Opin. Biotechnol., 84:103004 (2023.12), https://doi.org/10.1016/j.copbio.2023.103004

686. Ryu, G., Kim, G.B., Yu, T., and Lee, S.Y. "Deep learning for metabolic pathway design", Metab. Eng., 80: 130-141 (2023.11), https://doi.org/10.1016/j.ymben.2023.09.012

685. Liu, L., Li, J., Gai, Y., Tian, Z., Wang, Y., Wang, T., Liu, P., Yuan, Q., Ma, H., Lee S.Y. & Zhang, D. "Protein engineering and iterative multimodule optimization for vitamin B6 production in Escherichia coli", Nature Comm., 14:5304, DOI: 10.1038/s41467-023-40928-0 (2023), https://doi.org/10.1038/s41467-023-40928-0

684. Kang, Q., Fang, H., Xiangm, M., Xiao, K., Jiang, P., You, C., Lee, S.Y., Zhang, D. "A synthetic cell-free 36-enzyme reaction system for vitamin B12 production", Nature Comm., 14: 5177, DOI: 10.1038/s41467-023-40932-4 (2023), https://doi.org/10.1038/s41467-023-40932-4

683. Whitford, C.M., Gren, T., Palazzotto, E., Lee, S.Y., Tong, Y., and Weber, T. "Systems analysis of highly multiplexed CRISPR-base editing in Streptomycetes", ACS Syn. Biol., 12(8): 2353–2366 (2023.8), https://doi.org/10.1021/acssynbio.3c00188

682. Han, T. and Lee, S.Y. "Metabolic engineering of Corynebacterium glutamicum for the production of valerolactam, a nylon-5 monomer", Metab. Eng., 79: 78-85 (2023.9), https://doi.org/10.1016/j.ymben.2023.07.002

681. Choi, K.R., Lee, S.Y., "Systems metabolic engineering of microorganisms for food and cosmetics production", Nature Reviews Bioeng., DOI: 10.1038/s44222-023-00076-y (2023.6), https://doi.org/10.1038/s44222-023-00076-y

680. Kim, J.Y., Lee, J.A., and Lee, S.Y. "Biobased Production of Succinic Acid and Its Derivatives Using Metabolically Engineered Microorganisms ", Ind. Biotechnol., 19(3): 125-137 (2023.6), https://doi.org/10.1089/ind.2023.0001

679. Gu, L., Xiao, X., Zhao, G., Kempen, P., Zhao, S., Liu, J., Lee, S.Y., and Solem, C. "Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer", Microb. Biotechnol., 16(6): 1277-1292 (2023.6), https://doi.org/10.1111/1751-7915.14229

678. Lee, J.A., Kim, H.U., Na, J.-G., Ko, Y.-S., Cho, J.S., and Lee, S.Y. "Factors Affecting the Competitiveness of Bacterial Fermentation", Trends Biotechnol., 41(6): 798-816 (2023.6), https://doi.org/10.1016/j.tibtech.2022.10.005

677. Cho, J.S., Yang, D., Prabowo, C.P.S., Ghiffary, M.R., Han, T.H., Choi, K.R., Moon, C.W., Zhou, H., Ryu, J.Y., Kim, H.U., and Lee, S.Y., "Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs", Nature Comm., 12: 173, DOI: 10.1038/s41467-023-38119-y (2023.4), https://doi.org/10.1038/s41467-023-38119-y

676. Jang, Y.-S., Kim, W.J., Im, J.A., Palaniswamy, S., Yao, Z., Lee, H.L., Yoon, Y.R., Seong, H.J., Papoutsakis, E.T., and Lee, S.Y. "Efforts to install a heterologous Wood-Ljungdahl pathway in Clostridium acetobutylicum enable the identification of the native tetrahydrofolate (THF) cycle and result in early induction of solvents", Metab. Eng., 77: 188-198 (2023.5), https://doi.org/10.1016/j.ymben.2023.04.005

675. Lim, J., Choi, S. Y., Lee, J. W., Lee, S. Y. & Lee, H., "Biohybrid CO2 electrolysis for the direct synthesis of polyesters from CO2", Proc. Nat. Acad. Sci. (PNAS), PNAS 2023 Vol. 120 No. 14 e2221438120, https://doi.org/10.1073/pnas.2221438120 (2023.4), https://doi.org/10.1073/pnas.2221438120

674. Kim, Y., Ryu, J.Y., Kim, H.U. and Lee, S.Y., "Computational prediction of interactions between Paxlovid and prescription drugs", Proc. Nat. Acad. Sci. (PNAS), PNAS 2023 Vol. 120 No. 12 e2221857120, https://doi.org/10.1073/pnas.2221857120 (2023.3), https://doi.org/10.1073/pnas.2221857120

673. Zhang, P, Gao, J., Zhang, H., Wang, Y., Liu, Z., Lee, S.Y., Mao, X., "Metabolic engineering of Escherichia coli for the production of an antifouling agent zosteric acid", Metab. Eng., 76: 247-259 (2023.3), https://doi.org/10.1016/j.ymben.2023.02.007

672. Kim, J.Y., Ahn, Y.-J., Lee, J.A., and Lee, S.Y. "Recent advances in the production of platform chemicals using metabolically engineered microorganisms", Curr. Opin. Green Sustain. Chem., 40:100777 1-14 (2023.4), https://doi.org/10.1016/j.tibtech.2022.12.014

671. Hu, B., Yu, H., Zhou, J., Li, J., Chen, J., Du, G., Lee, S.Y., and Zhao, X. "Whole-Cell P450 Biocatalysis using engineered Escherichia coli with fine-tuned heme biosynthesis", Adv. Sci., 10(6): 2205580 1-16, https://doi.org/10.1002/advs.202205580 (2023.2), https://doi.org/10.1002/advs.202205580

670. Kim, G.B., Choi, S.Y., Cho, I.J., Ahn, D.-H., and Lee, S.Y. "Metabolic engineering for sustainability and health", Trends Biotechnol. (the 40th year anniversary special issue), 41(3): 425-451(2023.3), https://doi.org/10.1016/j.tibtech.2022.12.014

669. Luo, Z.W., Choi, K.R., and Lee, S.Y. "Improved terephthalic acid production from p-xylene using metabolically engineered Pseudomonas putida", Metab. Eng., 76: 75-86 (2023.3), https://doi.org/10.1016/j.ymben.2023.01.007

668. Ahn, Y.-J., Lee, J.A., Choi, K.R., Bang, J., and Lee, S.Y. "Can microbes be harnessed to reduce atmospheric loads of greenhouse gases?", Environ. Microbiol., 25(1): 17-25 (2023.1), https://doi.org/10.1111/1462-2920.16161

667. Jang, W.D., Kim, G.B., and Lee, S.Y. "An interactive metabolic map of bio-based chemicals", Trends Biotechnol.,41(1): 10-14 (2023.1), https://doi.org/10.1016/j.tibtech.2022.07.013

666. Lee, J.A., Ahn, J.H., Kim, G.B., Choi, S., Kim, J.Y. and Lee, S.Y. "Metabolic engineering of Mannheimia succiniciproducens for malic acid production using dimethylsulfoxide as an electron acceptor", Biotechnol. Bioeng., 120(1): 203-215 (2023.1), https://doi.org/10.1002/bit.28242           

2022

665. Ghiffary, M.R., Prabowo, C.P.S., Adidjaja, J.J., Lee, S.Y., Kim, H.U., "Systems metabolic engineering of Corynebacterium glutamicum for the efficient production of β-alanine", Metab. Eng., 74: 121-129 (2022.11), https://doi.org/10.1016/j.ymben.2022.10.009

664. Choi, K.R., Yu, H.E., Lee, H., and Lee, S.Y. "Improved production of heme using metabolically engineered Escherichia coli", Biotechnol. Bioeng., 119(11): 3178-3193 (2022.11), https://doi.org/10.1002/bit.28194

663. Choi, K.R., Yu, H.E., and Lee, S.Y. "Production of zinc protoporphyrin IX by metabolically engineered Escherichia coli", Biotechnol. Bioeng., 119(11): 3319-3325 (2022.11), https://doi.org/10.1002/bit.28195

662. Cho, J.S., Kim, G.B., Eun, H.M., Moon, C.W., and Lee, S.Y. "Designing microbial cell factories for the production of chemicals", JACS Au, 2(8): 1781-1799 (2022.8), https://doi.org/10.1021/jacsau.2c00344

661. Park, S.Y., Eun, H., Lee, M.H., and Lee, S.Y. "Metabolic engineering of Escherichia coli with electron channelling for the production of natural products", Nature Catalysis, 5, 726-737 (2022.8), https://doi.org/10.1038/s41929-022-00820-4

660. Zou, X., Xia, X., Mo, Z., Ge, Y., Jiang, X., Huang, R., Li, M., Deng, Z., Chen, S., Wang, L., & Lee, S.Y. "Systematic strategies for developing phage resistant Escherichia coli strains", Nature Comm. 13: 4491 https://doi.org/10.1038/s41467-022-31934-9 (2022.2), https://doi.org/10.1038/s41467-022-31934-9

659. Yang, D., Eun, H., Prabowo, C.P.S., Cho, S., and Lee, S.Y. "Metabolic and cellular engineering for the production of natural products", Curr. Opin. Biotechnol, 77: 1-10, 102760 (2022.10), https://doi.org/10.1016/j.copbio.2022.102760

658. Koh, S., Choi, Y., Lee, I., Kim, G.-M., Kim, J., Park, Y.-S., Lee, S.Y., and Lee, D.C., "Light-Driven Ammonia Production by Azotobacter vinelandii Cultured in Medium Containing Colloidal Quantum Dots", J. Am. Chem. Soc., 144(24): 10798-10808 (2022.6), https://doi.org/10.1021/jacs.2c01886

657. Prabowo, C.P.S., Eun, H., Yang, D., Huccetogullari, D., Jegadeesh, R., Kim, S.J. and Lee, S.Y. "Production of natural colorants by metabolically engineered microorganisms", Trends Chem., 4(7): 608-626 (2022.7), https://doi.org/10.1016/j.trechm.2022.04.009

656. Chia, N., Lee, S.Y., and Tong, Y. "Optogenetic tools for microbial synthetic biology", Biotechnol. Adv., 59: 1-23 (2022. 10), https://doi.org/10.1016/j.biotechadv.2022.107953

655. Choi, K.R., Ahn, Y.-J., and Lee, S.Y. "Bacterial conversion of CO2 to organic compounds", J. CO2 Util., 58: 1-20 (2022.4), https://doi.org/10.1016/j.jcou.2022.101929

654. Fu, G., Yue, J., Li, D., Li, Y., Lee, S.Y., and Zhang, D. "An operator-based expression toolkit for Bacillus subtilis enables fine-tuning of gene expression and biosynthetic pathway regulation", Proc. Nat. Acad. Sci. (PNAS), PNAS 2022 Vol. 119 No. 11 e2119980119, https://doi.org/10.1073/pnas.2119980119 (2022.3), https://doi.org/10.1073/pnas.2119980119

653. Park, S.Y., Yang, D., Ha, S.H., and Lee, S.Y. "Production of phenylpropanoids and flavonolignans from glycerol by metabolically engineered Escherichia coli", Biotechnol. Bioeng., 119(3): 946-962 (2022.3), https://doi.org/10.1002/bit.28008

652. Jung, J.H., Cho, M., Seo, T.S., and Lee, S.Y. "Biosynthesis and applications of iron oxide nanocomposites synthesized by recombinant Escherichia coli", Appl. Microbiol. Biotechnol., 106: 1127-1137 (2022.1), https://doi.org/10.1007/s00253-022-11779-4

651. Jin, Q., Pan, F., Hu, C.-F., Lee, S.Y., Xia, X.-X., and Qian, Z.-G. "Secretory production of spider silk proteins in metabolically engineered Corynebacterium glutamicum for spinning into tough fibers", Metab. Eng., 70: 102-114 (2022.3), https://doi.org/10.1016/j.ymben.2022.01.009

650. Choi, K.R., Yu, H.E., Lee, S.Y., "Microbial food: microorganisms repurposed for our food", Microb. Biotechnol., 15(1): 18-25 (2022.1), https://doi.org/10.1111/1751-7915.13911           

2021

649. Yang, D., Zhou, H., and and Lee, S.Y., "Production of diversified polyketides by metabolic engineering", Biochemistry, 60(46): 3424-3426 (2021.11), https://doi.org/10.1021/acs.biochem.1c00290

648. Jang, Y.-S., Seong, H.J., Kwon, S.W., Lee, Y.-S., Im, J.A., Lee, H.L., Yoon, Y.R., and Lee, S.Y. "Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures", Front. Bioeng. Biotechnol. https://doi.org/10.3389/fbioe.2021.754250 (2021.10), https://doi.org/10.3389/fbioe.2021.754250

647. Gren, T., Whitford, C.M., Mohite, O.S., Jorgensen, T.S., Kontou, E.E., Nielsen, J.B., Lee, S.Y., and Weber, T. "Characterization and engineering of Streptomyces griseofuscus DSM 40191 as a potential host for heterologous expression of biosynthetic gene clusters", Sci. Rep. 11:18301 https://doi.org/10.1038/s41598-021-97571-2 (2021. 9), https://doi.org/10.1038/s41598-021-97571-2

646. Tong, Y., Jorgensen, T.S., Whitford, C.M., Weber, T., and Lee, S.Y. "A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing", Nature Comm. 12: 5206 https://doi.org/10.1038/s41467-021-25541-3 (2021. 9), https://doi.org/10.1038/s41467-021-25541-3

645. Kim, M.I., Park, C.Y., Seo, J.M., Kang, K.S., Park, K.S., Kang, J., Hong, K.S., Choi, Y., Lee, S.Y., Park, J.P., Park, H.G., and Park, T.J. "In situ biosynthesis of a metal nanoparticle encapsulated in alginate gel for imageable drug-delivery system", ACS Appl. Mater. Interfaces, 13(31): 36697-36708 (2021. 7), https://doi.org/10.1021/acsami.1c02286

644. Jang, W.D., Kim, G.B., Kim, Y., and Lee, S.Y. "Applications of artificial intelligence to enzyme and pathway design for metabolic engineering", Curr. Opin. Biotechnol., 73: 101-107 (2022. 2), https://doi.org/10.1016/j.copbio.2021.07.024

643. Zhu, Y., Luo, G., Wan, L., Meng, J., Lee, S.Y., and Mu, W. "Physiological effects, biosynthesis, and derivatization of key human milk tetrasaccharides, lacto-N-tetraose and lacto-N-neotetraose", Crit. Rev. Biotechnol., 42(4): 578-596 (2022. 5), https://doi.org/10.1080/07388551.2021.1944973

642. Choi, S.Y., Oh, J., Jung, J.H., Park, Y.K., and Lee, S.Y., "Three-dimensional label-free visualization and quantification of polyhydroxyalkanoates in individual bacterial cell in its native state", Proc. Natl. Acad. Sci. (PNAS), 118(31): e2103956118 (2021. 8), https://doi.org/10.1073/pnas.2103956118

641. Jang, W.D., Jeon, S., Kim, S. and Lee, S.Y., "Drugs repurposed for COVID-19 by virtual screening of 6,218 drugs and cell-based assay", Proc. Natl. Acad. Sci. (PNAS), 118(30): e2024302118 (2021. 7), https://doi.org/10.1073/pnas.2024302118

640. Yang, D., Park, S.Y., and Lee, S.Y., "Production of rainbow colorants by metabolically engineered Escherichia coli", Adv. Sci., 8(13): 2100743 1-13 (2021. 7), https://doi.org/10.1002/advs.202100743

639. Ghiffary, M.R., Prabowo, C.P.S., Komal, S., Yan, Y., Lee, S.Y. and Kim, H.U., "High-Level Production of the Natural Blue Pigment Indigoidine from Metabolically Engineered Corynebacterium glutamicum for Sustainable Fabric Dyes", ACS Sustainable Chem. Eng., 9(19): 6613-6622 (2021. 5), https://doi.org/10.1021/acssuschemeng.0c09341

638. Yang, D., Prabowo, C.P.S., Eun, H., Park, S.Y., Cho, I.J., Jiao, S., and Lee, S.Y."Escherichia coli as a platform microbial host for systems metabolic engineering", Essays Biochem., EBC20200172 65 (2): 225-246 (2021. 7), https://doi.org/10.1042/EBC20200172

637. Kim, Y., Ryu, J.Y., Kim, H.U., Jang, W.D., Lee, S.Y., "A deep learning approach to evaluate the feasibility of enzymatic reactions generated by retrobiosynthesis", Biotechnol. J., 16(5): 1-7 (2021. 5), https://doi.org/10.1002/biot.202000605

636. Bang, J., Ahn, J.H., Lee, J.A., Hwang, C.H., Kim, G.B., Lee, J., and Lee, S.Y. "Synthetic Formatotrophs for One-Carbon Biorefinery", Adv. Sci. 8, 2100199 (2021), https://doi.org/10.1002/advs.202100199

635. Jiang, X., Radko, Y., Gren, T., Palazzotto, E., Jogensen, T.S., Cheng, T., Xian, M., Weber, T., and Lee, S.Y. "Distribution of ε-poly-L-lysine synthetases in Coryneform bacteria Isolated from cheese and human skin", Appl. Environ. Microbiol., 87(10): e01841-20 1-8 (2021. 5), https://doi.org/10.1128/AEM.01841-20

634. Yang, D., Jang, W.D., and Lee, S.Y., "Production of carminic acid by metabolically engineered Escherichia coli", J. Am. Chem. Soc., 143(14): 5364-5377 (2021. 4), https://doi.org/10.1021/jacs.0c12406

633. Kim, Y., Kim, G.B., and Lee, S.Y., "Machine learning applications in genome-scale metabolic modeling", Curr. Opin. Sys. Biol., 25: 42-49 (2021. 3), https://doi.org/10.1016/j.coisb.2021.03.001

632. Fanguy, M., Lee, S.Y., and Churchill, D.G. "Adapting educational experiences for the chemists of tomorrow", Nature Rev. Chem. 5, 141-142. https://doi.org/10.1038/s41570-021-00258-5 (2021. 3), https://doi.org/10.1038/s41570-021-00258-5

631. Kim, G.B., Gao, Y., Palsson, B.E. and Lee, S.Y. "DeepTFactor: A deep learning-based tool for the prediction of transcription factors", Proc. Natl. Acad. Sci. (PNAS), 118(2): e2021171118 (2021. 1), https://doi.org/10.1073/pnas.2021171118

630. Davis, B., Backus, K., Winter, G., Chica, R., Li, D., Lee, S.Y., He, C., Weeks, A., Overall, C., Hagihara, S., Thuronyi, B., Kamat, S., Chen, L.-L., Guerrero, R.H., Yao, S., Mahal, L.K., Voigt, C., Woo, C., Strauss, E., Kikuchi, K., Dore, T., dford, S., Li, X.D., Heo, W.D., Superti-Furga, G., Deans, T., Belousov, V., Matthews, M., Jackson, C., Malek, S., Waldmann, H., Rising, A., Jewett, M., Stamou, D., Parker, E., Murakami, M., Polizzi, K., Hamachi, I., Erb, T., Joo, C., Uesugi, M., Prinjha, R., Rechavi, G., Solano, R., Schulman, B., Yael David, Y., Oslund, R., "Voices of chemical biology", feature, Nature Chem. Biol., 17: 1-4 (2021. 1), https://doi.org/10.1038/s41589-020-00714-1

629. Kim, D.I., Chae, T.U., Kim, H.U., Jang, W.D. & Lee, S.Y., "Microbial production of multiple short-chain primary amines via retrobiosynthesis", Nature Comm., 12: 173, DOI: 10.1038/s41467-020-20423-6 (2021. 1), https://doi.org/10.1038/s41467-020-20423-6                 

2016-2020

628. Komal, Ghiffary, M.R., Kim, H.U. and Lee, S.Y., "Engineering heterologous hosts for the enhanced production of non-ribosomal peptides", Biotechnol. Bioprocess Eng., 25: 795-809 (2020. 12) , https://doi.org/10.1007/s12257-020-0080-z

627. Jiang, X., Palazzotto, E., Wybraniec, E., Munro, L.J., Zhang, H., Kell, D.B., Weber, T. and Lee, S.Y., "Automating cloning by natural transformation", ACS Syn. Biol., 9(12): 3228-3235 (2020. 11), https://doi.org/10.1021/acssynbio.0c00240

626. Choi, Y., Lee, S.Y., "Biosynthesis of inorganic nanomaterials using microbial cells and bacteriophages", Nature Reviews Chemistry, 4(12): 638–656, https://doi.org/10.1038/s41570-020-00221-w (2020. 12) , https://doi.org/10.1038/s41570-020-00221-w

625. Park, S.Y., Yang, D., Ha, S.H., and Lee, S.Y. "Biosynthesis of dihydroquercetin in Escherichia coli from glycerol", BioRxiv, https://doi.org/10.1101/2020.11.27.401000 (2020.11.27), https://doi.org/10.1101/2020.11.27.401000

624. Han, T., Kim, G.B., and Lee, S.Y. "Glutaric acid production by systems metabolic engineering of an L-lysine–overproducing Corynebacterium glutamicum", Proc. Natl. Acad. Sci. (PNAS), 117(48): 30328-30334 (2020. 12), https://doi.org/10.1073/pnas.2017483117

623. Yoo, S.M., Jung, S.-W., Yeom, J., Lee, S.Y., and Na, D. "Tunable gene expression system independent of downstream coding sequence", ACS Syn. Biol., 9(11): 2998-3007 (2020. 10), https://doi.org/10.1021/acssynbio.0c00029

622. Hur, D.H., Choi, W.S., Kim, T.Y., Lee, S.Y., Park, J.H., Jeong, K.J., "Enhanced Production of Bacterial Cellulose in Komagataeibacter xylinus Via Tuning of Biosynthesis Genes with Synthetic RBS", J. Microbiol. Biotechnol., 30(9): 1430-1435 (2020. 9), https://doi.org/10.4014/jmb.2006.06026

621. Kim, S., Kim, Y., Suh, D.H., Lee, C.H., Yoo, S.M., Lee, S.Y., and Yoon, S.H. "Heat-responsive and time-resolved transcriptome and metabolome analyses of Escherichia coli uncover thermo-tolerant mechanisms", Sci. Rep., 10: 17715 (2020.10), https://doi.org/10.1038/s41598-020-74606-8

620. Luo, Z.W. and Lee, S.Y. "Metabolic engineering of Escherichia coli for the production of benzoic acid from glucose", Metab. Eng., 62: 298-311 (2020. 11), https://doi.org/10.1016/j.ymben.2020.10.002

619. Bang, J., Hwang, C.H., Ahn, J.H., Lee, J.A., and Lee, S.Y. "Escherichia coli is engineered to grow on CO2 and formic acid", Nature Microbiol., 5: 1459-1463, https://doi.org/10.1038/s41564-020-00793-9 (2020. 12), https://doi.org/10.1038/s41564-020-00793-9

618. Wei, S.-P., Qian, Z.-G., Hu, C.-F., Pan, F., Chen, M.-T., Lee, S.Y. and Xia, X.-X. "Formation and functionalization of membraneless compartments in Escherichia coli", Nature Chemical Biology, 16(10): 1143-1148 (2020. 10), https://doi.org/10.1038/s41589-020-0579-9

617. Choi, S.Y., Cho, I.J., Lee, Y., Kim, Y.-J., Kim, K.-J., and Lee, S.Y. "Microbial polyhydroxyalkanoates and non-natural polyesters", Adv. Mat., 32(35): 1907138, 1-37 (2020. 9), https://doi.org/10.1002/adma.201907138

616. Prabowo, C.P.S., Shin, J.H., Cho, J.S., Chae, T.U., and Lee, S.Y. "Microbial production of 4-amino-1-butanol, a four-carbon amino alcohol", Biotechnol. Bioeng., 117(9): 2771-2780 (2020. 9), https://doi.org/10.1002/bit.27438

615. Ko, Y.-S., Kim, J.W., Lee, J.A., Han, T., Kim, G.B., Park, J.E. and Lee, S.Y., "Tools and strategies of systems metabolic engineering for the development of microbial cell factories for chemical production", Chem. Soc. Rev., 49: 4615-4636 (2020. 7), https://doi.org/10.1039/d0cs00155d

614. Tong, Y., Whitford, C.M., Blin, K. Jorgensen, T.S., Weber, T., and Lee, S.Y. "CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes", Nature Protocols, 15: 2470-2502 (2020.7) (https://doi.org/10.1038/s41596-020-0339-z), https://doi.org/10.1038/s41596-020-0339-z

613. Kim, J.W., Ko, Y.-S., Chae, T.U., Lee, S.Y., "High-level production of 3-hydroxypropionic acid from glycerol as a sole carbon source using metabolically engineered Escherichia coli", Biotechnol. Bioeng., 117(7): 2139-2152 (2020. 6), https://doi.org/10.1002/bit.27344

612. Jang, W.D., Lee, S.M., Kim, H.U., and Lee, S.Y. "Systematic and comparative evaluation of software programs for template-based modeling of protein structures", Biotechnol. J., 15(6): 1900343 1-7 (2020. 6), https://doi.org/10.1002/biot.201900343

611. Choi, S.Y., Chae, T.U., Shin, J., Im, J.A., Lee, S.Y. "Biosynthesis and characterization of poly(d-lactate-co-glycolate-co-4-hydroxybutyrate)", Biotechnol. BioEng., 117(7): 2187-2197 (2020. 4), https://doi.org/10.1002/bit.27354

610. Yang, D., Park, S.Y., Park, Y.S., Eun, H., and Lee, S.Y. "Metabolic engineering of E. coli for natural products biosynthesis", Trends Biotechnol., 38(7): 745-765 (2020. 7), https://doi.org/10.1016/j.tibtech.2019.11.007

609. Ko, Y.-S., Kim, J.W., Chae, T.U., Song, C.W., and Lee, S.Y. "A novel biosynthetic pathway for the production of acrylic acid through β-alanine route in Escherichia coli", ACS Syn. Biol., 9(5): 1150-1159 (2020. 4), https://doi.org/10.1021/acssynbio.0c00019

608. Ahn, J.H., Seo, H., Park, W., Seok, J., Lee, J.A., Kim, W.J., Kim, G.B., Kim, K.-J. & Lee, S.Y., "Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase", Nature Comm., 11: 1970, DOI: 10.1038/s41467-020-15839-z (2020. 4), https://doi.org/10.1038/s41467-020-15839-z

607. Choi, K.R., Jiao, S., and Lee, S.Y. "Metabolic engineering strategies toward production of biofuels", Curr. Opin. Chem. Biol., 59: 1-14 (2020. 12), https://doi.org/10.1016/j.cbpa.2020.02.009

606. Lee, J.A., Ahn, J.H., Kim, I., Li, S., and Lee, S.Y. "Synthesis, characterization, and application of fully bio-based and biodegradable nylon-4,4 and -5,4", ACS Sustain. Chem. Eng., 8(14): 5604-5614 (2020. 4), https://doi.org/10.1021/acssuschemeng.0c00007

605. Cho, I.J., Choi, K.R., and Lee, S.Y. "Microbial production of fatty acids and derivative chemicals", Curr. Opin. Biotechnol., 65: 129-141 (2020. 10), https://doi.org/10.1016/j.copbio.2020.02.006

604. Lee, S.H. and Lee, S.Y. "Cell Surface Display of Poly(3-hydroxybutyrate) Depolymerase and its Application", J. Microbiol. Biotechnol., 30(2): 244-247 (2020. 2), https://doi.org/10.4014/jmb.2001.01042

603. Lieven, C., Beber, M.E., Olivier, B.G., Bergmann, F.T., Ataman, M., Babaei, P., Bartell, J.A., Blank, L.M., Chauhan, S., Correia, K., Diener, C., Dräger, A., Ebert, B.E., Edirisinghe, J.N., Faria, J.P., Feist, A.M., Fengos, G., Fleming, R.M.T., García-Jiménez, B., Hatzimanikatis, V., Helvoirt, W.V., Henry, C.S., Hermjakob, H., Herrgård. M.J., Kaafarani, A., Kim, H.U., King, Z., Klamt, S., Klipp, E., Koehorst, J.J., König, M., Lakshmanan, M., Lee, D.-Y., Lee, S.Y., Lee, S., Lewis, N.E., Liu, F., Ma, H., Machado, D., Mahadevan, R., Maia, P., Mardinoglu, A., Medlock, G.L., Monk, J.M., Nielsen, J., Nielsen, L.K., Nogales, J., Nookaew, I., Palsson, B.O., Papin, J.A., Patil, K.R., Poolman, M., Price, N.D., Resendis-Antonio, O., Richelle, A., Rocha, I., Sánchez, B.J., Schaap, P.J., Sheriff, R.S.M., Shoaie, S., Sonnenschein, N., Teusink, B., Vilaça, P., Vik, J.O., Wodke, J.A.H., Xavier, J.C., Yuan, Q., Zakhartsev, M., and Zhang, C. "MEMOTE for standardized genome-scale metabolic model testing", Nature Biotechnol., 38(3): 272-276 (2020.03), https://doi.org/10.1038/s41587-020-0446-y

602. Choi, S.Y., Rhie, M.N., Kim, H.T., Joo, J.C., Cho, I.J., Son, J., Jo, S.Y., Shon, Y.J., Baritugo, K.-A., Pyo, J., Lee, Y., Lee, S.Y., and Park, S.J. "Metabolic engineering for the synthesis of polyesters: A 100-year journey from polyhydroxyalkanoates to non-natural microbial polyesters", Metab. Eng., 58: 47-81 (2020. 3), https://doi.org/10.1016/j.ymben.2019.05.009

601. Chae, T.U., Ahn, J.H., Ko, Y.-S., Kim, J.W., Lee, J.A., Lee, E.H., and Lee, S.Y. "Metabolic engineering for the production of dicarboxylic acids and diamines", Metab. Eng., 58: 2-16 (2020. 3), https://doi.org/10.1016/j.ymben.2019.03.005

600. Hur, D.H., Rhee, H.-S., Lee, J.H., Shim, W.Y., Kim, T.Y., Lee, S.Y., Park, J.H., and Jeong, K.J. "Enhanced production of cellulose in Komagataeibacter xylinus by preventing insertion of IS element into cellulose synthesis gene", Biochem. Eng. J., 156: 1-5 (2020. 4), https://doi.org/10.1016/j.bej.2020.107527

599. Jakočiūnasa, T., Klitgaard, A.K., Kontou, E.E., Nielsen, J.B., Thomsen, E., Romero-Suarez, D., Blin, K., Petzold, C.J., Gin, J.W., Tong, Y., Gotfredsen, C.H., Charusanti, P., Frandsen, R.J.N., Weber, T., Lee, S.Y., Jensen, M.K., Keasling, J.D., "Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast", Synth. Syst. Biotechnol., 5(1): 11-18 (2020. 3), https://doi.org/10.1016/j.synbio.2020.01.004

598. Kim, H.T., Baritugo, K.-A., Hyun, S.M., Khang, T.U., Sohn, Y.J., Kang, K.H., Jo, S.Y., Song, B.K., Park, K., Kim, I.-K., Hwang, Y.T., Lee, S.Y., Park, S.J., and Joo, J.C. "Development of metabolically engineered Corynebacterium glutamicum for enhanced production of cadaverine and its use for the synthesis of bio-polyamide 510", ACS Sustain. Chem. Eng., 8(1): 129−138 (2020. 1), https://doi.org/10.1021/acssuschemeng.9b04693

597. Kim, Y., Gu, C., Kim, H.U., and Lee, S.Y. "Current status of pan-genome analysis for pathogenic bacteria", Curr. Opin. Biotechnol., 63: 54-62 (2020. 6), https://doi.org/10.1016/j.copbio.2019.12.001

596. Kim, G.B., Kim, W.J., Kim, H.U., and Lee, S.Y. "Machine learning applications in systems metabolic engineering", Curr. Opin. Biotechnol., 64: 1-9 (2020. 8), https://doi.org/10.1016/j.copbio.2019.08.010

595. Choi, K.R. and Lee, S.Y. "Protocols for RecET-based markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida", Microb. Biotechnol., 13(1): 199-209 (2020.1), https://doi.org/10.1111/1751-7915.13374

594. Kwon, M.S., Lee, B.T., Lee, S.Y., and Kim, H.U. "Modeling regulatory networks using machine learning for systems metabolic engineering", Curr. Opin. Biotechnol., 65: 163-170 (2020. 10), https://doi.org/10.1016/j.copbio.2020.02.014

593. Woo, J.E., Seong, H.J., Lee, S.Y., and Jang, Y.-S. "Metabolic engineering of Escherichia coli for the production of hyaluronic acid from glucose and galactose", Front. Bioeng. Biotech., 7(351): 1-9 (2019. 11), https://doi.org/10.3389/fbioe.2019.00351

592. Jang, W.D., Kim, T.Y., Kim, H.U., Shim, W.Y., Ryu, J.Y., Park, J.H., and Lee, S.Y. "Genomic and metabolic analysis of Komagataeibacter xylinus DSM 2325 producing bacterial cellulose nanofiber", Biotechnol. Bioeng., 116(12): 3372-3381 (2019. 12), https://doi.org/10.1002/bit.27150

591. Seo, H., Cho, I.J., Joo, S., Son, H.F., Sagong, H.-Y., Choi, S.Y., Lee, S.Y., & Kim, K.-J., "Reply to "Conformational fitting of a flexible oligomeric substrate does not explain the enzymatic PET degradation" Nature Comm., 10:5582, 1-2, DOI: 10.1038/s41467-019-13492-9 (2019.12), https://doi.org/10.1038/s41467-019-13492-9

590. Tong, Y., Whitford, C.M., Robertsen, H.L., Blin, K., Jørgensen, T.S., Klitgaard, A.K., Gren, T., Jiang, X., Weber, T., and Lee, S.Y., "Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST", Proc. Natl. Acad. Sci. (PNAS), 116 (41): 20366–20375, (https://doi.org/10.1073/pnas.1913493116) (2019. 10), https://doi.org/10.1073/pnas.1913493116

589. Gwon, H., Park, K., Chung, S.-C., Kim, R.-H., Kang, J.K., Ji, S.M., Nag-Jong Kim, Lee, S., Ku, J.-H., Do, E.C., Park, S., Kim, M., Shim, W.Y., Rhee, H.S., Kim, J.-Y., Kim, J., Kim, T.Y., Yamaguchi, Y., Iwamuro, R., Saito, S., Kim, G., Jung, I.-S., Park, H., Lee, C., Lee, S., Jeon, W.S., Jang, W.D., Kim, H.U., Lee, S.Y., Im, D., Doo, S.-G., Lee, S.Y., Lee, H.C. and Park, J.H., "A safe and sustainable bacterial cellulose nanofiber separator for lithium rechargeable batteries", Proc. Natl. Acad. Sci. (PNAS), 116 (37): 19288-19293, (https://doi.org/10.1073/pnas.1905527116) (2019. 9), https://doi.org/10.1073/pnas.1905527116

588. Song, C.W., Park, J.M., Chung, S.C., Lee, S.Y., Song, H., "Microbial production of 2,3‑butanediol for industrial applications", J. Ind. Microbiol. Biotechnol., 46(11): 1-19 (2019. 11), https://doi.org/10.1007/s10295-019-02231-0

587. Palazzotto, E., Tong, Y., Lee, S.Y., and Weber, T. "Synthetic biology and metabolic engineering of actinomycetes for natural product discovery", Biotechnol. Adv., 37(6), 1-15 (2019.11), https://doi.org/10.1016/j.biotechadv.2019.03.005

586. Choi, K.R., Jang, W.D., Yang, D., Cho, J.S., Park, D., and Lee, S.Y. "Systems Metabolic Engineering Strategies: Integrating Systems and Synthetic Biology with Metabolic Engineering", Trends Biotechnol., 37(8): 817-837 (2019.8), https://doi.org/10.1016/j.tibtech.2019.01.003

585. Wang, F., Wang, L., Zou, X., Duan, S., Li, Z., Deng, Z., Luo, J., Lee, S.Y., and Chen, S. "Advances in CRISPR-Cas systems for RNA targeting, tracking and editing", Biotechnol. Adv., 37(5): 708-729 (2019.9), https://doi.org/10.1016/j.biotechadv.2019.03.016

584. Blin, K., Shaw, S., Steinke, K., Villebro, R., Ziemert, N., Lee, S.Y., Medema, M.H., Weber, T. "antiSMASH 5.0: updates to the secondary metabolite genome mining pipeline", Nucl. Acids Res., 47(W1): W81-W87 (2019.7), https://doi.org/10.1093/nar/gkz310

583. Kim, H.M., Chae, T.U., Choi, S.Y., Kim, W.J. and Lee, S.Y., "Engineering of an oleaginous bacterium for the production of fatty acids and fuels", Nature Chem. Biol., 15, 721-729 (https://doi.org/10.1038/s41589-019-0295-5) (2019.6), https://doi.org/10.1038/s41589-019-0295-5

582. Ryu, J.Y., Kim, H.U. and Lee, S.Y., "Deep learning enables high-quality and high-throughput prediction of enzyme commission numbers", Proc. Natl. Acad. Sci. (PNAS), 116 (28): 13996-14001 (https://doi.org/10.1073/pnas.1821905116) (2019.7), https://doi.org/10.1073/pnas.1821905116

581. Luo, Z.W., Cho, J.S., Lee, S.Y. "Microbial production of methyl anthranilate, a grape flavor compound", Proc. Natl. Acad. Sci. (PNAS), 116 (22): 10749-10756 (https://doi.org/10.1073/pnas.1903875116 (2019.5), https://doi.org/10.1073/pnas.1903875116

580. Heo, N.S., Shukl, S., Oh, S.Y., Bajpai, V.K., Lee, S.U., Cho, H.-J., Kim, S., Kim, Y., Kim, H.J., Lee, S.Y., Jun, Y.-S., Oh, M.-W., Han, Y.-K., Yoo, S.M., and Huh, Y.S. “Shape-controlled assemblies of graphitic carbon nitride polymer for efficient sterilization therapies of water microbial contamination via 2D g-C3N4 under visible light illumination”, Mater. Sci. Eng. C, 104: 109846, 1-9, https://doi.org/10.1016/j.msec.2019.109846 (2019.11), https://doi.org/10.1016/j.msec.2019.109846

579. Gu, C., Kim, G.B., Kim, W.J., Kim, H.U., and Lee, S.Y."Current status and applications of genome-scale metabolic models", Genome Biol. 20(121): 1-18, https://doi.org/10.1186/s13059-019-1730-3 (2019. 12), https://doi.org/10.1186/s13059-019-1730-3

578. Timmis, K., Cavicchioli, R., Garcia, J.L., Nogales, B., Chavarrias, M., Stein, L., McGenity, T.J., Websters, N., Singh, B., Handelsman, J., de Lorenzo, V., Pruzzo, C., Timmis, J., Martin, J.L.R., Verstraete, W., Jetten, M., Danchin, A., Huang, W., Gilberts, J., Lal, R., Santos, H., Lee, S.Y., Sessitsch, A., Bonfante, P., Gram, L., Lin, R.T.P., Ron, E., Karahan, C., van der Meer, J.R., Artunkal, S., Jahn, D., and Harper, L. "The urgent need for microbiology literacy in society", Environ. Microbiol., 21(5): 1513-1528 (2019.5), https://doi.org/10.1111/1462-2920.14611

577. Yang, D., Yoo, S.M., Gu, C., Ryu, J.Y., Lee, J.E., and Lee, S.Y., "Expanded synthetic small regulatory RNA expression platforms for rapid and multiplex gene expression knockdown", Metab. Eng., 54: 180-190 (2019.07), https://doi.org/10.1016/j.ymben.2019.04.003

576. Cho, J.S., Gu, C., Han, T.H., Ryu, J.Y., and Lee, S.Y., "Reconstruction of context-specific genome-scale metabolic models using multiomics data to study metabolic rewiring", Curr. Opin. Sys. Biol., 15: 1-11 (2019. 6), https://doi.org/10.1016/j.coisb.2019.02.009

575. Noh, M., Yoo, S.M., Yang, D., and Lee, S.Y. "Broad-spectrum gene repression using scaffold engineering of synthetic sRNAs", ACS Syn. Biol., 8(6): 1452-1461 (2019.6), https://doi.org/10.1021/acssynbio.9b00165

574. Son, H.F., Cho, I.J., Joo, S., Seo, H., Sagong, H.-Y., Choi, S.Y., Lee, S.Y., and Kim, K.-J. "Rational protein engineering of thermo-stable PETase from Ideonella sakaiensis for highly efficient PET degradation", ACS Catal., 9(4): 3519-3526 (2019. 3), https://doi.org/10.1021/acscatal.9b00568

573. Lee, Y., Cho, I.J., Choi, S.Y., and Lee, S.Y. "Systems metabolic engineering strategies for non-natural microbial polyester production", Biotechnol. J., 1800426: 1-12 (2019. 3), https://doi.org/10.1002/biot.201800426

572. Zhao, X., Park, S.Y., Yang, D., and Lee, S.Y. "Synthetic biology for natural compounds", Biochemistry, 58: 1454-1456 (2019.03), https://doi.org/10.1021/acs.biochem.8b00569

571. Noh, H.J., Lee, S.Y., Jang, Y.S., "Microbial production of butyl butyrate, a flavor and fragrance compound" Appl. Microbiol. Biotechnol., 103(5): 2079-2086 (2019. 3), https://doi.org/10.1007/s00253-018-09603-z

570. Huccetogullari, D., Luo, Z.W., and Lee, S.Y. "Metabolic engineering of microorganisms for production of aromatic compounds", Microb. Cell Fact., 18(41): 1-29 (2019. 2), https://doi.org/10.1186/s12934-019-1090-4

569. Lee, J.A., Ahn, J.H., Kim, I., Li, S., and Lee, S.Y. "Separation and purification of three, four, and five carbon diamines from fermentation broth", Chem. Eng. Sci., 196: 324-332 (2019. 3), https://doi.org/10.1016/j.ces.2018.11.018

568. Blin, K., Andreu, V.P., de los Santos, E.L.C., Carratore, F.D., Lee, S.Y., Medema, M.H., and Weber, T. "The antiSMASH database version 2 - A comprehensive resource on secondary metabolite biosynthetic gene clusters", Nucl. Acids Res., 47(D1): D625-630 (2019. 1), https://doi.org/10.1093/nar/gky1060

567. Kim, H.T., Khang, T.U., Baritugo, K.-A., Hyun, S.M., Kang, K.H., Jung, S.H., Song, B.K., Park, K., Oh, M.-K., Kim, G.B., Kim, H.U., Lee, S.Y., Park, S.J., Joo, J.C. "Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical", Metab. Eng., 51: 99-109 (2019. 1) , https://doi.org/10.1016/j.ymben.2018.08.007

566. Cho, C., Hong, S., Moon, H.G., Jang, Y.-S., Kim, D., and Lee, S.Y. "Engineering Clostridial Aldehyde/Alcohol Dehydrogenase for Selective Butanol Production", mBio, 10(1) e02683-18 1-12 (2019. 1), https://doi.org/10.1128/mBio.02683-18

565. Lee, S.Y., Kim, H.U., Chae, T.U., Cho, J.S., Kim, J.W., Shin, J.H., Kim, D.I., Ko, Y.-S., Jang, W.D. and Jang, Y.-S., "A comprehensive metabolic map for production of bio-based chemicals" Nature Catalysis, 2: 18-33 (2019.1), https://doi.org/10.1038/s41929-018-0212-4

564. Mohite, O.S., Weber, T., Kim, H.U., and Lee, S.Y. "Genome-scale metabolic reconstruction of actinomycetes for antibiotics production", Biotechnol. J., 14(1): 1800377 1-9, (2019. 1), https://doi.org/10.1002/biot.201800377

563. Tong, Y., Weber, T., and Lee, S.Y. "CRISPR/Cas-based genome engineering in natural product discovery", Nat. Prod. Rep., 36(9):1262-1280 (2019.9), https://doi.org/10.1039/c8np00089a

562. Choi, S.Y., Cho, I.J., Lee, Y.J., Park, S., and Lee, S.Y. "Biocatalytic synthesis of polylactate and its copolymers", Methods Enzymol., 627: 125-162 (2019.5), https://doi.org/10.1016/bs.mie.2019.04.032

561. Yang, J.E., Park, S.J., Kim, W.J., Kim, H.J., Kim, B.J., Lee, H., Shin, J., & Lee, S.Y., "One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains", Nature Comm., 9:79, DOI: 10.1038/s41467-017-02498-w (2018.1), https://doi.org/10.1038/s41467-017-02498-w

560. Robertsen, H.L., Weber, T., Kim, H.U., and Lee, S.Y., "Towards systems metabolic engineering of Streptomycetes for secondary metabolites production", Biotechnol. J., 13(1): 1-12 (2018. 1), https://doi.org/10.1002/biot.201700465

559. Du, J., Yang, D., Luo, Z.W., and Lee, S.Y. "Metabolic Engineering of Escherichia coli for the Production of Indirubin from Glucose", J. Biotechnol., 267: 19-28 (2018. 2), https://doi.org/10.1016/j.jbiotec.2017.12.026

558. Chae, T.U., Choi, S.Y., Ryu, J.Y., and Lee, S.Y. "Production of ethylene glycol from xylose by metabolically engineered Escherichia coli", AIChE J., 64(12): 4193-4200 (2018. 12), https://doi.org/10.1002/aic.16339

557. Jung, J.H., Lee, S.Y., and Seo, T.S. “In vivo synthesis of nanocomposites using the recombinant Escherichia coli”, Small, 14(42): 1803133, 1-7 (2018. 10), https://doi.org/10.1002/smll.201803133

556. O’Day, E., Hosta-Rigau, L., Oyarzún, D.A., Okano, H., de Lorenzo, V., von Kameke C., Alsafar, H., Cao, C., Chen, G.-Q., Ji, W., Roberts, R., Ronaghi, M., Yeung, K., Zhang, F., and Lee, S.Y. "Are we there yet? How and when specific biotechnologies will improve human health", Biotechnol. J., 1800195: 1-11 (2018. 11), https://doi.org/10.1002/biot.201800195

555. Kim, B., Binkley, R., Kim, H.U., and Lee, S.Y. "Metabolic engineering of Escherichia coli for the enhanced production of L-tyrosine", Biotechnol. Bioeng, 115(10): 2554-2564 (2018. 10), https://doi.org/10.1002/bit.26797

554. Sagong, H.-Y., Son, H.F., Choi, S.Y., Lee, S.Y., and Kim, K.-J., "Structural insights into polyhydroxyalkanoates biosynthesis", Trends Biochem. Sci., 43(10): 790-805 (2018. 10), https://doi.org/10.1016/j.tibs.2018.08.005

553. Luo, Z.W., Kim, W.J., and Lee, S.Y. "Metabolic engineering of Escherichia coli for efficient production of 2-pyrone-4,6-dicarboxylic acid from glucose", ACS Syn. Biol., 7(9): 2296-2307 (2018. 9), https://doi.org/10.1021/acssynbio.8b00281

552. Choi, K.R., Ryu, J.Y., and Lee, S.Y. "Revisiting statistical design and analysis in scientific research", Small, 14(40): 1802604 (1-8) (2018. 10), https://doi.org/10.1002/smll.201802604

551. Yang, D., Kim, W.J., Yoo, S.M., Choi, J.H., Ha, S.H., Lee, M.H., and Lee, S.Y. "Repurposing type III polyketide synthase as a malonyl-CoA biosensor for metabolic engineering in bacteria", Proc. Natl. Acad. Sci. (PNAS), 115 (40) 9835-9844 (https://doi.org/10.1073/pnas.1808567115) (2018.10.2), https://doi.org/10.1073/pnas.1808567115                               

550. Noh, H.J., Woo, J.E., Lee, S.Y., and Jang, Y.-S. "Metabolic engineering of Clostridium acetobutylicum for the production of butyl butyrate", Appl. Microbiol. Biotechnol., 102(19): 8319-8327 (2018. 10), https://doi.org/10.1007/s00253-018-9267-z

549. Bang, J., Lee, S.Y. "Assimilation of formic acid and CO2 by engineered Escherichia coli equipped with reconstructed one-carbon assimilation pathways", Proc. Nat. Acad. Sci. (PNAS), 115 (40) E9271-E9279 (https://doi.org/10.1073/pnas.1810386115) (2018.10.2), https://doi.org/10.1073/pnas.1810386115

548. Zhao, X.R., Choi, K.R., and Lee, S.Y. "Metabolic engineering of Escherichia coli for secretory production of free haem", Nature Catalysis, 1:720-728 (2018), https://doi.org/10.1038/s41929-018-0126-1

547. Choi, K.R., Kim, W.J., and Lee, S.Y. "Metabolomics for industrial fermentation", Bioproc. Biosys. Eng., 41(7): 1073-1077 (2018. 7), https://doi.org/10.1007/s00449-018-1967-3

546. Park, S.Y., Binkley, R.M., Kim, W.J., Lee, M.H., and Lee, S.Y. "Metabolic engineering of Escherichia coli for high-level astaxanthin production with high productivity", Metab. Eng., 49: 105-115 (2018. 9), https://doi.org/10.1016/j.ymben.2018.08.002

545. Woo, J.E., Lee, S.Y., and Jang, Y.-S. "Effects of nutritional enrichment on acid production from degenerated (non-solventogenic) Clostridium acetobutylicum strain M5", Appl. Biol. Chem.. 61(4): 469-472 (2018. 8), https://doi.org/10.1007/s13765-018-0372-6

544. Choi, Y., Park, T.J., Lee, D.C., and Lee, S.Y. "Recombinant Escherichia coli as a biofactory for various single- and multi-element nanomaterials", Proc. Nat. Acad. Sci. (PNAS), 115(23): 5944-5949 (2018. 6), https://doi.org/10.1073/pnas.1804543115

543. Choi, K.R., Cho, J.S., Cho, I.J., Park, D., and Lee, S.Y. "Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida", Metab. Eng., 47: 463-474 (2018. 5), https://doi.org/10.1016/j.ymben.2018.05.003

542. Ryu, J.Y., Kim, H.U., and Lee, S.Y. "Deep learning improves prediction of drug–drug and drug–food interactions", Proc. Nat. Acad. Sci. (PNAS), 201803294: 1-8 (2018. 5), https://doi.org/10.1073/pnas.1803294115

541. Hwang, H.J., Lee, S.Y., and Lee, P.C. "Engineering and application of synthetic nar promoter for fine tuning the expression of metabolic pathway genes in Escherichia coli", Biotechnol. Biofuels (2018) 11:103, DOI:10.1186/s13068-018-1104-1 (2018. 4), https://doi.org/10.1186/s13068-018-1104-1

540. De Lorenzo, V., Prather, K., Chen, G.-Q., O’Day, E., von Kameke, C., Oyarzun, D.A., Hosta-Rigau, L., Alsafar, H., Cao, C., Ji, W., Okano, H., Roberts, R.J., Ronaghi, M., Yeung, K., Zhang, F., and Lee, S.Y. "The power of synthetic biology for bioproduction, remediation and pollution control", EMBO Rep., 19, e45658 (2018. 4), https://doi.org/10.15252/embr.201745658

539. Tong, T., Chen, S., Wang, L., Tang, Y., Ryu, J.Y., Jiang, S., Wu, X., Chen, C., Luo, J., Deng, Z., Li, Z., Lee, S.Y., and Chen, S. "Occurrence, evolution, and functions of DNA phosphorothioate epigenetics in bacteria", Proc. Nat. Acad. Sci. (PNAS),  115(13): E2988-E2996 (2018. 3), https://doi.org/10.1073/pnas.1721916115

538. Chen, X.-F., Xia, X.-X., Lee, S.Y., and Qian, Z.-G., "Engineering tunable biosensors for monitoring putrescine in Escherichia coli", Biotechnol. Bioeng., 115(4): 1014-1027 (2018. 4), https://doi.org/10.1002/bit.26521

537. Choi, Y., Hwang, J.H., and Lee, S.Y. "Recent trends in nanomaterials-based colorimetric detection of pathogenic bacteria and viruses", Small Methods, 1700351: 1-9 (2018. 4), https://doi.org/10.1002/smtd.201700351

536. Robertsen, H.L., Musiol-Kroll, E.M., Ding, L., Laiple, K.J., Hofeditz, T., Wohlleben, W., Lee, S.Y., Grond, S., and Weber, T. "Filling the gaps in the kirromycin biosynthesis: deciphering the role of genes involved in ethylmalonyl-CoA supply and tailoring reactions", Sci. Rep., 8:3230, DOI:10.1038/s41598-018-21507-6 (2018. 2), https://doi.org/10.1038/s41598-018-21507-6

535. Ahn, J.H., Lee, J.A., Bang, J., and Lee, S.Y., "Membrane engineering via trans-unsaturated fatty acids production improves succinic acid production in Mannheimia succiniciproducens", J. Ind. Microbiol. Biotechnol., 45(7): 555-566 (2018. 7), https://doi.org/10.1007/s10295-018-2016-6

534. Park, S.Y., Yang, D., Ha, S.H. and Lee, S.Y., "Metabolic Engineering of Microorganisms for the Production of Natural Compounds", Adv. Biosys., 2(1): 1700190 (2018. 1), https://doi.org/10.1002/adbi.201700190

533. Joo, S., Cho, I.J., Seo, H., Son, H.F., Sagong, H.-Y., Shin, T.J., Choi, S.Y., Lee, S.Y. & Kim, K.J., "Structural insight into molecular mechanism of poly (ethylene terephthalate) degradation", Nature Comm., 9:382, DOI: 10.1038/s41467-018-02881-1 (2018.1), https://doi.org/10.1038/s41467-018-02881-1

532. Park, S., Jun, S.Y., Kim, C.-H., Jung, G.M., Son, J.S., Jeong, S.T., Yoon, S.J., Lee, S.Y. & Kang, S.H., "Characterisation of the antibacterial properties of the recombinant phage endolysins AP50-31 and LysB4 as potent bactericidal agents against Bacillus anthracis" Sci. Rep., 8: 18, DOI:10.1038/s41598-017-18535-z (2018. 1), https://doi.org/10.1038/s41598-017-18535-z

531. Timmis, K., de Vos, W.M., Ramos, J.L., Vlaeminck, S.E., Prieto, A., Danchin, A., Verstraete, W., de Lorenzo, V., Lee, S.Y., Br€ussow, H., Timmis, J.K., and Singh, B.K., "The contribution of microbial biotechnology to sustainable development goals", Microb. Biotechnol. 10(5): 984-987 (2017.8), https://doi.org/10.1111/1751-7915.12818

530. Kim, Y-J., Choi, S.Y., Kim, J.E., Jin, K.S., Lee, S.Y., Kim, K-J., "Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme", Biotechnol. J. 12, 1600648, DOI 10.1002/biot.201600649 (2017.1), http://dx.doi.org/10.1002/biot.201600649

529. Kim, J.E., Kim, Y-J., Choi, S.Y., Lee, S.Y., Kim, K-J., "Crystal structure of Ralstonia eutropha polyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms", Biotechnol. J. 12, 1600648, DOI 10.1002/biot.201600648 (2017.1), http://dx.doi.org/10.1002/biot.201600648

528. David, Y., Joo, J.C., Yang, J.E., Oh, Y.H., Lee, S.Y., and Park, S.J. "Biosynthesis of 2-hydroxyacid-containing polyhydroxyalkanoates by employing butyryl-CoA transferases in metabolically engineered Escherichia coli", Biotechnol. J., 12(11) 1-11(2017.11), http://dx.doi.org/10.1002/biot.201700116

527. Choi, S.Y., Kim, W.J., Yu, S.J., Park, S.J., Im, S.G., and Lee, S.Y. "Engineering the xylose-catabolizing Dahms pathway for production of poly(D-lactate-co-glycolate) and poly(D-lactate-co-glycolate-co- D-2-hydroxybutyrate) in Escherichia coli", Microbial Biotechnol., 10(6): 1353-1364 (2017.11), http://dx.doi.org/10.1111/1751-7915.12721

526. Mao, X., Liu, Z., Sun, J., and Lee, S.Y., "Metabolic engineering for the microbial production of marine bioactive compounds", Biotechnol. Adv., 35(8): 1004-1021 (2017.12), http://dx.doi.org/10.1016/j.biotechadv.2017.03.001

525. Kim, H.U., Blin, K., Lee, S.Y., and Weber, T. "Recent development of computational resources for new antibiotics discovery", Curr. Opin. Microbiol., 39: 113-120 (October 2017), http://dx.doi.org/10.1016/j.mib.2017.10.027

524. Ahn, J.H., Bang, J., Kim, W.J., and Lee, S.Y. "Formic acid as a secondary substrate for succinic acid production by metabolically engineered Mannheimia succiniciproducens", Biotechnol. Bioeng. 114(12):2837-2847 (2017. 12), https://doi.org/10.1002/bit.26435

523. Ryu, J.Y., Kim, H.U., and Lee, S.Y., "Framework and resource for more than 11,000 gene-transcript-protein-reaction associations in human metabolism", Proc. Nat. Acad. Sci. (PNAS)., 114: E9740-E9749 (October 24, 2017), https://doi.org/10.1016/j.ymben.2017.09.001

522. Liu, J., Wang, Z., Kandasamy, V., Lee, S.Y., Solem, C., and Jensen, P.R. "Harnessing the respiration machinery for high-yield production of chemicals in metabolically engineered Lactococcus lactis", Metab. Eng. 44: 22-29 (2017. 11), https://doi.org/10.1016/j.ymben.2017.09.001

521. Ahn, J.-H., Choi, S.-J., Im, M., Kim, S., Kim, C.-H., Kim, J.-Y., Park, T.J., Lee, S.Y., and Choi, Y.-K. "Charge and dielectric effects of biomolecules on electrical characteristics of nanowire FET biosensors", Appl. Phys. Lett. 111, 113701 (2017); doi: 10.1063/1.5003106 (September 2017), http://dx.doi.org/10.1063/1.5003106

520. Joo, J.C., Oh, Y.H., Yu, J.H., Hyun, S.M., Khang, T.U., Kang, K.H., Song, B.K., Park, K., Oh, M.-K., Lee, S.Y., and Park, S.J. "Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process", Bioresource Technol., 245: 1692-1700 (2017. 12), https://doi.org/10.1016/j.biortech.2017.05.131

519. Kim, D.-K., Kim, D. M., Yoo. S. M., and Lee, S. Y. "Controllable gold-capped nanoporous anodic alumina chip for label-free, specific detection of bacterial cells", RSC Adv., 2017, 7, 18815-18820 (March 2017), https://doi.org/10.1039/c6ra27130h

518. Kim, S., Jeong, H., Kim, E.-Y., Kim, J.F., Lee, S.Y., and Yoon, S.H. "Genomic and transcriptomic landscape of Escherichia coli BL21(DE3)", Nucleic Acids Res., 45(9) 5285-5293 (2017. 5), https://doi.org/10.1093/nar/gkx228

517. Noh, M., Yoo, S.M., Kim, W.J., and Lee, S.Y. "Gene expression knockdown by modulating synthetic small RNA expression in Escherichia coli", Cell Systems, https://doi.org/10.1016/j.cels.2017.08.016

516. Jang, W.D., Hwang, J.H., Kim, H.U., Ryu, J.Y., and Lee, S.Y. "Bacterial cellulose as an example product for sustainable production and consumption", Microb. Biotechnol., 10(5), 1181-1185 (2017.09), http://dx.doi.org/10.1111/1751-7915.12744

515. Yang, D.S., Cho, J.S., Choi, K.R., Kim, H.U., and Lee, S.Y., "Systems metabolic engineering as an enabling technology in accomplishing sustainable development goals", Microb. Biotechnol., 10(5), 1254-1258 (2017. 9), http://dx.doi.org/10.1111/1751-7915.12766

514. Oh, S.Y., Heo, N.S., Shukla, S., Cho, H.-J., Vilian, A.T.E., Kim, J., Lee, S.Y., Han, Y.-K., Yoo, S.M., and Huh, Y.S. "Development of gold nanoparticle aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat", Sci. Rep. 7: 10130. http://doi.org/10.1038/s41598-017-10188-2

513. Park, S., Kim, C., Lee, D., Song, D.H., Cheon, K.C., Lee, H.S., Kim, S.J., Kim, J.C., and Lee, S.Y. "Construction of Bacillus thuringiensis simulant strains suitable for environmental release", Appl. Environ. Microbiol., 2017:83, 9 e00126-17 (10 August, 2017), http://doi.org/10.1128/AEM.00126-17

512. Jo, Y., Park, S., Jung, J., Yoon, J., Joo, H., Kim, M.-H., Kang, S.-J., Choi, M.C., Lee, S.Y., and Park, Y. "Holographic deep learning for rapid optical screening of anthrax spores", Sci. Adv. 2017;3: e1700606 (4 August 2017), http://doi.org/10.1126/sciadv.1700606

511. Park, S., Kim, C.-H., Jeong, S.T., and Lee, S.Y. "Surrogate strains of human pathogens for field release", Bioengineered, DOI:10.1080/21655979.2017.1349044 (July 2017), http://dx.doi.org/10.1080/21655979.2017.1349044

510. Cho, J.S., Choi, K.R., Prabowo, C.P.S., Shin, J.H., Yang, D., Jang, J., and Lee, S.Y. "CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum", Metab. Eng., 42:157-167 (July 2017), http://dx.doi.org/10.1016/j.ymben.2017.06.010

509. Chae, T.U., Choi, S.Y., Kim, J.W., Ko, Y.S., and Lee, S.Y. "Recent advances in systems metabolic engineering tools and strategies", Curr. Opin. Biotechnol., 47:67–82, DOI: 10.1016/j.copbio.2017.06.007 (October 2017), http://dx.doi.org/10.1016/j.copbio.2017.06.007

508. Blin, K., Wolf, T., Chevrette, M.G., Lu, X., Schwalen, C.J., Kautsar, S.A., Suarez Duran, H.G., de los Santos, E.L.C., Kim, H.U., Nave, M., Dickschat, J.S., Mitchell, D.A., Shelest, E., Breitling, R., Takano, E., Lee, S.Y., Weber, T., and Medema, M.H. "antiSMASH 4.0 - improvements in chemistry prediction and gene cluster boundary identification",Nucleic Acids Res., 45 (W1): W36-W41 (2017. 07), http://dx.doi.org/10.1093/nar/gkx319

507. Jiang, X., Ellabaan, M.M.H., Charusanti, P., Munck, C., Blin, K., Tong, Y., Weber, T., Sommer, M.O.A., & Lee, S.Y. "Dissemination of antibiotic resistance genes from antibiotic producers to pathogens", Nature Comm. 8:15784, DOI: 10.1038/ncomms15784 (Jun 7, 2017), http://doi.org/10.1038/ncomms15784

506. Luo, Z.W. and Lee, S.Y. "Biotransformation of p-xylene into terephthalic acid by engineered Escherichia coli", Nature Comm. 8:15689, DOI: 10.1038/ncomms15689 (May 31, 2017), http://doi.org/10.1038/ncomms15689

505. Chae, T.U., Ko, Y.-S., Hwang, K.-S., and Lee, S.Y. "Metabolic engineering of Escherichia coli for the production of four-, five- and six-carbon lactams", Metab. Eng., 41: 82-91 (2017. 5), http://doi.org/10.1016/j.ymben.2017.04.001

504. Hur, W., Ryu, J.Y., Kim, H.U., Hong, S.W., Lee, E.B., Lee, S.Y., and Yoon, S.K. "Systems approach to characterize the metabolism of liver cancer stem cells expressing CD133", Sci. Rep. 7, 45557; doi: 10.1038/srep45557 (2017. 4), http://dx.doi.org/10.1038/srep45557

503. Kim, W.J., Kim, H.U., and Lee, S.Y., "Current state and applications of microbial genome-scale metabolic models", Curr. Opin. Sys. Biol., 2: 10-18 (2017. 4), http://dx.doi.org/10.1016/j.coisb.2017.03.001

502. Musiol-Kroll, E.M., Zubeil, F., Schafhauser, T., Hartner, T., Kulik, A., McArthur, J., Koryakina, I., Wohlleben, W., Grond, S., Williams, G.J., Lee, S.Y., and Weber, T., "Polyketide bio-derivatization using the promiscuous acyltransferase KirCII", ACS Syn. Biol., 6(3): 421-427 (2017. 3), http://dx.doi.org/10.1021/acssynbio.6b00341

501.Cho, C. and Lee, S.Y. "Efficient gene knockdown in Clostridium acetobutylicum by synthetic small regulatory RNAs", Biotechnol. Bioeng., 114(2):  374-384 (2017. 2), http://dx.doi.org/10.1002/bit.26077

500. Kim, W.J., Ahn, J.H., Kim, H.U., Kim, T.Y., and Lee, S.Y. "Metabolic engineering of Mannheimia succiniciproducens for succinic acid production based on elementary mode analysis with clustering", Biotechnol. J. 12, 1600701, DOI 10.1002/biot.201600701 (2017.2), http://dx.doi.org/10.1002/biot.201600701

499. Blin, K., Medema, M.H., Kottmann, R., Lee, S.Y., and Weber, T. "The antiSMASH database, a comprehensive database of microbial secondary metabolite biosynthetic gene clusters", Nucleic Acids Res., 45 (D1): D555-D559 (2017. 1), http://dx.doi.org/10.1093/nar/gkw960

498. Cho, C., Choe, D., Jang, Y.-S., Kim, K.-J., Kim, W.J., Cho, B.-K., Papoutsakis, E.T., Bennett, G.N., Seung, D.Y., and Lee, S.Y., "Genome analysis of a hyper acetone-butanol-ethanol (ABE) producing Clostridium acetobutylicum BKM19", Biotechnol. J. 12, 1600457, DOI 10.1002/biot.201600457 (2017.2), http://dx.doi.org/10.1002/biot.201600457

497. Yoo, S.M. and Lee, S.Y., "Optical biosensors for the detection of pathogenic microorganisms", Trends Biotechnol., 34(1): 7-25 (2016. 1), http://dx.doi.org/10.1016/j.tibtech.2015.09.012

496. Kang, M., Yoo, S.M, Gwak, R., Eom, G., Kim, J.H., Lee, S.Y., and Kim, B., "Electro-triggering and electrochemical monitoring of dopamine exocytosis from a single cell by ultrathin electrodes based on Au nanowire", Nanoscale, 8: 214-218 (2016. 1), http://dx.doi.org/10.1039/C5NR06021D

495.Kim, E.B., Seo, J.M., Kim, G.W., Lee, S.Y., and Park, T.J. "In vivo synthesis of europium selenide nanoparticles and related cytotoxicity evaluation of human cells", Enzyme Microb. Technol., 95: 201-208 (2016. 12), http://dx.doi.org/10.1016/j.enzmictec.2016.08.012

494. Cheon, S., Kim, H.M., Gustavsson, M., and Lee, S.Y. "Recent trends in metabolic engineering of microorganisms for the production of advanced biofuels", Curr. Opin. Chem. Biol., 35: 10-21 (2016. 12), http://dx.doi.org/10.1016/j.cbpa.2016.08.003

493.Yang, J.E., Kim, J.W., Oh, Y.H., Choi, S.Y., Lee, H., Park, A.-R., Shin, J., Park, S.J., and Lee, S.Y. "Biosynthesis of poly(2-hydroxyisovalerate-co-lactate) by metabolically engineered Escherichia coli", Biotechnol. J. , 11(12): 1572-1585 (2016. 12), http://dx.doi.org/10.1002/biot.201600420

492. Ahn, J.H., Jang, Y.-S., and Lee, S.Y. "Production of succinic acid by metabolically engineered microorganisms", Curr. Opin. Biotechnol., 42: 54-66 (2016. 12), http://dx.doi.org/10.1016/j.copbio.2016.02.034

491.Choi, K.R. and Lee, S.Y. "CRISPR technologies for bacterial systems: current achievements and future directions", Biotechnol. Adv., 34(7): 1180-1209 (2016. 11), http://dx.doi.org/10.1016/j.biotechadv.2016.08.002

490. Lee, J.W., Yi, J., Kim, T.Y., Choi, S., Ahn, J.H., Song, H., Lee, M.-H., and Lee, S.Y. "Homo-succinic acid production by metabolically engineered Mannheimia succiniciproducens", Metab. Eng., 38: 409-417 (2016. 11), http://dx.doi.org/10.1016/j.ymben.2016.10.004

489. Song, C.W., Kim, J.W., Cho, I.J., and Lee, S.Y. "Metabolic engineering of Escherichia coli for the production of 3-hydroxypropionic acid and malonic acid through beta-alanine route", ACS Syn. Biol., 5(11): 1256-1263 (2016. 11), http://dx.doi.org/10.1021/acssynbio.6b00007

488.Choi, S., Kim, H.U., Kim, T.Y., and Lee, S.Y. "Systematic engineering of TCA cycle for optimal production of a four-carbon platform chemical 4-hydroxybutyric acid in Escherichia coli", Metab. Eng., 38: 264-273 (2016. 11), http://dx.doi.org/10.1016/j.ymben.2016.09.004

487.Choi, S., Song, H., Lim, S.W., Kim, T.Y., Ahn, J.H., Lee, J.W., Lee, M.-H., and Lee, S.Y. "Highly selective production of succinic acid by metabolically engineered Mannheimia succiniciproducens and its efficient purification", Biotechnol. Bioeng., 113(10): 2168-2177, (2016. 10), http://dx.doi.org/10.1002/bit.25988

486. Shin, J.H., Park, S.H., Oh, Y.H., Choi, J.W., Lee, M.H., Cho, J.S., Jung, K.J., Joo, J.C., Yu, J., Park, S.J., and Lee, S.Y. "Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid", Microb. Cell Fact.,  15(174): 1-13 (2016. 10), http://dx.doi.org/10.1186/s12934-016-0566-8

485.Gustavsson, M. and Lee, S.Y. "Prospects of microbial cell factories developed through systems metabolic engineering", Microb. Biotechnol., 9(5): 610-617 (2016. 9), http://dx.doi.org/10.1111/1751-7915.12385

484.Sung, M., Yoo, S.M., Jun, R., Lee, J.E., Lee, S.Y., Na, D. "Optimization of Phage λ Promoter Strength for Synthetic Small Regulatory RNA-based Metabolic Engineering", Biotechnol. Bioprocess Eng., 2016, 21(4), 4:483-490. (2016. 8), http://dx.doi.org/10.1007/s12257-016-0245-y

483. Kim, H.U., Charusanti, P., Lee, S.Y., and Weber, T. "Metabolic engineering with systems biology tools to optimize production of prokaryotic secondary metabolites", Nat. Prod. Rep., 33: 933-941 (2016. 7), http://dx.doi.org/10.1039/c6np00019c

482.Lee, J., Jang, Y.-S., Papoutsakis, E.T., and Lee, S.Y. "Stable and enhanced gene expression in Clostridium acetobutylicum using synthetic untranslated regions with a stem-loop", J. Biotechnol., 230(20): 40-43 (2016. 7), http://dx.doi.org/10.1016/j.jbiotec.2016.05.020

481.Shin, J., Lee, S., Go, M.-J., Lee, S.Y., Kim, S.C., Lee, C.-H., Cho, B.-K., "Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing", Sci. Rep. 6(29681): 1-10 (2016. 6), http://dx.doi.org/10.1038/srep29681

480.Lee, J., Jang, Y.-S., Han, M.-J., Kim, J.Y., Lee, S.Y. "Deciphering Clostridium tyrobutyricum Metabolism Based on the Whole-Genome Sequence and Proteome Analyses", mBio, 7(3): e00743-16, doi:10.1128/mBio.00743-16 (2016. 6), http://dx.doi.org/10.1128/mBio.00743-16

479. Blin, K., Pedersen, L.E., Weber, T., and Lee, S.Y., "CRISPy-web: An online resource to design sgRNAs for CRISPR applications in microbes", Synth. Syst. Biotechnol.,  1(2): 118-121 (2016. 6), http://dx.doi.org/10.1016/j.synbio.2016.01.003

478. Yoo, S.M., Baek, Y.-K., Shin, S., Kim, J.-H., Jung, H.-T., Choi, Y.-K. and Lee, S.Y., "Single Walled Carbon Nanotube-Based Electrical Biosensor for the Label-Free Detection of Pathogenic Bacteria" J. Nanosci. Nanotech., 16(6): 6520-6525 (2016. 6), http://dx.doi.org/10.1166/jnn.2016.10882

477. Hyun, M.S., Park, J.P., Seo, D., Chang, S.-J., Lee, S.J., Lee, S.Y., Kwak, K., Park, T.J., "Enzymatic formation of carbohydrate rings catalyzed by single-walled carbon nanotubes", Bioproc. Biosys. Eng., 39(5): 725-733 (2016. 5), http://dx.doi.org/10.1007/s00449-016-1553-5

476. Choi, S.Y., Park, S.J., Kim, W.J., Yang, J.E., Lee, H., Shin, J., and Lee, S.Y. "One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli", Nature Biotechnol., 34(4): 435-440 (2016. 4), http://dx.doi.org/10.1038/nbt.3485

475. Liu, J., Chan, S.H.J., Brock-Nannestad, T., Chen, J., Lee, S.Y., Solem, C., Jensen, P.R. "Combining metabolic engineering and biocompatible chemistry for high-yield production of homo-diacetyl and homo-(S,S)-2,3-butanediol", Metab. Eng., 36: 57-67 (2016. 7), http://dx.doi.org/10.1016/j.ymben.2016.02.008

474. Choi, K.R., Shin, J.H., Cho, J.S., Yang, D., and Lee, S.Y. "Systems metabolic engineering of Escherichia coli", EcoSal Plus 2016, http://dx.doi.org/10.1128/ecosalplus.ESP-0010-2015

473. Kim, J.Y.H., Kwak, H.S., Sung, Y.J., Choi, H.I., Hong, M.E., Lim, H.S., Lee, J.-H., Lee, S.Y., and Sim, S.J., "Microfluidic high-throughput selection of microalgal strains with superior photosynthetic productivity using competitive phototaxis", Sci. Rep., 6(21155): 1-11 (2016. 2), http://dx.doi.org/10.1038/srep21155

472. Chae, C.G., Kim, Y.J., Lee, S.J., Oh, Y.H., Yang, J.E., Joo, J.C., Kang, K.H., Jang, Y.-A., Lee, H., Park, A.-R., Song, B.K., Lee, S.Y., Park, S.J., "Biosynthesis of poly(2-hydroxybutyrate-co-lactate) in metabolically engineered Escherichia coli", Biotechnol. Bioproc. Eng., 21(1): 169-174 (2016. 1), http://dx.doi.org/10.1007/s12257-015-0749-x

471. Park, T.J., Lee, K.G., and Lee, S.Y. "Advances in microbial biosynthesis of metal nanoparticles", Appl. Microbiol. Biotechnol., 100(2): 521-534 (2016. 1), http://dx.doi.org/10.1007/s00253-015-6904-7

470. Moon, H.G., Jang, Y.-S., Cho, C., Lee, J., Binkley, R., and Lee, S.Y., "One hundred years of clostridial butanol fermentation", FEMS Microbiol. Lett., 363(3): 1-15 (2016. 1), http://dx.doi.org/10.1093/femsle/fnw001

469. Gwak, R., Kim, H., Yoo, S.M., Lee, S.Y., Lee, G-.J., Lee, M-.K. Rhee, C-.K., Kang, T., Kim, B. "Precisely determining ultralow level UO22+ in natural water with plasmonic nanowire interstice sensor", Sci. Rep., 6 (19646): 1-7 (2016. 1), http://dx.doi.org/10.1038/srep19646                                                                                                 

2011-2015

468. Weber, T., Charusanti, P., Musiol-Kroll, E.-W., Jiang, X., Tong, Y., Kim, H.U., and Lee, S.Y., "Metabolic engineering of antibiotic factories: new tools for antibiotic production in actinomycetes", Trends Biotechnol., 33(1): 15-26 (2015. 1), http://dx.doi.org/10.1016/j.tibtech.2014.10.009

467. Yoo, S.M., Kim, D.-K., and Lee, S.Y., "Aptamer-functionalized localized surface plasmon resonance sensor for the multiplexed detection of different bacterial species", Talanta, 132: 112-117 (2015. 1), http://dx.doi.org/10.1016/j.talanta.2014.09.003

466. Jang, Y.-S. and Lee, S.Y. "Recent advances in biobutanol production", Industrial Biotechnol., 11(6): 316-321 (2015. 12), http://dx.doi.org/10.1089/ind.2015.0023

465. Chung, H., Yang, J.E., Ha, J.Y., Chae, T.U., Shin, J.H., Gustavsson, M., and Lee, S.Y. "Bio-based production of monomers and polymers by metabolically engineered microorganisms", Curr. Opin. Biotechnol., 36: 73-84 (2015. 12), http://dx.doi.org/10.1016/j.copbio.2015.07.003

464. Cho, C., Choi, S.Y., Luo, Z.W., and Lee, S.Y., "Recent advances in microbial production of fuels and chemicals using tools and strategies of systems metabolic engineering", Biotechnol. Adv., 33(7): 1455–1466 (2015. 11), http://dx.doi.org/10.1016/j.biotechadv.2014.11.006

463. Lakshmanan, M., Kim, T.Y., Chung, B.K.S., Lee, S.Y., and Lee, D.-Y. "Flux-sum analysis identifies metabolite targets for strain improvement", BMC Sys. Biol., 9(73): 1-11 (2015. 10), http://dx.doi.org/10.1186/s12918-015-0198-3

462. Almaas, E., Bauer, E., Bordbar, A., Burgard, A.P., Chang, R.L., Drager, A., Ebrahim, A., Famili, I., Feist, A.M., Fleming, R.M.T., Fong, S.S., Hatzimanikatis, V., Herrgard, M.J., Holder, A., Hucka, M., Hyduke, D., Jamshidi, N., Lee, S.Y., Le Novere, N., Lerman, J.A., Lewis, N.E., Ma, D., Mahadevan, R., Maranas, C., Nagarajan, H., Navid, A., Nielsen, J., Nielsen, L.K., Nogales, J., Noronha, A., Pal, C., Palsson, B.O., Papin, J.A., Patil, K.R., Price, N.D., Reed, J.L., Saunders, M., Senger, R.S., Sonnenschein, N., Sun, Y., and Tiele, I. (alphabetical order), "Do Genome-scale Models Need Exact Solvers or Clearer Standards?", Molecular Systems Biology, 11(10): 831 (2015. 10), http://dx.doi.org/10.15252/msb.20156157

461. Lee, S.Y., Kim, H.U., "Systems strategies for developing industrial microbial strains", Nature Biotechnol., 33(10): 1061–1072 (2015. 10), http://dx.doi.org/10.1038/nbt.3365

460. Song, C.W. and Lee, S.Y. "Combining rational metabolic engineering and flux optimization strategies for efficient production of fumaric acid", Appl. Microbiol. Biotechnol., 99(20): 8455-8464 (2015. 10), http://dx.doi.org/10.1007/s00253-015-6816-6

459. Kim, S., Jang, Y.-S., Ha, S.-C., Ahn, J.-W., Kim, E.-J., Lim, J.H., Cho, C., Ryu, Y.S., Lee, S.K., Lee, S.Y. and Kim, K.-J. "Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum", Nature Communications, 6:8410, DOI: 10.1038/ncomms9410 (2015. 9), http://dx.doi.org/10.1038/ncomms9410

458. Ryu, J.Y., Kim, H.U., and Lee, S.Y., "Human genes with a greater number of transcript variants tend to show biological features of housekeeping and essential genes", Mol. Biosyst., 11: 2798-2807 (2015. 9), http://dx.doi.org/10.1039/c5mb00322a

457. Tong, Y., Charusanti, P., Zhang, L., Weber, T., and Lee, S.Y., "CRISPR-Cas9 based engineering of actinomycetal genomes", ACS Synth. Biol., 4(9): 1020-1029 (2015. 9), http://dx.doi.org/10.1021/acssynbio.5b00038

456. Chae, T.U., Kim, W.J., Choi, S., Park, S.J., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of 1,3-diaminopropane, a three carbon diamine", Sci. Rep. 5(13040): 1-13 (2015. 8), http://dx.doi.org/10.1038/srep13040

455. Ryu, J.Y., Kim, H.U., and Lee, S.Y., "Reconstruction of genome-scale human metabolic models using omics data", Integr. Biol., 7: 859-868 (2015. 8), http://dx.doi.org/10.1039/c5ib00002e

454. Song, C.W., Lee, J., Ko, Y.-S., and Lee, S.Y. "Metabolic engineering of Escherichia coli for the production of 3-aminopropionic acid", Metab. Eng., 30:121-129 (2015.7), http://dx.doi.org/10.1016/j.ymben.2015.05.005

453. Lee, S.Y., Kim, H.M., and Cheon, S., "Metabolic engineering for the production of hydrocarbon fuels", Curr. Opin. Biotechnol., 33: 15-22 (2015. 6), http://dx.doi.org/10.1016/j.copbio.2014.09.008

452. Weber, T., Blin, K., Duddela, S., Krug, D., Kim, H.U., Bruccoleri, R., Lee, S.Y., Fischbach, M.A., Muller, R., Wohlleben, W., Breitling, R., Takano, E., and Medema, M.H., "antiSMASH 3.0 – A comprehensive resource for the genome mining of biosynthetic gene clusters", Nucleic Acids Res., 43(1): W237–W243 (2015. 5), http://dx.doi.org/10.1093/nar/gkv437

451. Yoo, S.M., Kim, D.M., and Lee, S.Y., "Multispot Array Combined with S1 Nuclease-Mediated Elimination of Unpaired Nucleotides", BioChip J., 9(2): 156-163 (2015. 4), http://dx.doi.org/10.1007/s13206-015-9301-5

450. Park, S.J., Lee, S.H., Oh, Y.H., Lee, S.Y., "Establishment of a biosynthesis pathway for (R)-3-hydroxyalkanoates in recombinant Escherichia coli", Korean J. Chem. Eng., 32(4): 702-706 (2015. 4), http://dx.doi.org/10.1007/s11814-014-0240-y

449. Kim, B.S., Kim, G.W., Heo, N.S., Kim, M.S., Yang, K.S., Lee, S.Y., and Park, T.J. "Development of a portable biosensor system for pesticide detection on a metal chip surface integrated with wireless communication", Food Sci. Biotechnol., 24(2): 743-750 (2015. 4), http://dx.doi.org/10.1007/s10068-015-0096-x

448. Oh, Y.H., Lee, S.H., Jang, Y.-A., Choi, J.W. Hong, K.S., Yu, J.H., Shin, J., Mastan, S.G., David, Y., Baylon, M.G., Lee, S.Y., and Park, S.J., "Development of rice bran treatment process in large scale and its use for the synthesis of polyhydroxyalkanoates from rice bran hydrolysate solution", Bioresource Technol., 181:283-290 (2015. 4), http://dx.doi.org/10.1016/j.biortech.2015.01.075

447. Cho, C., Jang, Y.-S., Moon, H.G., Lee, J., and Lee, S.Y., "Metabolic engineering of clostridia for the production of chemicals", Biofuels Bioprod. Bioref., 9(2): 211-225 (2015. 3/4), http://dx.doi.org/10.1002/bbb.1531

446. Kim, H.U., Ryu, J.Y., Lee, J.O. and Lee, S.Y., "A systems approach to traditional oriental medicine", Nature Biotechnol. 33:264-268 (2015. 3), http://dx.doi.org/10.1038/nbt.3167

445. Pronk, J.T., Lee, S.Y., Lievense, J., Pierce, J., Palsson, B., Uhlen, M. and Nielsen, J., "How to set up collaborations between academia and industrial biotech companies", Nature Biotechnol. 33:237-240 (2015. 3) 

444. Choi, S., Song, C.W., Shin, J.H., and Lee, S.Y.., "Biorefineries for the production of top building block chemicals and their derivatives", Metab. Eng., 28: 223-239 (2015.3), http://dx.doi.org/10.1016/j.ymben.2014.12.007

443. Kim, T.Y., Park, J.M., Kim, H.U., Cho, K.M., and Lee, S.Y., "Design of homo-organic acid producing strains using multi-objective optimization", Metab. Eng. 28: 63-73 (2015.3), http://dx.doi.org/10.1016/j.ymben.2014.11.012

442. Park, H.-S., Um, Y., Sim, S.J., Lee, S.Y., and Woo, H. M., "Transcriptomic analysis of Corynebacterium glutamicum in the response to the toxicity of furfural present in lignocellulosic hydrolysates", Process Biochem. 50:347–356 (2015. 3), http://dx.doi.org/10.1016/j.procbio.2014.11.014

441. Park, S.J., Jang, Y.-A., Noh, W., Oh, Y.H., Lee, H., David, Y., Baylon, M.G., Shin, J., Yang, J.E., Choi, S.Y., Lee, S.H., Lee, S.Y., "Metabolic engineering of Ralstonia eutropha for the production of polyhydroxyalkanoates from sucrose", Biotechnol. Bioeng., 112:638–643 (2015. 3), http://dx.doi.org/10.1002/bit.25469

440. Jung, K., Choi, J.-D.-R., Lee, D., Seo, C., Lee, J.W., Lee, S.Y., Chang, H.N., and Kim, Y.-C., "Permeation characteristics of volatile fatty acids solution by forward osmosis", Process Biochem., 50:669-677 (2015. 2), http://dx.doi.org/10.1016/j.procbio.2015.01.016

439. Kim, S.Y., Lee, J., and Lee, S.Y., "Metabolic Engineering of Corynebacterium glutamicum for the Production of L-Ornithine", Biotechnol. Bioeng. 112:416–421 (2015. 2), http://dx.doi.org/10.1002/bit.25440

438. Hwang, H.J., Choi, S.P., Lee, S.Y., Choi, J.-I., Han, S.J., and Lee, P.C., "Dynamics of membrane fatty acid composition of succinic acid-producing Anaerobiospirillum succiniciproducens", J. Biotechnol. 193(10): 130–133 (2015. 1), http://dx.doi.org/10.1016/j.jbiotec.2014.11.028

437. Kim, B., Kim, W.J., Kim, D. I., and Lee, S.Y., "Applications of genome-scale metabolic network model in metabolic engineering", J. Ind. Microbiol. Biotechnol. 42: 339–348 (2015. 1), http://dx.doi.org/10.1007/s10295-014-1554-9

436. Yun, S., Lee, E.-G., Kim, S.-Y., Shin, J.M., Jung, W.S., Oh, D.-B., Lee, S.Y., Kwon, O., "The CpxRA two-component system is involved in the maintenance of the integrity of the cell envelope in the rumen bacterium Mannheimia succiniciproducens", Curr. Microbiol. 70:103-109 (2015. 1), http://dx.doi.org/10.1007/s00284-014-0686-5

435. Song, C.W., Lee, J., and Lee, S.Y., "Genome engineering and gene expression control for bacterial strain development", Biotechnol. J., 10: 48-60 (2015. 1), http://dx.doi.org/10.1002/biot.201400057

434. Hwang, K.-S., Kim, H.U., Charusantia, P., Palsson, B., and Lee, S.Y., "Systems biology and biotechnology of Streptomyces species for the production of secondary metabolites", Biotechnol. Adv., 32(2): 255-268 (2014. 3,4), http://dx.doi.org/10.1016/j.biotechadv.2013.10.008

433. Kim, M.I., Park, T.J., Heo, N.S., Woo, M.-A., Cho, D., Lee, S.Y., and Park, H.G. "Cell-Based Method Utilizing Fluorescent Escherichia coli Auxotrophs for Quantification of Multiple Amino Acids", Anal. Chem., 86(5): 2489-2496 (2014. 3), http://dx.doi.org/10.1021/ac403429s

432. Yang, J.E., Choi, Y.J., Lee, S.J., Kang, K.-H., Lee, H., Oh, Y.H., Lee, S.H., Park, S.J., and Lee, S.Y., "Metabolic engineering of Escherichia coli for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose", Appl. Microbiol. Biotechnol., 98(1): 95-104 (2014.1), http://dx.doi.org/10.1007/s00253-013-5285-z

431. Shin, J.H, Lee, S.Y., "Metabolic engineering of microorganisms for the production of L-arginine and its derivatives", Microb. Cell Fact., 13: 166, doi: 10.1186/s12934-014-0166-4 (2014. 12), http://dx.doi.org/10.1186/s12934-014-0166-4

430. Park, S.J., Oh, Y.H., Noh, W., Kim, H.Y., Shin, J.H., Lee, E.G., Lee, S., David, Y., Baylon, M.G., Song, B.K., Jegal, J., Lee, S.Y., and Lee, S.H., "High-level conversion of L-lysine into 5-aminovalerate that can be used for nylon 6, 5 synthesis", Biotechnol. J., 9(10): 1322-1328 (2014. 10), http://dx.doi.org/10.1002/biot.201400156

429. Choi, Y.J., Lee, J., Jang, Y.-S., and Lee, S.Y., "Metabolic engineering of microorganisms for the production of higher alcohols", mBio, 5(5): e01524-14, doi: 10.1128/mBio.01524-14  (2014. 9), http://dx.doi.org/10.1128/mBio.01524-14

428. Park, S.H., Kim, H.U., Kim, T.Y., Park, J.S., Kim, S.-S. and Lee, S.Y., "Metabolic engineering of Corynebacterium glutamicum for L-arginine production", Nature Commun., 5: 4618, doi: 10.1038/ncomms5618 (2014. 8), http://dx.doi.org/10.1038/ncomms5618

427. Nocon, J., Steiger, M.G., Pfeffer, M. Sohn, S.B., Kim, T.Y., Maurer, M., Rubmayer, H., Pflugl, S., Ask, M., Haberhauer-Troyer, C., Ortmayr, K., Hann, S., Kollensperger, G., Gasser, B., Lee, S.Y., and Mattanovich, D., "Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production", Metab. Eng., 24: 129-138 (2014. 7), http://dx.doi.org/10.1016/j.ymben.2014.05.011

426. Kim, H.U., Kim, B., Seung, D.Y., and Lee, S.Y., "Effects of introducing heterologous pathways on microbial metabolism with respect to metabolic optimality", Biotechnol. Bioprocess Eng., 19(4): 660-667 (2014. 7), http://dx.doi.org/10.1007/s12257-014-0137-y

425. Sohn, M.J., Yoo, S.J., Oh, D.-B., Kwon, O., Lee, S.Y., Sibirny, A.A., and Kang, H.A., "Novel cysteine-centered sulfur metabolic pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha", PLOS One, 9(6): e100725, doi: 10.1371/journal.pone.0100725 (2014. 6), http://dx.doi.org/10.1371/journal.pone.0100725

424. Jang, Y.-S., Han, M.-J., Lee, J., Im, J.A., Lee, Y.H., Papoutsakis, E.T., Bennett, G., and Lee, S.Y. "Proteomic analyses of the phase transition from acidogenesis to solventogenesis using solventogenic and non-solventogenic Clostridium acetobutylicum strains", Appl. Microbiol. Biotechnol., 98(11): 5105-5115 (2014. 6), http://dx.doi.org/10.1007/s00253-014-5738-z

423. Choi, M., Al-Zahrani, S.M., and Lee, S.Y., "Kinetic model-based feed-forward controlled fed-batch fermentation of Lactobacillus rhamnosus for the production of lactic acid from Arabic date juice", Bioproc. Biosys. Eng., 37(6):  1007-1015 (2014. 6), http://dx.doi.org/10.1007/s00449-013-1071-7

422. Kim, B., Park, H., Na, D., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of phenol from glucose", Biotechnol. J., 9(5): 621-629 (2014. 5), http://dx.doi.org/10.1002/biot.201300263

421. Jang, Y.-S., Im, J.A., Choi, S.Y., Lee, J.I., and Lee, S.Y. "Metabolic engineering of Clostridium acetobutylicum for butyric acid production with high butyric acid selectivity", Metab. Eng., 23: 165-174 (2014. 5), http://dx.doi.org/10.1016/j.ymben.2014.03.004

420. Nielsen, J., Fussenegger, M., Keasling, J., Lee, S.Y., Liao, J.C., Prather, K. and Palsson, B., "Engineering synergy in biotechnology", Nature Chem. Biol., 10: 319-322 (2014. 5), http://dx.doi.org/10.1038/nchembio.1519

419. Kim, J.-Y., Ahn, J.-H., Moon, D.-I., Park, T.J., Lee, S.Y. and Choi, Y.-K., "Multiplex electrical detection of avian influenza and human immunodeficiency virus with an underlap-embedded silicon nanowire field-effect transistor", Biosens. Bioelectron., 55(15): 162-167 (2014. 5), http://dx.doi.org/10.1016/j.bios.2013.12.014

418. Park, W.M., Choi, B.G., Huh, Y.S., Hong, W.H., Lee, S.Y., and Park, T.J., "Facile Functionalization of Colloidal Gold Nanorods by the Specific Binding of an Engineered Protein that is Preferred over CTAB Bilayers", ChemPlusChem., 78(1): 48-51 (2013. 1), http://dx.doi.org/10.1002/cplu.201200239

417. Yun, C., Kim, T.Y., Zhang, T., Kim, Y., Lee, S.Y., Park, S., Friedler, F., and Bertok, B. "Determination of the thermodynamically dominant metabolic pathways", Ind. Eng. Chem. Res., 52(1): 222-229 (2013. 1), http://dx.doi.org/10.1021/ie300652h

416. Na, D., Yoo, S.M., Chung, H., Park, H., Park, J.H., and Lee, S.Y., "Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs", Nature Biotechnol., 31(2): 170-174 (2013. 2), http://dx.doi.org/10.1038/nbt.2461

415. Kim, H., Kang, T., Lee, H., Ryoo, H., Yoo, S.M., Lee, S.Y., and Kim,B., "Facile Fabrication of Multi-targeted and Stable Biochemical SERS Sensors", Chem. Asian J., 8(12): 3010-3014 (2013.12), http://dx.doi.org/10.1002/asia.201300764

414. Jang, Y.-S., Woo, H.M., Im, J.A., Kim, I.H., Lee, S.Y., "Metabolic engineering of Clostridium acetobutylicum for enhanced production of butyric acid", Appl. Microbiol. Biotechnol., 97(21): 9355-9363 (2013. 11), http://dx.doi.org/10.1007/s00253-013-5161-x

413. Lee, S.H., Kang, K.-H., Kim, E.Y., Chae, T.U., Oh, Y.H., Hong, S.H., Song, B.K., Jegal, J., Park, S.J., and Lee, S.Y., "Metabolic engineering of Escherichia coli for enhanced biosynthesis of poly(3-hydroxybutyrate) based on proteome analysis", Biotechnol. Lett., 35(10): 1631-1637 (2013. 10), http://dx.doi.org/10.1007/s10529-013-1246-y

412. Yang, J.E., Choi, S.Y., Shin, J.H., Park, S.J. and Lee, S.Y., "Microbial production of lactate-containing polyesters", Microb. Biotechnol., 6(6): 621-636 (2013. 11), http://dx.doi.org/10.1111/1751-7915.12066

411. Kim, H.U. and Lee, S.Y., "Applications of genome-scale metabolic network models in biopharmaceutical industry", Pharm. Bioprocess., 1(4): 337-339 (2013. 10), http://dx.doi.org/10.4155/PBP.13.37

410. Choi, Y.J., Lee, S.Y., "Microbial production of short-chain alkanes", Nature, 502(7472): 571-574 (2013. 10), http://dx.doi.org/10.1038/nature12536

409. Park, T.J., Heo, N.S., Yim, S.S., Park, J.H., Jeong, K.J., and Lee, S.Y., "Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis", Microb. Cell Fact., 12: 81 doi: 10.1186/1475-2859-12-81 (2013. 9), http://dx.doi.org/10.1186/1475-2859-12-81

408. Choi, S., Kim, H.U., Kim, T.Y., Kim, W.J., Lee, M.H., and Lee, S.Y., "Production of 4-hydroxybutyric acid by metabolically engineered Mannheimia succiniciproducens and its conversion to gamma-butyrolactone by acid treatment", Metab. Eng., 20: 73-83 (2013. 11), http://dx.doi.org/10.1016/j.ymben.2013.09.001

407. Yoo, S.M., Kang, M., Kang, T., Kim, D.M., Lee, S.Y. and Kim, B. "Electro-triggered, spatioselective, quantitative gene delivery into a single cell nucleus by Au nanowire nanoinjector", Nano Lett., 13(6): 2431-2435 (2013. 6), http://dx.doi.org/10.1021/nl4003393

406. Park, S.J., Jang, Y.-A., Lee, H., Park, A.-R., Yang, J.E., Shin, J., Oh, Y.H., Song, B.K., Jegal, J., Lee, S.H., and Lee, S.Y., "Metabolic engineering of Ralstonia eutropha for the biosynthesis of 2-hydroxyacid-containing polyhydroxyalkanoates", Metab. Eng., 20: 20-28 (2013. 11), http://dx.doi.org/10.1016/j.ymben.2013.08.002

405. Shin, J.H., Kim, H.U., Kim, D.I., and Lee, S.Y., "Production of bulk chemicals via novel metabolic pathways in microorganisms", Biotechnol. Adv., 31(6): 925-935 (2013. 11), http://dx.doi.org/10.1016/j.biotechadv.2012.12.008

404. Kim, H.U., Kim, W.J., and Lee, S.Y., "Flux-coupled genes and their use in metabolic flux analysis", Biotechnol. J., 8(9): 1035-1042 (2013.9), http://dx.doi.org/10.1002/biot.201200279

403. Jang, Y.S., Malaviya, A., Lee, J., Im, J.A., Lee, S.Y., Lee, J., Eom, M.-H., Cho, J.-H. and Seung, D.Y., "Metabolic Engineering of Clostridium acetobutylicum for the Enhanced Production of Isopropanol-Butanol-Ethanol Fuel Mixture", Biotechnol. Prog., 29(4): 1083-1088 (2013. 7, 8), http://dx.doi.org/10.1002/btpr.1733

402. Yoo, S.M., Na, D., Lee, S.Y., "Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli", Nature Protoc., 8(9): 1694-1707 (2013. 8), http://dx.doi.org/10.1038/nprot.2013.105

401. Lee, J.H., Lee, S.H., Yim, S.S., Kang, K.-H., Lee, S.Y., Park, S.J., and Jeong, K.J. "Quantified high-throughput screening of Escherichia coli producing poly(3-hydroxybutyrate) based on FACS", Appl. Biochem. Biotechnol., 170(7): 1767-1779 (2013. 8), http://dx.doi.org/10.1007/s12010-013-0311-2

400. Song, C.W. and Lee, S.Y., "Rapid one-step inactivation of single or multiple genes in Escherichia coli", Biotechnol. J., 8(7): 776-784 (2013. 7), http://dx.doi.org/10.1002/biot.201300153

399. Song, C.W., Kim, D.I., Choi, S., Jang, J.W., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of fumaric acid", Biotechnol. Bioeng., 110(7): 2025-2034 (2013. 7), http://dx.doi.org/10.1002/bit.24868

398. Jang, Y.-S., Malaviya, A., and Lee, S.Y. "Acetone-Butanol-Ethanol Production with High Productivity using Clostridium acetobutylicum BKM19", Biotechnol. Bioeng., 110(6): 1646-1653 (2013. 6), http://dx.doi.org/10.1002/bit.24843

397. Park, S.J., Kang, K.-H., Lee, H., Park, A.-R., Yang, J.E., Oh, Y.H., Song, B.K., Jegal, J., Lee, S.H., Lee, S.Y., "Propionyl-CoA dependent biosynthesis of 2-hydroxybutyrate containing polyhydroxyalkanoates in metabolically engineered Escherichia coli", J. Biotechnol., 165(2): 93-98 (2013. 5), http://dx.doi.org/10.1016/j.jbiotec.2013.03.005

396. Choi, J.-D.-R., Jang, Y.S., Cho, J.-H., Seung, D., Lee, S.Y., Papoutsakis, E.T., Bennett, G.N., Song, H.,  "Characterization and Evaluation of Corn Steep Liquid in Acetone- Butanol-Ethanol Production by Clostridium acetobutylicum", Biotechnol. Bioprocess Eng., 18(2): 266-271 (2013. 4), http://dx.doi.org/10.1007/s12257-012-0619-8

395. Ahn, J.-H., Im, M., Park, T.J., Lee, S.Y., and Choi, Y.-K., "Label-free and real-time detection of avian influenza using nanowire field effect transistors", J. Biomed, Nanotechnol., 9(3): 1-4 (2013. 3), http://dx.doi.org/10.1166/jbn.2013.1501

394. Park, S.J., Kim, E.Y., Noh, W., Park, H.M., Oh, Y.H., Lee, S.H., Song, B.K., Jegal, J., and Lee, S.Y. "Metabolic engineering of Escherichia coli for the production of 5-aminovalerate and glutarate as C5 platform chemicals", Metab. Eng., 16: 42-47 (2013. 3), http://dx.doi.org/10.1016/j.ymben.2012.11.011

393. Kim, J.-Y., Choi, K., Moon, D.-I., Ahn, J.-H., Park, T.J., Lee, S.Y. and Choi, Y.-K., "Surface engineering for enhancement of sensitivity in an underlap-FET biosensor by control of wettability", Biosens. Bioelectron., 41: 867-870 (2013. 3), http://dx.doi.org/10.1016/j.bios.2012.08.036

392. Kim, J.R., Kim, S.H., Lee, S.Y., Lee, P.C., "Construction of homologous and heterologous synthetic sucrose utilizing modules and their application for carotenoid production in recombinant Escherichia coli", Bioresour. Technol., 130: 288-295 (2013. 2), http://dx.doi.org/10.1016/j.biortech.2012.11.148

391. Jang, Y.-S., Lee, J., Malaviya, A., Seung, D.Y., Cho, J.H., and Lee, S.Y., "Butanol production from renewable biomass: rediscovery of metabolic pathways and metabolic engineering", Biotechnol. J., 7(2): 186-198 (2012. 2), http://dx.doi.org/10.1002/biot.201100059

390. Park, T.J., Lee, S.J., Kim, D.-K., Heo, N.S., Park, J.Y., and Lee, S.Y., "Development of label-free optical diagnosis for sensitive detection of influenza virus with genetically engineered fusion protein", Talanta 89: 246-252(2012. 1), http://dx.doi.org/10.1016/j.talanta.2011.12.021

389. Park, S.J., Lee, T.W., Lim, S.-C., Kim, T.W., Lee, H., Kim, M.K., Lee, S.H., Song, B.K., and Lee, S.Y., "Biosynthesis of polyhydroxyalkanoates containing 2-hydroxybutyrate from unrelated carbon source by metabolically engineered Escherichia coli", Appl. Microbiol. Biotechnol. 93(1): 273-283 (2012. 1), http://dx.doi.org/10.1007/s00253-011-3530-x

388. Lee, S.Y., Mattanovich, D., and Villaverde, A. "Systems metabolic engineering, industrial biotechnology and microbial cell factories", Microb. Cell Fact., 11: 156, doi: 10.1186/1475-2859-11-156 (2012. 12), http://dx.doi.org/10.1186/1475-2859-11-156

387. Choi, S.J., Lee, J., Jang, Y.S., Park, J.H., Lee, S.Y., and Kim, I.H., "Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum", J. Microbiol., 50(6): 1063-1066 (2012.12), http://dx.doi.org/10.1007/s12275-012-2373-1

386. Chung, H., Kim, T.Y., and Lee, S.Y., "Recent advances in production of recombinant spider silk proteins", Curr. Opin. Biotechnol., 23(6): 957-964 (2012.12), http://dx.doi.org/10.1016/j.copbio.2012.03.013

385. Park, J.H., Oh, J.E., Lee, K.H., Kim, J.Y., and Lee, S.Y., "Rational Design of Escherichia coli for L-isoleucine Production", ACS Synth. Biol., 1(11): 532-540 (2012.11), http://dx.doi.org/10.1021/sb300071a

384. Park, S.J., Kim, T.W., Kim, M.K., and Lee, S.Y., Lim, S.-C., "Advanced bacterial polyhydroxyalkanoates: towards a versatile and sustainable platform for unnatural tailor-made polyesters", Biotechnol. Adv., 30(6): 1196-1206 (2012. 11), http://dx.doi.org/10.1016/j.biotechadv.2011.11.007

383. Jang, Y.-S., Lee, J.Y., Lee, J., Park, J.H., Im, J.A., Eom, M.-H., Lee, J., Lee, S.-H., Song, H., Cho, J.-H., Seung, D.Y., and Lee, S.Y., "Enhanced Butanol Production Obtained by Reinforcing the Direct Butanol-Forming Route in Clostridium acetobutylicum", mBio, 3(5): e00314-12, doi: 10.1128/mBio.00314-12 (2012. 10), http://dx.doi.org/10.1128/mBio.00314-12

382. Jang, Y.-S., Malaviya, A., Cho, C., Lee, J., and Lee, S.Y., "Butanol production from renewable biomass by clostridia", Bioresour. Technol., 123: 653-663 (2012. 11), http://dx.doi.org/10.1016/j.biortech.2012.07.104

381. Park, J.M., Park, H.M., Kim, W.J., Kim, H.U., Kim, T.Y., and Lee, S.Y., "Flux variability scanning based on enforced objective flux for identifying gene amplification targets", BMC Syst. Biol., 6:106, doi: 10.1186/1752-0509-6-106 (2012. 8), http://dx.doi.org/10.1186/1752-0509-6-106

380. Yun, S., Shin, J.M., Kim, O.-C., Jung, Y.R., Oh, D.-B., Lee, S.Y. and Kwon, O., "Probing the ArcA Regulon in the Rumen Bacterium Mannheimia succiniciproducens by Genome-Wide Expression Profiling", J. Microbiol., 50(4): 665-672 (2012. 8), http://dx.doi.org/10.1007/s12275-012-2007-7

379. Lee, K.G., Hong, J., Wang, K.W., Heo, N.S., Kim, D.H., Lee, S.Y., Lee, S.J., and Park, T.J., "In vitro biosynthesis of metal nanoparticles in microdroplets", ACS Nano, 6(8): 6998-7008 (2012. 8), http://dx.doi.org/10.1021/nn302043q

378. Choi, Y.J., Park, J.H., Kim, T.Y., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of 1-propanol", Metab. Eng., 14(5): 477-486 (2012. 9), http://dx.doi.org/10.1016/j.ymben.2012.07.006

377. Ahn, J.-H., Kim, J.-Y., Choi, K., Kim, D.-I., Kim, C.-H., Park, T.J., Lee, S.Y., and Choi, Y.-K., "Nanowire FET biosensors on a bulk silicon substrate", IEEE Trans. Electron Devvices, 59(8): 2243-2249 (2012. 8), http://dx.doi.org/10.1109/TED.2012.2200105

376. Kang, T., Yoo, S.M., Kang, M., Lee, H., Kim, H., Lee, S.Y., and Kim, B. "Single-Step Multiplex Detection of Toxic Metal Ions by Au Nanowires-on-Chip Sensor Using Reporter Elimination", Lab Chip, 12: 3077-3081 (2012. 5), http://dx.doi.org/10.1039/c2lc40185a

375. Sohn, S.B., Kim, T.Y., Lee, J.H., and Lee, S.Y. "Genome-scale metabolic model of the fission yeast Schizosaccharomyces pombe and the reconciliation of in silico/in vivo mutant growth", BMC Syst. Biol., 6: 49, doi: 10.1186/1752-0509-6-49 (2012. 5), http://dx.doi.org/10.1186/1752-0509-6-49

374. Han, M.-J., Lee, S.Y., and Hong, S.H., "Comparative analysis of envelope proteomes in Escherichia coli B and K-12 strains", J. Microbiol. Biotechnol., 22(4): 470-478 (2012. 4), http://dx.doi.org/10.4014/jmb.1110.10080

373. Kim, H.-J., Kwon, Y.D., Lee, S.Y., and Kim, P. "An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production", Appl. Microbiol. Biotechnol., 94(4): 1079-1086 (2012. 5), http://dx.doi.org/10.1007/s00253-011-3779-0

372. Lee, E.-G., Kim, S., Oh, D.-B., Lee, S.Y., and Kwon, O., "The Distinct Roles of β-Galactosidase Paralogues of the Rumen Bacterium Mannheimia succiniciproducens", J. Bacteriol., 194(2): 426-436 (2012. 1), http://dx.doi.org/10.1128/JB.05911-11

371. Kim, T.Y., Sohn, S.B., Kim, Y.B., Kim, W.J., and Lee, S.Y., "Recent advances in reconstruction and applications of genome-scale metabolic models", Curr. Opin. Biotechnol., 23(4): 617-623 (2012. 8), http://dx.doi.org/10.1016/j.copbio.2011.10.007

370. Jang, Y.-S., Kim, B., Shin, J.H., Choi, Y.J., Choi, S., Song, C.W., Lee, J., Park, H.G., Lee, S.Y. "Bio-based production of C2-C6 platform chemicals", Biotechnol. Bioeng., 109(10): 2437-2459 (2012. 10), http://dx.doi.org/10.1002/bit.24599

369. Jang, Y.-S., Park, J.M., Choi, S., Choi, Y.J., Seung, D.Y., Cho, J.H., and Lee, S.Y., "Engineering of microorganisms for the production of biofuels and perspectives based on systems metabolic engineering approaches", Biotechnol. Adv., 30(5): 989-1000 (2012. 9), http://dx.doi.org/10.1016/j.biotechadv.2011.08.015

368. Heo, N.S., Zheng, S., Yang, M., Lee, S.J., Lee, S.Y., Kim, H.-J., Lee, C.-S., Park, T.J.,  "Label-Free Electrochemical Diagnosis of Viral Antigen with Genetically Engineered Fusion Protein", Sensors, 12(8): 10097-10108 (2012. 8), http://dx.doi.org/10.3390/s120810097

367. Lee, J.W., Na, D., Park, J.M., Lee, J., Choi, S., and Lee, S.Y. "Systems metabolic engineering of microorganisms for natural and non-natural chemicals", Nature Chem. Biol. 8(6): 536-546 (2012. 6), http://dx.doi.org/10.1038/nchembio.970

366. Park, C., Yun, S., Lee, S.Y., Park, K., Lee, J., "Metabolic Profiling of Klebsiella oxytoca: Evaluation of Methods for Extraction of Intracellular Metabolites Using UPLC/Q-TOF-MS", Appl. Biochem. Biotechnol., 167(3): 425-438 (2012. 6), http://dx.doi.org//10.1007/s12010-012-9685-9

365. Jung, J.H., Park, T.J., Lee, S.Y., and Seo, T.S., "Homogeneous biogenic paramagnetic nanoparticle synthesis based on a microfluidic droplet generator", Angew. Chem., 51(23): 5634-5637 (2012. 6), http://dx.doi.org/10.1002/anie.201108977

364. Yoon, S.H., Han, M.-J., Jeong, H., Lee, C.H., Xia, X.-X., Lee, D.-H., Shim, J.H., Lee, S.Y., Oh, T.K., and Kim, J. F. "Comparative multi-omics systems analysis of Escherichia coli strains B and K-12", Genome Biol.,13: R37, doi: 10.1186/gb-2012-13-5-r37 (2012. 5), http://dx.doi.org/10.1186/gb-2012-13-5-r37

363. Mingshou, L., Lee, S., Kim, B., Park, C., Oh, M., Park, K., Lee, S.Y., and Lee, J., "Identification of Factors Regulating Escherichia coli 2,3-Butanediol Production by Continuous Culture and Metabolic Flux Analysis", J. Microbiol. Biotechnol., 22(5): 659-667 (2012. 5), http://dx.doi.org/10.4014/jmb.1112.12018

362. Park, S.J., Kim, M.K., and Lee, S.Y., "One-step fermentative production of polylactic acid (PLA)", Chem. Eng. Prog., 108(5): 51-55 (2012. 5)

361. Park, S.J., Kim, M.K., Lee, S.Y., "Microbial metabolic engineering for the production of polyesters", Biotech international, 24: 11-13 (2012. 3) 

360. Seol, M.-L., Choi, S.-J., Baek, D.J., Park, T.J., Ahn, J.-H., Lee, S.Y. and Choi, Y.-K., "A nanoforest structure for practical surface-enhanced Raman scattering substrates", Nanotechnology., 23(9): 1-7, (2012. 3), http://dx.doi.org/10.1088/0957-4484/23/9/095301

359. Kim, H.U., Sohn, S.B., and Lee, S.Y., "Metabolic network modeling and simulation for drug targeting and discovery", Biotechnol. J., 7(3): 330-342 (2012. 3), http://dx.doi.org/10.1002/biot.201100159

358. Kim, J.-Y., Ahn, J.-H., Lee, K.-W., Choi, S.-J., Im, M., Kim, S., Kim, C.-H., Park, T.J., Lee, S.Y., and Choi, Y.-K., "An underlap channel-embedded field-effect transistor for biosensor application in watery and dry environment", IEEE Trans. Nanotechnol., 11(2): 390-394 (2012. 3), http://dx.doi.org/10.1109/TNANO.2011.2175006

357. Kim, J.-Y., Ahn, J.-H., Moon, D.-I., Kim, S., Park, T.J., Lee, S.Y. and Choi, Y.-K.,  "A dual-gate field-effect transistor for label-free electrical detection of avian influenza", BioNanoScience, 2(1):35-41 (2012. 3), http://dx.doi.org/10.1007/s12668-011-0035-0

356. Lee, J., Jang, Y.-S., Choi, S.J., Im, J.A., Song, H., Cho, J.H., Seung, D.Y., Papoutsakis, E.T., Bennett, G.N., and Lee, S.Y., "Metabolic engineering of Clostridium acetobutylicum ATCC 824 for isopropanol-butanol-ethanol fermentation", Appl. Environ. Microbiol., 78(5): 1416-1423 (2012. 3), http://dx.doi.org/10.1128/AEM.06382-11                                  

355. Malaviya, A., Jang, Y.-S., and Lee, S.Y., "Continuous butanol production with reduced byproducts formation from glycerol by a hyper producing mutant of Clostridium pasteurianum", Appl. Microbiol. Biotechnol., 93(4): 1485-1494 (2012. 2), https://doi.org/10.1007/s00253-011-3629-0

354. Park, S.J., Lee, S.Y., Kim, T.W., Jung, Y.K., and Yang, T.H., "Biosynthesis of lactate containing polyesters by metabolically engineered bacteria", Biotechnol. J., 7(2): 199-212 (2012. 2), https://doi.org/10.1002/biot.201100070

353. Kwon, Y.-D., Kim, S., Lee, S.Y., and Kim, P. "Long-term continuous adaptation of Escherichia coli to high succinate stress and transcriptome analysis of the tolerant strain", J. Biosci. Bioeng. 111(1)26-30 (2011. 1), https://doi.org/10.1016/j.jbiosc.2010.08.007

352. Qian, Z.-G., Xia, X.-X., and Lee, S.Y. "Metabolic engineering of Escherichia coli for the production of cadaverine: a five carbon diamine", Biotechnol. Bioeng. 108(1): 93-103 (2011. 1), https://doi.org/10.1002/bit.22918

351 Jung, Y.K. and Lee, S.Y. "Efficient production of polylactic acid and its copolymers by metabolically engineered Escherichia coli", J. Biotechnol. 151(1): 94-101 (2011. 1), https://doi.org/10.1016/j.jbiotec.2010.11.009

350. Lee, S.Y., Sohn, S.B., Park, J.M., Lee, J.W., "Biorefinery Research in Korea"., Asia Pacific Biotech News, 15(12): 15-21 (2011. 12)

349. Kim, H.U., Kim, T.Y., and Lee, S.Y., "Framework for network modularization and Bayesian network analysis to investigate the perturbed metabolic network", BMC Syst. Biol., 5 (Suppl 2): S14, doi: 10.1186/1752-0509-5-S2-S14 (2011.12.04), https://doi.org/10.1186/1752-0509-5-s2-s14

348. Lee, J.W., Kim, H.U., Choi, S., Yi, J., and Lee, S.Y. "Microbial production of building block chemicals and polymers", Curr. Opin. Biotechnol., 22(6): 758-767 (2011.12), https://doi.org/10.1016/j.copbio.2011.02.011

347. Yoo, S.M., Kang, T., Lee, S.Y., and Kim, B., "Combining Nanowire SERRS Sensor and Target Recycling Reaction for Ultrasensitive and Multiplex Identification of Pathogenic Fungi" Small, 7(23): 3371-3376 (2011. 12), https://doi.org/10.1002/smll.201100633

346. Lee, S., Kim, J., Park, T. J., Shin, Y., Lee, S.Y., Han, Y.-M., Kang, S., and Park, H.-S., "The effects of the physical properties of culture substrates on the growth and differentiation of human embryonic stem cells", Biomaterials, 32(34): 8816-8829 (2011. 12), https://doi.org/10.1016/j.biomaterials.2011.07.058

345. Ravikumar, S., Yoo, I.K., Lee, S.Y., Hong, S.H., "Construction of Copper Removing Bacteria Through the Integration of Two-Component System and Cell Surface Display", Appl. Biochem. Biotechnol., 165(7-8): 1674-1681 (2011. 12), https://doi.org/10.1007/s12010-011-9386-9

344. Ravikumar, S., Yoo, I.K., Lee, S.Y., Hong, S.H., "A study on the dynamics of the zraP gene expression profile and its application to the construction of zinc adsorption bacteria", Bioproc. Biosys. Eng., 34(9): 1119-1126 (2011. 11), https://doi.org/10.1007/s00449-011-0562-7

343. Ahn, J.-H., Choi, S.-J., Han, J.-W., Park, T.J., Lee, S.Y., and Choi, Y.-K., "Investigation of size dependence on sensitivity for nanowire FET biosensors", IEEE Transactions on Nanotechnology, 10(6): 1405-1411 (2011. 11), https://doi.org/10.1109/TNANO.2011.2157519

342. Park, T.J., Lee, S.J., Pan, J.-G., Jung, H.-C., Park, J.Y., Park, J.P., Lee, S.Y., "DNA capturing machinery through spore-displayed proteins", Lett. Appl. Microbiol. 53(4): 445-451 (2011. 10), https://doi.org/10.1111/j.1472-765x.2011.03131.x

341. Kim, D.-K., Yoo, S.M., Park, T.J., Yoshikawa, H., Tamiya, E., Park, J.Y., and Lee, S.Y., "Plasmonic properties of multispot copper-capped nanoparticle array chip and its application to optical biosensor for pathogen detection of multiplex DNAs", Anal. Chem., 83(16): 6215-6222 (2011. 8), https://doi.org/10.1021/ac2007762

340. Park, J.M., Kim, T.Y., and Lee, S.Y. "Genome-scale reconstruction and in silico analysis of the Ralstonia eutropha H16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production", BMC Sys. Biol., 5:101, doi: :10.1186/1752-0509-5-101 (2011. 6), https://doi.org/10.1186/1752-0509-5-101

339. Choi, B.G., Yang, M., Park, T.J., Huh, Y.S., Lee, S.Y., Hong, W.H. and Park, H., "Programmable peptide-directed two dimentional arrays of various nanoparticles on grapheme sheets", Nanoscale, 3(8): 3208-3213 (2011. 8), https://doi.org/10.1039/c1nr10276a

338. Yim, H., Haselbeck, R., Niu, W., Pujol-Baxley, C., Burgard, A., Boldt, J., Khandurina, J., Trawick, J.D., Osterhout, R.E., Stephen, R., Estadilla, J., Teisan, S., Schreyer, H.B., Andrae, S., Yang, T.H., Lee, S.Y., Burk, M.J., and Van Dien, S. "Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol", Nature Chem. Biol., 7(7): 445-452 (2011. 7), https://doi.org/10.1038/nchembio.580

337. Yoo, S.M., Kang, T., Kim, B., and Lee, S.Y. "Detection of Single Nucleotide Polymorphism by Au Nanowire-on-Film SERS Sensor Coupled with S1 Nuclease Treatment", Chem. Eur. J., 17(31): 8657-8662 (2011. 7), https://doi.org/10.1002/chem.201003372

336. Lee, J.W., Kim, T.Y., Jang, Y.S., Choi, S., and Lee, S.Y. "Systems metabolic engineering for chemicals and materials", Trends Biotechnol. 29(8): 370-378 (2011. 8), https://doi.org/10.1016/j.tibtech.2011.04.001

335. Yang, M., Choi, B.G., Park, T.J., Heo, N.S., Hong, W.H., and Lee, S.Y., "Site-specific immobilization of gold binding polypeptide on gold nanoparticle-coated graphene sheet for biosensor application", Nanoscale, 7(3): 2950-2956 (2011. 6), https://doi.org/10.1039/c1nr10197h

334. Liu, F., Choi, K.S., Park, T.J., Lee, S.Y., Seo, T.S., "Graphene-based electrochemical biosensor for pathogenic virus detection", BioChip J., 5 (2): 123-128 (2011. 6), https://doi.org/10.1007/s13206-011-5204-2

333. Baek, Y.K., Jung, D-.H., Yoo, S.M., Shin, S.H.R., Kim, J.H., Choi, Y.K., Lee, S.Y. and Jung, H.-T., "Label-Free Detection of DNA Hybridization Using Pyrene-Functionalized Single-Walled Carbon Nanotubes: Effect of Chemical Structures of Pyrene Molecules on DNA Sensing Performance", J. Nanosci. Nanotechnol. 11(5): 4210-4216 (2011. 5), https://doi.org/10.1166/jnn.2011.3663

332. Chang, H.N., Kim, N.-J., Kang, J., Jeong, C.M., Choi, J., Fei, Q., Kim, B.J., Kwon, S., Lee, S.Y. and Kim, J. "Multi-stage high cell continuous fermentation for high productivity and titre", Bioproc. Biosys. Eng. 34(4): 419-431 (2011. 5), https://doi.org/10.1007/s00449-010-0485-8

331. Park, T.J., Park, J.P., Lee, S.J., Jung, D.-H., Ko, Y.K., Jung, H.-T., and Lee, S.Y., "Alignment of SWNTs by protein-ligand interaction of functionalized magnetic particles under low magnetic fields", J. Nanosci. Nanotechnol. 11(5): 4540-4545 (2011. 5), https://doi.org/10.1166/jnn.2011.3668

330. Kim, D.-K., Park, T.J., Tamiya, E., and Lee, S.Y., "Label-free detection of leptin antibody-antigen interaction by using LSPR-based optical biosensor", J. Nanosci. Nanotechnol. 11(5): 4188-4193 (2011. 5), https://doi.org/10.1166/jnn.2011.3680

329. Park, J.H., Jang, Y.-S., Lee, J.W., and Lee, S.Y. "Escherichia coli W as a new platform strain for the enhanced production of L-valine by systems metabolic engineering", Biotechnol. Bioeng. 108(5):1140-1147 (2011. 5), https://doi.org/10.1002/bit.23044

328. Han, M.-J., Yun, H., Lee, J.W., Lee, Y.H., Lee, S.Y., Yoo, J.-S., Kim, J.Y., Kim, J.F., and Hur, C.-G. "Genome-wide identification of the subcellular localization of the Escherichia coli B proteome using experimental and computational methods", Proteomics, 11(7): 1213-1227 (2011. 4), https://doi.org/10.1002/pmic.201000191

327. Yang, T.H., Jung, Y.K., Kang, H.O., Kim, T.W., Park, S.J., and Lee, S.Y. "Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli", Appl. Microbiol. Biotechnol. 90(2): 603-614 (2011. 4), https://doi.org/10.1007/s00253-010-3077-2

326. Park, J.H., Kim, T.Y., Lee, K.H., and Lee, S.Y., "Fed-batch culture of Escherichia coli for L-valine production based on in silico flux response analysis", Biotechnol. Bioeng. 108(4): 934-946 (2011. 4), https://doi.org/10.1002/bit.22995

325. Lee, S.Y. "Metabolic engineering, systems biology and synthetic biology (p.120-121, in Crystal Ball 2011)", Microbial Biotechnol. 4(2): 109-137 (2011. 3), https://doi.org/10.1111/j.1567-1364.2011.00783.x

324. Han, J.-I., Choi, H.-K., Lee, S.-W., Orwin, P.M., Kim, J., LaRoe, S.L., Kim, T.-G., O’Neil, J., Leadbetter, J.R., Lee, S.Y., Hur, C.-G., Spain, J.C., Ovchinnikova, G., Goodwin, L., Han, C., "Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110", J. Bacteriol. 193(5): 1183-1190 (2011. 3), https://doi.org/10.1128/jb.00925-10

323. Park, C., Park, C., Lee, Y.-J., Lee, S.Y., Oh, H.B., and Lee, J., "Determination of the Intracellular Concentrations of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry", Bull. Korean Chem. Soc., 32(2): 524-530 (2011. 2), http://dx.doi.org/10.5012/bkcs.2011.32.2.524

322. Kang, T., Yoo, S.M., Yoon, I., Lee, S., Choo, J., Lee, S.Y., and Kim, B. "Au Nanowire-on-Film SERRS Sensor for Ultrasensitive Hg2+ Detection", Chem.- A European J., 17(7): 2211-2214 (2011. 2), https://doi.org/10.1002/chem.201001663

321. Oh, E., Lu, M., Park, C., Park, C., Oh, H.B., Lee, S.Y., Lee J., "Dynamic Modeling of Lactic Acid Fermentation Metabolism with Lactococcus lactis", J. Microbiol. Biotechnol., 21(2): 162-169 (2011. 2), https://doi.org/10.4014/jmb.1007.07066

320. Park, T.J, Park, J.P., Lee, S.J. and Lee, S.Y., "Quantitative studies of carbohydrate-protein interaction by using functionalized bacterial spores in solution and on chip", Biotechnol. Bioprocess Eng., 16(1): 190-195 (2011. 2), https://doi.org/10.1007/s12257-010-0063-6

319. Yang, M., Choi, B.G., Park, H., Park, T.J., Hong, W.H., and Lee, S.Y., "Directed self-assembly of gold nanoparticles on graphene-ionic liquid hybrid for enhancing electrocatalytic activity", Electroanalysis, 23(4): 850-857 (2011. 2), https://doi.org/10.1002/elan.201000645

318. Han, M.-J., Lee, J.W., and Lee, S.Y., "Understanding and engineering of microbial cells based on proteomics and its conjunction with other omics studies", Proteomics, 11(4): 721-743 (2011. 2), https://doi.org/10.1002/pmic.201000411

317. Im, M., Ahn, J.-H., Han, J.-W., Park, T.J., Lee, S.Y., and Choi, Y.-K., "Development of a point-of-care testing platform with a nanogap-embedded separated double-gate field effect transistor array and its readout system for detection of avian influenza", IEEE Sens. J., 11(2): 351-360 (2011. 2), https://doi.org/10.1109/JSEN.2010.2062502

316. Archer, C.T., Kim, J.F., Jeong, H., Park, J.H., Vickers, C.E., Lee, S.Y., and Nielsen, L.K. "The genome sequence of E. coli W ATCC 9637: comparative genome analysis and an improved genome-scale reconstruction of E. coli", BMC Genomics, 12(9): 1-20 (2011. 1), https://doi.org/10.1186/1471-2164-12-9

315. Xia, X. X., Qian, Z., and Lee, S.Y., "Comparative proteomic and genetic analyses reveal unidentified mutations in Escherichia coli XL1-Blue and DH5alpha", FEMS Microbiol. Lett. 314(2): 119-124 (2011. 1), https://doi.org/10.1111/j.1574-6968.2010.02157.x

314. Kim, H.U., Kim, S.Y., Jeong, H., Kim, T.Y., Kim, J.J., Choy, H.E., Yi, K.Y., Rhee, J.H., Lee, S.Y., "Integrative genome-scale metabolic analysis of Vibrio vulnificus for drug targeting and discovery", Molecular Systems Biology, 7(460): 1-15 (2011. 1), https://doi.org/10.1038/msb.2010.115

313. Park, T.J., Lee, S.-K., Yoo, S.M., Yang, S.-M., and Lee, S.Y., "Development of reflective biosensor using fabrication of functionalized photonic nanocrystals", J. Nanosci. Nanotechnol., 11(1): 632-637 (2011. 1), https://doi.org/10.1166/jnn.2011.3269

312. Won, W., Park, C., Park, C., Lee, S.Y., Lee, K.S., and Lee, J., "Parameter Estimation and Dynamic Control Analysis of Central Carbon Metabolism in Escherichia coli", Biotechnol. Bioproc. Eng., 16(2): 216-228 (2011.4), http://dx.doi.org/10.1007/s12257-010-0238-1

311. Kim, N.-J., Choi, J.H., Kim, Y.C., Lee, J., Lee, S.Y., Chan, H.N., Lee, P.C., "Development of anaerobically inducible nar promoter expression vectors for the expression of recombinant proteins in Escherichia coli", J. Biotechnol., 151(1): 102-107 (2011.1), https://doi.org/10.1016/j.jbiotec.2010.11.010

310. Park, T.J., Lee, S.J., Park, J.P., Yang, M.H., Choi, J.H., and Lee, S.Y. "Characterization of a Bacterial self-assembly surface layer protein and its application as an electrical nanobiosensor", J. Nanosci. Nanotechnol. 11(1): 402-407 (2011. 1), https://doi.org/10.1166/jnn.2011.3264

2001-2010

309. Lee, J.W. and Lee, S.Y., "Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7", Bioprocess Bioprocess Biosyst. Eng. 33(1): 97-107 (2010. 1), http://dx.doi.org/10.1007/s00449-009-0339-4

308. Baek, Y.-K., Yoo, S.M., Kang, T., Jeon, H.-J., Kim, K., Lee, J.-S., Lee, S.Y., Kim, B., and Jung, H.-T. "Large-scale highly ordered chitosan-core-Au-shell nanopatterns with plasmonic tunability : A top-down approach to fabricate core-shell nanostructure", Adv. Funct. Materials, 20(24): 4273-4278 (2010. 12), http://dx.doi.org/10.1002/adfm.201001051

307. Oh, Y.H., Oh, I.J., Jung, C., Lee, S.Y., Lee, J., "The Effect of Protectants and pH Changes on the Cellular Growth and Succinic Acid Yield of Mannheimia succiniciproducens LPK7", J. Microbiol. Biotechnol. 20(12): 1677-1680 (2010. 12), http://dx.doi.org/10.4014/jmb.1003.03032

306. Choi, B.G., Park, H., Yang, M.H., Jung, Y.M., Lee, S.Y., Hong, W.H., and Park, T.J., "Microwave-assisted synthesis of highly water-soluble graphene towards electrical DNA sensor", Nanoscale, 12(2): 2692-2697 (2010.12), http://dx.doi.org/10.1039/c0nr00562b

305. Lee, K.Y., Park, J.M., Kim, T.Y., Yun, H., and Lee, S.Y. "The genome-scale metabolic network analysis of Zymomonas mobilis ZM4 explains physiological features and suggests ethanol and succinic acid production strategies", Microb. Cell Fact., 9:94, doi:10.1186/1475-2859-9-94 (2010.11), http://dx.doi.org/10.1186/1475-2859-9-94

304. Lee, J.W., Choi, S., Park, J.H., Vickers, C., Nielsen, L..K., and Lee, S.Y. "Development of sucrose-utilizing Escherichia coli K-12 strain by cloning β-fructofuranosidases and its application for L-threonine production", Appl. Microbiol. Biotechnol. 88(4): 905-913 (2010. 10), http://dx.doi.org/10.1007/s00253-010-2825-7

303. Park, H., Park, T.J., Huh, Y.S., Choi, B.G., Ko, S., Lee, S.Y., and Hong, W.H., "Immobilization of genetically-engineered fusion proteins on gold-decorated carbon nanotube hybrid films for the fabrication of biosensor platforms", J. Colloid Interf. Sci., 350(2): 453-458 (2010.10), http://dx.doi.org/10.1016/j.jcis.2010.06.058

302. Park, T.J., Lee, S.Y., Heo, N.S., and Seo, T.S., "In vivo synthesis of diverse metal nanoparticles by recombinant Escherichia coli", Angew. Chem. Int. Ed., 49(39): 7019-7024 (2010.9)

301. Han, M.-J., Kim, N.-J., Lee, S.Y., and Chang, H.N. "Extracellular proteome of Aspergillus terreus grown on different carbon sources", Curr. Genetics, 56(4): 369-382 (2010. 8), http://dx.doi.org/10.1016/j.jcis.2010.06.058

300. Xia, X.-X., Qian, Z.-G., Ki, C.S., Park, Y.H., Kaplan, D.L., and Lee, S.Y., "Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber" Proc. Nat. Acad. Sci. (PNAS) 107(32): 14059-14063 (2010. 8), http://dx.doi.org/10.1073/pnas.1003366107

299. Park, J.M., Jang, Y.-S., Kim, T.Y., and Lee, S.Y. "Development of a gene knockout system for Ralstonia eutropha H16 based on the broad-host-range vector expressing a mobile group II intron", FEMS Microbiol. Lett., 309(2): 193-200 (2010.8), http://dx.doi.org/10.1111/j.1574-6968.2010.02041.x

298. Park, J.M., Kim, T.Y., and Lee, S.Y. "Prediction of metabolic fluxes by incorporating genomic context and flux-converging pattern analyses", Proc. Nat. Acad. Sci. (PNAS), 107(33): 14931-14936 (2010.8), http://dx.doi.org/10.1073/pnas.1003740107

297. Kim, S., Kim, J.-Y., Ahn, J.-H., Park, T.J., Lee, S.Y., and Choi, Y.-K., "A charge pumping technique to identify biomolecular charge polarity using a nanogap embedded bio-transistor," Appl. Phys. Lett., 97(7): 073702-1-3 (2010.8) - Specially selected in Virtual Journal of Nanoscale Science & Technology, Vol. 22, Issue 9, p.76., http://dx.doi.org/10.1063/1.3473819

296. Ahn, J.-H., Choi, S.-J., Han, J.-W., Park, T.J., Lee, S.Y., Choi, Y.-K., "Double-Gate Nanowire Field Effect Transistor for a Biosensor", Nano Letters, 10(8): 2934-2938 (2010.8), http://dx.doi.org/10.1021/nl1010965

295. Lee, K., Wi, R., Imran, M., Park, T.J., Lee, J., Lee, S.Y., Kim, D.H., " Functionalization Effects of Single-Walled Carbon Nanotubes as Templates for the Synthesis of Silica Nanorods and Study of Growing Mechanism of Silica", ACS Nano., 4(7): 3933-3942 (2010.7), http://dx.doi.org/10.1021/nn100807r

294. Zheng, S., Kim, D.-K., Park, T.J., Lee, S.J., and Lee, S.Y., "Label-free optical diagnosis of hepatitis B virus with genetically engineered fusion proteins", Talanta, 82(2): 803-809 (2010. 7), http://dx.doi.org/10.1016/j.talanta.2010.05.059

293. Sohn, S.B., Kim, T.Y., Park, J.M., and Lee, S.Y. "In silico genome-scale metabolic analysis of Pseudomonas putida KT2440 for polyhydroxyalkanoate synthesis, degradation of aromatics, and anaerobic survival", Biotechnol. J. 5(7): 739-750 (2010.7), http://dx.doi.org/10.1002/biot.201000124

292. Sohn, S.B., Graf, A.B., Kim, T.Y., Gasser, B., Maurer, M., Ferrer, P., Mattanovich, D., and Lee, S.Y. "Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production", Biotechnol. J. 5(7): 705-715 (2010.7), http://dx.doi.org/10.1002/biot.201000078

291. Kim,S., Ahn, J.-H., Park, T.J., Lee, S.Y., Choi, Y.-K., "Comprehensive study of a detection mechanism and optimization strategies to improve sensitivity in a nanogap-embedded biotransistor", J. Appl. Phys., 107(11): 114705-1-5 (2010.6)- Specially selected in Virtual Journal of Biological Physics Research, Vol. 19, Issue 12, p.188., http://dx.doi.org/10.1063/1.3443580

290. Na, D., Kim, T.Y., and Lee, S.Y., "Construction and optimization of synthetic pathways in metabolic engineering", Curr. Opin. Microbiol. 13(3): 363-370 (2010.6), http://dx.doi.org/10.1016/j.mib.2010.02.004

289. Yang, M.H., Choi, B.G., Park, H., Hong, W.H., Lee, S.Y., and Park, T.J. "Development of the glucose biosensor using advanced electrode modified by nanohybrid composing chemically modified grapheme and ionic liquid", Electroanalysis, 22(11): 1223-1228 (2010.6), http://dx.doi.org/10.1002/elan.200900556

288. Park, J.H., and Lee, S.Y. "Metabolic pathways and fermentative production of L-aspartate family amino acids", Biotechnol. J. 5(6): 560-577 (2010.6), http://dx.doi.org/10.1002/biot.201000032

287. Choi, H.S., Lee, S.Y., Kim, T.Y., and Woo, H.M. "In silico identification of gene amplification targets for improving lycopene production", Appl. Environ. Microbiol. 76(10): 3097-3105 (2010.5), http://dx.doi.org/10.1128/AEM.00115-10

286. Lee, P.C., Lee, S.Y., and Chang, H.N. "Kinetic study on succnic acid and acetic acid formations during continuous cultures of Anaerobiospirillum succiniciproducens grown on glycerol", Bioproc. Biosys. Eng. 33(4): 465-471 (2010.5), http://dx.doi.org/10.1007/s00449-009-0355-4

285. Kim, J., Kong, M.K., Lee, S.Y., and Lee, P.C. "Carbon sources-dependent carotenoid production in metabolically engineered Escherichia coli", World J. Microbiol. Biotechnol. 26: 1-9 (2010. 4), http://dx.doi.org/10.1007/s11274-010-0408-5

284. Choi, B.G., Park, H., Park, T.J., Yang, M.H., Kim, J.S., Jang, S.-Y., Heo, N.S., Lee, S.Y., Kong, J. and Hong, W.H., "Solution chemistry of self-assembled graphene nanohybrids for high performance flexible biosensors", ACS Nano, 4(5): 2910-2918 (2010.4), http://dx.doi.org/10.1021/nn100145x

283. Noh, J.-H., Kim, S.-H., Lee, H.-N., Lee, S.Y., and Kim, E.-S. "Isolation and genetic manipulation of the antibiotic down-regulatory gene, wblA ortholog for doxorubicin-producing Streptomyces strain improvement", Appl. Microbiol. Biotechnol. 86(4): 1145-1153 (2010.4), http://dx.doi.org/10.1007/s00253-009-2391-z

282. Kang, T., Yoo, S.M., Yoon, I., Lee, S.Y., and Kim, B. "Patterned multiplex pathogen DNA detection by Au particle-on-wire SERS sensor", Nano Lett. 10(4): 1189-1193 (2010.4), http://dx.doi.org/10.1021/nl1000086

281. Jung, D.-H., Kim, B.H., Lim, Y.T., Kim, J., Lee, S.Y., and Jung, H.-T. "Fabrication of single-walled carbon nanotubes dotted with gold-nano crystals as nanocarriers for DNA delivery", Carbon, 48(4): 1070-1078 (2010.4), http://dx.doi.org/10.1016/j.carbon.2009.11.028

280. Yoo, S.M. and Lee, S.Y., "DNA microarray for the identification of pathogens causing bloodstream infections", Expert Reviews of Molecular Diagnostics, 10(3): 263-268 (2010. 4) Journal cover paper (cover)

279. Cho, A., Yun, H., Park, J.H., Lee, S.Y., and Park, S. "Prediction of novel synthetic pathways for the production of desired chemicals", BMC Syst. Biol. 4:35, doi: 10.1186/1752-0509-4-35 (2010. 3), http://dx.doi.org/10.1186/1752-0509-4-35

278. Kim, T.Y., Kim, H.U., and Lee, S.Y., "Data integration and analysis of biological networks", Curr. Opin. Biotechnol. 21: 78-84 (2010.2) Journal cover paper (cover), http://dx.doi.org/10.1016/j.copbio.2010.01.003

277. Lee, J.W., Choi, S., Kim, J.M., and Lee, S.Y., "Mannheimia succiniciproducens Phosphotransferase System for Sucrose Utilization", Appl. Environ. Microbiol. 76(5): 1699-1703 (2010.3), http://dx.doi.org/10.1128/AEM.02468-09

276. Kim, T.Y., Kim, H.U., and Lee, S.Y., "Metabolite-centric approaches for the discovery of antibacterials using genome-scale metabolic networks", Metab. Eng., 12(2): 105-111 (2010.3), http://dx.doi.org/10.1016/j.ymben.2009.05.004

275. Lee, S.Y. and Park, J.H. "Integration of systems biology with bioprocess engineering: L-threonine production by systems metabolic engineering of Escherichia coli", Adv. Biochem. Eng. Biotechnol. 120:1-19 (2010.2), http://dx.doi.org/10.1007/10_2009_57

274. Kim, S., Ahn, J.-H., Park, T.J., Lee, S.Y., and Choi, Y.-K., "Charge pumping technique to analyze the effect of intrinsically retained charges and extrinsically trapped charges in biomolecules by use of a nanogap embedded bio-transistor", Appl. Phys. Lett., 96(5): 053702, 1-3 (2010.2.) - Specially selected in Virtual Journal of Nanoscale Science & Technology, Vol. 21, Issue 7, p.98 - Specially selected in Virtual Journal of Biological Physics Research, Vol. 19, Issue 4, p.99, http://dx.doi.org/10.1063/1.3300838

273. Han, M.-J., Lee, S.Y., Koh, S.-T., Noh, S.-G., and Han, W.H. "Biotechnological applications of microbial proteomes", J. Biotechnol. 145(4): 341-349 (2010.2)., http://dx.doi.org/10.1016/j.jbiotec.2009.12.018

272. Yoo, S.Y., Kim, D.K., Park, T.J., Kim, E.K., Tamiya, E., and Lee, S.Y., "Detection of most common corneal dystrophies caused by BIGH3 gene point mutations using multi-spot gold-capped nanoparticle array chip", Anal. Chem. 82(4): 1349-1357 (2010.2.), http://dx.doi.org/10.1021/ac902410z

271. Baek, J.H., Han, M.-J., Lee, S.H., and Lee, S.Y. "Enhanced Display of Lipase on the Cell Surface of Escherichia coli Based on Transcriptome Analysis", Appl. Environ. Microbiol. 76(3): 971-973 (2010. 2.), http://dx.doi.org/10.1128/AEM.02463-09

270. Huh, Y.S., Jeong, C.-M., Chang, H.N., Lee, S.Y., Hong, W.H., and Park, T.J., "Rapid separation of bacteriorhodopsin using a laminar-flow extraction system in a microfluidic device", Biomicrofluidics, 4(1): 014103, 1-10 (2010.1.)- Specially selected in Virtual Journal of Biological Physics Research, Vol. 19, Issue 3, p.157, http://dx.doi.org/10.1063/1.3298608

269. Kim, H.U., Kim, T.Y., and Lee, S.Y., "Genome-scale metabolic network analysis and drug targeting of multi-drug resistant pathogen Acinetobacter baumannii AYE", Mol. Biosyst., 6: 339-348 (2010. 2.).Journal cover paper (cover), http://dx.doi.org/10.1039/b916446d

268. Lee, K.-W., Choi, S.-J., Ahn, J.-H., Moon, D.-I., Park, T. J., Lee, S.Y. and Choi, Y.-K., "An underlap field-effect transistor for electrical detection of influenza", Appl. Phys. Lett., 96(3): 033703, 1-3 (2010.1.) - Specially selected in Virtual Journal of Biological Physics Research, Vol. 19, Issue 3, p.156, http://dx.doi.org/10.1063/1.3291617

267. Park, J.H. and Lee, S.Y., "Fermentative production of branched chain amino acids: a focus on metabolic engineering", Appl. Microbiol. Biotechnol. 85(3): 491-506 (2010.1), http://dx.doi.org/10.1007/s00253-009-2307-y

266. Jung, Y.K., Kim, T.Y., Park, S.J., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers", Biotechnol. Bioeng., 105(1): 161- 171 (2010.1). Press Release, About the Author, About this Journal (CNN.com, CNN Tech, CNN Asia, Economist,  U.S. News & World Report, Chosun.com, ETN news), http://dx.doi.org/10.1002/bit.22548

265. Yang, T.H., Kim, T.W., Kang, H.O., Lee, S.-H., Lee, E.J., Lim, S.-C., Oh, S.O., Song, A.-J., Park, S.J., and Lee, S.Y., "Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase", Biotechnol. Bioeng.  105(1): 150-160 (2010.1), http://dx.doi.org/10.1002/bit.22547

 264. Yoo, S.M., Choi, J.Y., Yun, J.K., Choi, J.K., Shin, S.Y., Lee, K., Kim, J.M., and Lee, S.Y., "DNA microarray-based identification of bacterial and fungal pathogens in bloodstream infections", Mol. Cell. Probes, 24(1): 44-52 (2010.1), http://dx.doi.org/10.1016/j.jbiotec.2010.11.010

1992-2000

263. Lee, S.Y., Kim, H.U., Park, J.H., Park, J.M., and Kim, T.Y. "Metabolic engineering of microorganisms: general strategies and drug production", Drug Discovery Today, 14(1/2): 78-88 (2009. 1), http://dx.doi.org/10.1016/j.drudis.2008.08.004

262. Thuy Vu An, N., Hong, S.H. and Lee, S.Y., "Elucidation of Multifaceted Evolutionary Processes of Microorganisms by Comparative Genome-Based Analysis", J. Microbiol. Biotechnol. 19(11): 1301-1305 (2009. 11), http://dx.doi.org/10.4014/jmb.0905.05017

261. Kim, M.I., Kim, N.J., Shang, L., Chang, Y.K., Lee, S.Y. and Chang, H.N., "Continuous Production of Succinic Acid Using an External Membrane Cell Recycle System", J. Microbiol. Biotechnol. 19(11): 1369-1373 (2009. 11), http://dx.doi.org/10.4014/jmb.0903.03034

260. Lee, P.C., Lee, S.Y., and Chang, H.N., "Kinetic study of organic acid formations by Anaerobiospirillum succiniciproducens during continuous cultures", J. Microbiol. Biotechnol. 19(11): 1379-1384 (2009. 11), http://dx.doi.org/10.4014/jmb.0905.05026

259. Kim, T.Y., Kim, H.U., Song, H. and Lee, S.Y., "In silico analysis of the effects of H2 and CO2 on the metabolism of a capnophilic bacterium Mannheimia succiniciproducens", J. Biotechnol., 144(3): 184-189 (2009. 11), http://dx.doi.org/10.1016/j.jbiotec.2009.06.003

258. Qian, Z.-G., Xia, X.-X., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of putrescine, a four carbon diamine", Biotechnol. Bioeng. 104(4): 651-662 (2009. 11), http://dx.doi.org/10.1002/bit.22502

257. Park, J.M., Kim, T.Y., and Lee, S.Y., "Constraints-based genome-scale metabolic simulation for systems metabolic engineering", Biotechnol. Adv. 27(6): 978-988 (2009. 11), http://dx.doi.org/10.1016/j.biotechadv.2009.05.019

256. Baek, Y.-K., Yoo, S.-M., Kim, J.-H., Jung, D.-H., Choi, Y.-K., Kim, Y.S., Lee, S.Y. and Jung, H.-T., "The Effect of Network Density on the DNA-Sensing Performance of Single-Walled Carbon Nanotubes", J. Phys. Chem. C, 113(52): 21566-21571 (2009. 10), http://dx.doi.org/10.1021/jp9080564

255. Lee, J.Y., Jang, Y.-S., Lee, J., Papoutsakis, E.T., and Lee, S.Y., "Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production", Biotechnol. J. 4(10): 1432-1440 (2009.10), http://dx.doi.org/10.1002/biot.200900142

254. Baek, J.H., Han, M.-J., Lee, S.Y., and Yoo J.-S. "Transcriptome and proteome analyses of adaptive responses to methyl methanesulfonate in Escherichia coli K-12 and ada mutant strains", BMC Microbiol. 9:186, doi: 10.1186/1471-2180-9-186 (2009. 9), http://dx.doi.org/10.1186/1471-2180-9-186

253. Oh, Y.-G., Lee, D.-Y., Lee, S.Y., and Park, S. "Multiobjective flux balancing using the NISE method for metabolic network analysis", Biotechnol. Prog., 25(4): 999-1008 (2009. 9), http://dx.doi.org/10.1002/btpr.193

252. Gu, B., Park, T.J., Ahn, J.-H., Huang, X.-J., Lee, S.Y., Choi., Y.-K., "Nanogap field-effect transistor biosensor for electrical detection of avian influenza", Small, 5(17): 2407-2412 (2009. 9), http://dx.doi.org/10.1002/smll.200900450

251. Yoo, S.M., Choi, J.H., Lee, S.Y., and Yoo, N.C. "Applications of DNA microarray in disease diagnostics", J. Microbiol. Biotechnol. 19(7): 635-646 (2009. 7), http://dx.doi.org/10.4014/jmb.0803.226

250. Park, T. J., Hyun, M. S., Lee, H. J., Lee, S. Y., and Ko. S., "A self-assembled fusion protein-based surface plasmon resonance biosensor for rapid diagnosis of severe acute respiratory syndrome", Talanta, 79(2): 295-301 (2009. 7), http://dx.doi.org/10.1016/j.talanta.2009.03.051

249. Kim, S., Ahn, J.-H., Park, T.J., Lee, S.Y., and Choi, Y.-K., "A biomolecular detection method based on charge pumping in a nanogap embedded field-effect-transistor biosensor", Appl. Phys. Lett., 94: 243903-1-3 (2009. 6), http://dx.doi.org/10.1063/1.3148340

248. Park, T.J., Choi, S.-K., Jung, H.-C., Lee, S.Y. and Pan, J.-G., "Spore display using Bacillus thuringiensis exosporium protein InhA", J. Microbiol. Biotechnol., 19(5): 495-501 (2009. 5), http://dx.doi.org/10.4014/jmb.0802.163

247. Kim, J.-H., Kang, T., Yoo, S.M., Lee, S.Y., Kim, B., and Choi, Y.-K., "A well-ordered flower-like gold nanostructure for integrated sensors via surface-enhanced Raman scattering", Nanotechnology, 20(23): 1-6 (2009. 6), http://dx.doi.org/10.1088/0957-4484/20/23/235302

246. Yoo, S.M., Lee, S.Y., Chang, K.H., Yoo, S.Y., Yoo, N.C., Keum, K.C., Yoo, W.M., Kim, J.M., and Choi, J.Y. "High-throughput identification of clinically important bacterial pathogens using DNA microarray", Mol. Cell. Probes, 23(3-4): 171-177 (2009. 6), http://dx.doi.org/10.1016/j.mcp.2009.03.004

245. Park, T.J., Shun, Z., Kang, Y.J., and Lee, S.Y., "Development of whole cell biosensor by cell surface display of gold binding polypeptide on the gold surface", FEMS Microbiol. Lett., 293(1):141-147 (2009. 4), http://dx.doi.org/10.1111/j.1574-6968.2009.01525.x

244. Yang, K. S., Clementz, P., Park, T. J., Lee, S. J., Park, J. P., Kim, D. H. and Lee, S. Y., "Free-flow isoelectric focusing microfluidic device with glass coating by sol-gel methods", Curr. Appl. Phys., 9(2): e66-e70 (2009. 3), http://dx.doi.org/10.1016/j.cap.2008.12.032

243. Lee, H.-N., Im, J.-H., Lee, M.-J., Lee, S.Y., and Kim, E.-S., "A putative secreted solute binding protein, SCO6569 is a possible AfsR2-dependent down-regulator of actinorhodin biosynthesis in Streptomyces coelicolor", Proc. Biochem., 44(3): 373-377 (2009. 3), http://dx.doi.org/10.1016/j.procbio.2008.12.002

242. Park, T.J., Yoo, S.M., Keum, K.C., and Lee, S.Y. "Microarray of DNA-protein complexes on poly-3-hydroxybutyrate surface for pathogen detection", Anal. Bioanal. Chem., 393: 1639-1647 (2009. 3), http://dx.doi.org/10.1007/s00216-008-2574-y

241. Choi, B.G., Park, H., Park, T.J., Kim, D.H., Lee, S.Y., and Hong, W.H. "Development of the electrochemical biosensor for organophosphate chemicals using CNT/ionic liquid bucky gel electrode", Electrochem. Comm., 11(3): 672-675 (2009. 3), http://dx.doi.org/10.1016/j.elecom.2009.01.006

240. Yoo, S.Y., Lee, S.Y. and Yoo, N.C. "Cytokine expression and cancer detection", Med. Sci. Monit. 15(3): RA49-56 (2009. 3)

239. Duong, C.T.P., Lee, H.-N., Choi, S.-S., Lee, S.Y., and Kim, E.-S. "Functional expression of SAV3818, a putative TetR-family transcriptional regulatory gene from Streptomyces avermitilis, stimulates antibiotic production in Streptomyces species", J. Microbiol. Biotechnol. 19(2): 136-139 (2009. 2), http://dx.doi.org/10.4014/jmb.0806.387

238. Oh, I.J., Kim, D.H., Oh, E.K., Lee, S.Y., and Lee, J.W "Optimization and Scale-Up of Succinic Acid Production by Mannheimia succiniciproducens LPK7", J. Microbiol. Biotechnol., 19(2), 167-171 (2009. 2), http://dx.doi.org/10.4014/jmb.0807.447

237. Selvarasu, S., Ow, D.S-W., Lee, S.Y., Lee, M.M., Oh, S.K-W., Karimi, I.A., and Lee, D.-Y. "Characterizing Escherichia coli DH5alpha growth and metabolism in a complex medium using genome-scale flux analysis", Biotechnol. Bioeng., 102(3): 923-934 (2009. 2), http://dx.doi.org/10.1002/bit.22119

236. Han, M.-J., Lee, J.W., Lee, S.Y. and Yoo, J. "Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production", J. Biomedicine Biotechnol., 2008: 1-12 (2008. 1.), http://dx.doi.org/10.1155/2008/735101

235. Kim, K.-H., Kim, J.-H., Huang, X.-J., Yoo, S.M., Lee, S.Y., and Choi, Y.-K. "Doping free nanoscale complementary carbon nanotube field effect transistors with DNA-templated molecular lithography", Small 4(11): 1959-1963 (2008. 11), http://dx.doi.org/10.1002/smll.200800226

234. Lee, P.C., Lee, S.Y., and Chang, H.N. "Succinic acid production by Anaerobiospirillum succiniciproducens ATCC 29305 growing on galactose, galactose/glucose, and galactose/lactose", J. Microbiol. Biotechnol. 18(11): 1792-1796 (2008. 11), http://dx.doi.org/10.4014/jmb.0800.129

233. Choi, J., Yang, K.-W., Lee, T.-Y., and Lee, S.Y. "New time-scale criteria for model simplification of bio-reaction systems", BMC Bioinformatics, 9(338): 1-8 (2008. 8), http://dx.doi.org/10.1186/1471-2105-9-338

232. Han, M.-J., Yun H., and Lee, S.Y. "Microbial small heat shock proteins and their use in biotechnology", Biotechnol. Adv.26(6): 591-609 (2008. 11), http://dx.doi.org/10.1016/j.biotechadv.2008.08.004

231. Lee, J., Yun, H., Feist, A.M., Palsson, B.O., and Lee, S.Y. "Genome-scale reconstruction and in silico analysis of the Clostridium acetobutylicum ATCC 824 metabolic network", Appl. Microbiol. Biotechnol. 80(5): 849-862 (2008. 10), http://dx.doi.org/10.1007/s00253-008-1654-4

230. Park, J.H. and Lee, S.Y. "Towards systems metabolic engineering of microorganisms for amino acid production", Curr. Opin. Biotechnol. 19(5): 454-460 (2008. 10), http://dx.doi.org/10.1016/j.copbio.2008.08.007

229. Park, T. J., Huh, Y. S., Lee, E. Z., Lee, S. J., Zheng, S., Yang, K. S., Lee, S. Y., Hong, W. H., Kim, D. H. and Ko, S., "An active mixing microfluidic system for the colorimetric analysis of intracellular enzyme", Biochip J., 2(1): 33-38 (2008. 3)

228. Huh, Y.S., Park, T.J., Lee, E.Z., Hong, Y.K., Lee, S.Y. and Hong, W.H., "Advanced cleanup process of the free-flow microfluidic device for protein analysis", Ultramicroscopy, 108(10):1365-1370 (2008. 9), http://dx.doi.org/10.1016/j.ultramic.2008.04.085

227. Lee, S.Y., Park, J.H., Jang, S.H., Nielsen, L.K., Kim, J., and Jung, K. S. "Fermentative butanol production by clostridia", Biotechnol. Bioeng., 101(2), 209-228 (2008. 10.), http://dx.doi.org/10.1002/bit.22003

226. Qian, Z.G., Xia, X.X., Choi, J.H., and Lee, S.Y. "Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli", Biotechnol. Bioeng., 101(3), 587-601 (2008. 10.), http://dx.doi.org/10.1002/bit.21898

225. Lee, P.C., Lee, S.Y., and Chang, H.N., "Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens using an internal membrane filtration system", J. Microbiol. Biotechnol., 18(7), 1252-1256 (2008. 7.)

224. Huh, Y.S., Park, T.J., Lee, E.Z., Hong, W.H., and Lee, S.Y., "Development of a fully integrated microfluidic system for sensing infectious viral disease", Electrophoresis, 29(14), 2960-2969 (2008. 7.), http://dx.doi.org/10.1002/elps.200700823

223. Park, J.H., Lee, S.Y., Kim, T.Y., and Kim, H.U., "Application of systems biology for bioprocess development", Trends Biotechnol., 26(8), 404-412 (2008. 8.), http://dx.doi.org/10.1016/j.tibtech.2008.05.001

222. Kwon, Y.D., Lee, S.Y., and Kim, P. "A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase", Biosci. Biotechnol. Biochem., 72(4), 1138-1141 (2008. 7.), http://dx.doi.org/10.1271/bbb.70831

221. Yoo, S.M. and Lee, S.Y., "Diagnosis of Pathogens Using DNA Microarray", Recent Patents on Biotechnology, 2(2): 124-129 (2008. 7.)

220. Lee, S.-H., Park, S.J., and Lee, S.Y., Hong, S.H., "Biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli", Appl. Microbiol. Biotechnol. 79(4): 633-641 (2008. 6.), http://dx.doi.org/10.1007/s00253-008-1473-7

219. Moon, S.Y., Hong, S.H., Kim, T.Y., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of malic acid", Biochem. Eng. J. 40(2): 312-320 (2008. 1.), http://dx.doi.org/10.1016/j.bej.2008.01.001

218. Oh, I.J., Lee, H.W., Park, C.H., Lee, S.Y., and Lee, J.W. "Succinic acid production by continuous fermentation process using Mannheimia succiniciproducens LPK7", J. Microbiol. Biotechnol. 18(5): 908-912 (2008. 5.)

217. Lee, S.Y., Kim, J.M., Song, H., Lee, J.W., Kim, T.Y., and Jang, Y.-S., "From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens", Appl. Microbiol. Biotechnol. 79(1): 11-22 (2008. 5.), http://dx.doi.org/10.1007/s00253-008-1424-3

216. Song, H., Kim, T.Y., Choi, B.-K., Choi, S.J., Nielsen, L., Chang, H.N., and Lee, S.Y., "Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence", Appl. Microbiol. Biotechnol. 79(2): 263-272 (2008. 5.), http://dx.doi.org/10.1007/s00253-008-1425-2

215. Jung, W. S., Jung, Y. R., Oh, D.-B., Kang, H. A., Lee, S. Y., Chavez-C., M., Georgellis, D., Kwon, O., "Characterization of the Arc two-component signal transduction systemof the capnophilic rumen bacterium Mannheimia succiniciproducens", FEMS Microbiol. Lett. 284(1): 109-119 (2008. 7.), http://dx.doi.org/10.1111/j.1574-6968.2008.01187.x

214. Xia, X.-X., Han, M.-J., Lee, S.Y., and Yoo, J.-S., "Comparison of the extracellular proteomes of Escherichia coli B and K-12 strains during high cell density cultivation", Proteomics, 8(1): 2089-2103  (2008. 5.), http://dx.doi.org/10.1002/pmic.200700826

213. Kim, T.Y., Sohn, S.B., Kim, H.U., and Lee, S.Y., "Strategies for systems-level metabolic engineering", Biotechnol. J. 3:612-623 (2008. 5.), http://dx.doi.org/10.1002/biot.200700240

212. Hyeon, B.-H., Kim, H.-Y., Lee, C.M., Moon, S.-H., Kim, E.

-S., and Lee, S.Y. "Bio-Vision 2016: The second national framework plan for biotechnology promotion in Korea", Biotechnol. J. 3:591-600 (2008. 5.), http://dx.doi.org/10.1002/biot.200700248

211. Song, H., Jang, S.H., Park, J.M. and Lee, S.Y., "Modeling of batch fermentation kinetics for succinic acid production by Mannheimia succiniciproducens", Biochem. Eng. J. 40(1): 107-115 (2008. 5.), http://dx.doi.org/10.1016/j.bej.2007.11.021

210. Lee, E. Z., Huh, Y. S., Won, H. J., Hong, Y. K., Park, T. J., Lee, S.Y. and Hong, W. H., "Removal of bovine serum albumin using solid-phase extraction with in-situ polymerized stationary phase in a microfluidic device" J. Chromatogr. A, 1187: 11-17 (2008. 4.), http://dx.doi.org/10.1016/j.chroma.2008.01.084

209. Ganesan, R., Yoo, S.Y., Choi, J.H., Lee, S.Y. and Kim, J.-B., "Simple micropatterning of biomolecules on a diazoketo functionalized photoresist", J. Mater. Chem. 18(6): 703-709 (2008. 1.), http://dx.doi.org/10.1039/b709774c

208. Kim, H.U., Kim, T.Y., and Lee, S.Y., "Metabolic flux analysis and metabolic engineering of microorganisms", Mol. Biosyst. 4: 113-120 (2008. 2.), http://dx.doi.org/10.1039/b712395g

207. Kim, J.M., Lee, K.H. and Lee, S.Y., "Development of a markerless gene knock-out system for Mannheimia succiniciproducens using a temperature-sensitive plasmid", FEMS Microbiol. Lett. 278: 78-85 (2008. 1.), http://dx.doi.org/10.1111/j.1574-6968.2007.00981.x

206. Yoo, S.Y., Kim, T.-I., Lee, S.Y., Kim, E.K., Keum, K.C., Yoo, N.C., and Yoo, W.M. "Development of DNA chip for the diagnosis of most common corneal dystrophies caused by mutations in the bigh3 gene", British J. Opththalmol. 91: 722-727 (2007. 6.), http://dx.doi.org/10.1136/bjo.2006.111070

205. Jun, Y.S., Lee, E.Z., Huh, Y.S., Hong, Y.K., Hong, W.H., Lee, S.Y., "Kinetic study for the extraction of succinic acid with TOA in fermentation broth; effects of pH, salt and contaminated acid", Biochem. Eng. J. 36:8-13 (2007. 8.), http://dx.doi.org/10.1016/j.bej.2006.06.011

204. Jun, Y.S., Huh, Y.S., Park., H.S., Arne, T., Jeon, S.J., Lee, E.Z., Won, H.J., Hong, W.H., Lee, S.Y. and Hong, Y.K., "Adsorption of Pyruvic and Succinic Acid by Amine-Functionalized SBA-15 for the Purification of Succinice Acid from Fermentation Broth", J. Phys. Chem.C, 111, 13076-13086 (2007. 12.), http://dx.doi.org/10.1021/jp072606g

203. Park, J.-N., Sohn, M. J., Oh, D.-B., Kwon, O., Rhee, S. K., Hur, C.-G., Lee, S. Y., Gellissen, G. and Kang, H. A., "Identification of the cadmium-inducible Hansenula polymorpha SEO1 gene promoter by transcriptome analysis and Its application to whole-cell heavy-metal detection systems" Appl. Environ. Microbiol. 73(19): 5990-6000 (2007. 10.), http://dx.doi.org/10.1128/AEM.00863-07

202. Lee, K.H., Park, J.H., Kim, T.Y., Kim, H.U., and Lee, S.Y., "Systems metabolic engineering of Escherichia coli for L-threonine production", Molecular Systems Biology, 3(149): 1-8 (2007. 12.), http://dx.doi.org/10.1038/msb4100196

201. Baek, J.H., Kang, Y.J., and Lee, S.Y., "Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli", FEMS Microbiol. Lett. 277: 254-259 (2007. 12.), http://dx.doi.org/10.1111/j.1574-6968.2007.00965.x

200. Huh, Y.S., Choi, J.H., Park, T.J., Hong, Y.K., Hong, W.H., and Lee, S.Y., "Microfluidic cell disruption system employing a magnetically actuated diaphragm", Electrophoresis, 28: 4748-4757 (2007. 12.), http://dx.doi.org/10.1002/elps.200700366

199. Kim, P.J., Lee, D.Y., Kim, T.Y., Lee, K.H., Joeong, H.W., Lee, S.Y., Park, S.W., "Metabolite essentiality elucidates robustness of  Escherichia coli metabolism", Proc. Nat. Acad. Sci. (PNAS), 104 (34): 13638-13642 (2007. 8.), http://dx.doi.org/10.1073/pnas.0703262104

198. Song, H., Huh, Y.S., Lee, S.Y., Hong, W.H., Hong, Y.K. "Recovery of succinic acid produced by fermentation of a metabolically engineered Mannheimia succiniciproducens strain", J. Biotechnol. 132: 445-452 (2007. 7.), http://dx.doi.org/10.1016/j.jbiotec.2007.07.496

197. Yun, H., Lee, J. W., Jeong, J., Chung, J., Park, J. M., Myoung, H. N., and Lee, S. Y., "EcoProDB: the Escherichia coli protein database", Bioinformatics, 23(18): 2501-2503 (2007. 9.), http://dx.doi.org/10.1093/bioinformatics/btm351

196. Jang, Y.-S., Jung, Y.R., Lee, S.Y., Kim, J.M., Lee, J.W., Oh, D.-B., Kang, H.A., Kwon, O., Jang, S.H., Song, H., Lee, S.J., and Kang, K.Y. "Construction and characterization of shuttle vectors for succinic acid producing rumen bacteria", Appl. Environ. Microbiol. 73(17): 5411-5420 (2007. 9.), http://dx.doi.org/10.1128/AEM.01382-07

195. Song, H., Lee, J.W., Choi, S., You, J.K., Hong, W.H., and Lee, S.Y., "Effects of dissolved CO2 levels on the growth of Mannheimia succiniciproducens and succinic acid production", Biotechnol. Bioeng, 98(6): 1296-1304 (2007. 12.), http://dx.doi.org/10.1002/bit.21530

194. Park, J.H., Lee, K.H., Kim, T.Y., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation", Proc. Nat. Acad. Sci. (PNAS), 104 (19): 7797-7802 (2007. 4.), http://dx.doi.org/10.1073/pnas.0702609104

193. Kim, T.Y., Kim, H.U., Park, J.M., Song, H., Kim, J.S. and Lee, S.Y., "Genome-scale analysis of Mannheimia succiniciproducens metabolism", Biotechnol. Bioeng. (Accelerated Publication), 97(4): 657-671 (2007. 7.), http://dx.doi.org/10.1002/bit.21433

192. Choi, H.S., Kim, T.Y., Lee, D.-Y., and Lee, S.Y., "Incorporating metabolic flux ratios into constraint-based flux analysis by using artificial metabolites and converging ratio determinants", J. Biotechnol. 129(4):696-705 (2007. 5.), http://dx.doi.org/10.1016/j.jbiotec.2007.02.026

 191. Baek, J. H. and Lee, S. Y., "Transcriptome analysis of phosphates starvation response in Escherichia coli", J. Microbiol. Biotechnol. 17(2): 244-252 (2007. 2.)

190. Keum, K.C., Yoo, S.M., Lee, S.Y., Chang, K.H., Yoo, N.C., Yoo, W.M., Kim, J.M., Choi, J.Y., Kim, J.S. and Lee, G. "DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii", Mol. Cell. Probes, 20: 42-50 (2006. 2.), http://dx.doi.org/10.1016/j.mcp.2005.09.001

189. Lee, S.Y., and Jang, S.H. "White biotechnology", Asia Pacific Biotech News, 10: 559-563 (2006. 5.)

188. Jiang, H., Shang, L., Yoon, S.H., Lee, S.Y., Yu Z., "Optimal production of poly-gamma-glutamic acid by metabolically engineered Escherichia coli", Biotechnol Lett., 28(16):1241-1246. (2006. 8.), http://dx.doi.org/10.1007/s10529-006-9080-0

187. Lee, S.Y., "Deciphering bioplastic production", Nature Biotechnol. 24 (10): 1227-1229 (2006. 10.)

186. Park, T.J., Lee, S.Y., Lee, S.J., Park, J.P., Yang, K.S., Lee, K.-B., Ko, S., Park, J.B., Kim, T., Kim, S.K., Shin, Y.B., Chung, B.H., Ku, S.-J., Kim, D.H. and Choi, I.S., "Protein nanopatterns and biosensors using gold binding polypeptide as a fusion partner", Anal. Chem. 78: 7197-7205. (2006. 10.), http://dx.doi.org/10.1021/ac060976f

185. Baek, J.H. and Lee, S.Y., "Novel gene members in the Pho regulon of Escherichia coli", FEMS Microbiol. Lett. 264: 104-109 (2006. 11.), http://dx.doi.org/10.1111/j.1574-6968.2006.00440.x

184. Kwon, Y.D., Lee, S.Y. and Kim, P. "Influence of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) expression on succinic acid fermentation in Escherichia coli under high bicarbonate condition", J. Microbiol. Biotechnol. 16(9): 1448-1452 (2006. 9.)

183. Barrett, C.L., Kim, T.Y., Kim, H.U., Palsson, B.O., and Lee, S.Y., "Systems biology as a foundation for genome-scale synthetic biology", Curr. Opin. Biotechnol. 17: 488-492 (2006. 10.), http://dx.doi.org/10.1016/j.copbio.2006.08.001

182. Kim, J.-B., Ganesan, R., Yoo, S.Y., Choi, J.-H., and Lee, S.Y., "Simple patterning of cells on a biocompatible nonchemically amplified resist", Macromol. Rapid Comm. 27: 1442-1445 (2006. 9.), http://dx.doi.org/10.1002/marc.200600364

181. Park, T.J., Park, J.P., Seo, G.-M., Chai, Y.G., and Lee, S.Y., "Rapid and accurate detection of Bacillus anthracis spores using peptide-quantum dot conjugates", J. Microbiol. Biotechnol. 16(11): 1713-1719 (2006. 11.)

180. Huh, Y.S., Jun, Y.-S., Hong, Y.K. Song, H., Lee, S.Y., Hong, W.H., "Effective purification of succinic acid from fermentation broth produced by Mannheimia succiniciproducens", Process Biochemistry 41: 1461-1465(2006. 6.), http://dx.doi.org/10.1016/j.procbio.2006.01.020

179. Kim, T.Y. and Lee, S.Y., "Accurate metabolic flux analysis through data reconciliation of isotope balance-based data", J. Microbiol. Biotechnol. 16(7): 1139-1143 (2006. 7.)

178. Kim, J.S., Yun, H., Kim, H.U., Choi, H.S., Kim, T.Y., Woo, H.M., and Lee, S.Y., "Resources for systems biology research", J. Microbiol. Biotechnol. 16(6): 832-848 (2006. 6.)

177. Park, T.J., Park, J.P., Lee, S.J., Hong, H.J. and Lee, S.Y., "Polyhydroxyalkanoate chip for the specific immobilization of recombinant proteins and its applications in immunodiagnostics", Biotechnol. Bioprocess Eng., 11: 173-177 (2006. 6.)

176. Lee, D.-Y., Yun, C., Cho, A., Hou, B.K., Park, S. and Lee, S.Y., "WebCell: a web-based environment for kinetic modeling and dynamic simulation of cellular networks", Bioinformatics, 22(9): 1150-1151 (2006. 4. 1.), http://dx.doi.org/10.1093/bioinformatics/btl091

175. Han, M.-J. and Lee, S.Y., "The Escherichia coli Proteome: Past, Present, and Future Prospects", Microbiol. Mol. Biol. Reviews, 70(2): 362-439 (2006. 6.), http://dx.doi.org/10.1128/MMBR.00036-05

174. Lee, J.W., Lee, S.Y., Song, H., and Yoo, J.S. "The proteome of Mannheimia succiniciproducens, a capnophilic rumen bacterium", Proteomics, 6: 3550-3566  (2006. 6.), http://dx.doi.org/10.1002/pmic.200500837

173. Kim, J.S. and Lee, S.Y., "Genomic tree of gene contents based on the functional groups of KEGG orthology", J. Microbiol. Biotechnol., 16(5) 748-756 (2006. 5.)

172. Han, M.-J., Park, S.J., Lee, J.W., Min, B.H., Lee, S.Y., Kim, S.-J., and Yoo, J.-S. "Analysis of poly(3-hydroxybutyrate) granule-associated proteome in recombinant Escherichia coli", J. Microbiol. Biotechnol. 16(6): 901-910 (2006. 6.)

171. Lee, S.J., Song, H. and Lee, S.Y., "Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production", Appl. Environ. Microbiol. 72(3): 1939-1948 (2006. 3.), http://dx.doi.org/10.1128/AEM.72.3.1939-1948.2006

170. Wong Po Foo, C.,  Bini, E.,  Huang, J.,  Lee, S.Y. and Kaplan, D.L.,  "Solution behavior of synthetic silk peptides and modified recombinant silk proteins" Appl. Phys. A 82(2): 193-203 (2006. 2.), http://dx.doi.org/10.1007/s00339-005-3425-8

169. Choi, J.H., Keum, K.C., and Lee, S.Y., "Production of recombinant proteins by high cell density culture of Escherichia coli", Chem. Eng. Sci., 66: 876-885 (2006. 2.), http://dx.doi.org/10.1016/j.ces.2005.03.031

168. Song, H. and Lee, S.Y., "Production of succinic acid by bacterial fermentation", Enzyme Microb. Technol.,39: 352-361(2006. 7.)

167. Lee, D.-Y., Zimmer, R., Lee, S.Y. and Park, S., "Colored petri net modeling and simulation of signal transduction pathways", Metab. Eng., 8: 112-122 (2006. 3.)

166. Lee, S.Y., Woo, H.M., Lee, D.Y., Choi, H.S., Kim, T.Y., Yun, H.S., "Systems-level analysis of genome-scalein silico metabolic models using MetaFluxNet", Biotechnol. Bioproc. Eng., 10: 425-431 (2005.10)

165. Lee, S.Y., Woo, H.M., Lee, D.-Y., Choi, H.S., Kim, T.Y., and Yun, H., "Systems-level analysis of genome-scale microbial metabolisms under the integrated software environment", Biotechnol. Bioprocess Eng. 10(5): 425-431 (2005. 9~10)

164. Han, M.-J., Lee, J.W., and Lee, S.Y., "Enhanced Proteome Profiling by Inhibiting Proteolysis with Small Heat Shock Proteins", J. Proteome Res., 4(6): 2429-2434 (2005. 12.)

163. Lee, S.H., Lee, S.Y. and Park, B., "Cell surface display of lipase on the Pseudomonas putida using OprF as an anchoring motif and its biocatalytic applications", Appl. Environ. Microbiol., 71(12): 8581-8586 (2005. 12.)

162. Fan, L.T., Shafie, S., Bertok, B., Friedler, F., Lee, D.-Y., Seo, H., Park, S., and Lee, S.Y., "Graph-theoretic approach for identifying catalytic or metabolic pathways", J. Chin. Inst. Eng., 28(7): 1021-1037(2005. 12.)

161. Chen, S., Zhou, X.F., Jeong, H.Y., Choi, K.R., Lee, S.Y. and Deng, Z.X., "Sequence analysis of a region upstream of antibiotic FR0008 Gene cluster", Journal of Agricultural Biotechnology, 12(3): 404-405 (2005.)

160. Lee, S.J., Lee, D.-Y., Kim, T.Y., Kim, B.H., Lee, J., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the enhanced production of succinic acid based on genome comparison and in silico gene knock-out simulation", Appl. Environ. Microbiol., 71(12): 7880-7887 (2005. 12.)

159. Park, S.J., Lee, S.Y., Cho, J., Kim, T.Y., Lee, J.W., Park, J.H., and Han, M.-J., "Global physiological understanding and metabolic engineering of microorganisms based on omics studies", Appl. Microbiol. Biotechnol., 68:567-579 (2005. 9.)

158. Lee, S.J., Park, J.P., Park, T.J., Lee, S.Y., Lee, S., and Park, J.K., "Selective immobilization of fusion proteins on polyhydroxyalkanoate microbeads", Anal. Chem., 77: 5755-5759 (2005. 9. 1.)

157. Yun, H., Lee, D.-Y., Jeong, J., Lee, S., and Lee, S.Y., "MFAML: a standard data structure for representing and exchanging metabolic flux models", Bioinformatics, 21(15): 3329-3330 (2005. 8. 1.)

156. Park, J.P., Lee, K.-B., Lee, S.J., Park, T.J., Kim, M.G., Chung, B.H., Lee, Z.-W., Choi, I.S., and Lee, S.Y., "Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner", Biotechnol. Bioeng. 92(2): 160-165 (2005. 10. 20.)

155. Jin, J.H., Jung, U.S., Nam, J.W., In, Y.H., Lee, S.Y., Lee, D.H., and Lee, J.W., "Construction of comprehensive Metabolic Network for Glycolysis with Regulation Mechanisms and Effectors", J. Microbiol. Biotechnol., 15(1): 161-174(2005. 2.)

154. Lee, S.Y., Lee, D.-Y., and Kim, T.Y., "Systems biotechnology for strain improvement", Trends Biotechnol., 23(7): 349-358 (2005. 7.)

153. Lee, D.-Y., Fan, L.T., Park, S., Lee, S.Y., Shafie, S., Bertok, B., and Friedler, F., "Complementary identification of multiple flux distributions and multiple metabolic pathways", Metab. Eng., 7:182-200 (2005. 5.)

152. Park, S.J. and Lee, S.Y., "Systems biological approach for the production of various polyhydroxyalkanoates by metabolically engineered Escherichia coli", Macromol. Sympo., 224:1-9 (2005. 4.)

151. Lee, S.H., Choi, J.-I., Han, M.-J., Choi, J.H., and Lee, S.Y., "Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its applications to enantioselective resolution in organic solvent", Biotechnol. Bioeng., 90(2): 223-230(2005. 8. 20.)

150. Choi, J.H., Choi, J.-I., and Lee, S.Y., "Display of proteins on the surface of Escherichia coli by C-terminal deletion fusion to the Salmonella typhimurium OmpC", J. Microbiol. Biotechnol., 15(1):141-146 (2005. 2.)

149. Park, S.J., Choi, J.-I., and Lee, S.Y., "Short-chain length polyhydroxyalkanoates: synthesis in metabolically engineered Escherichia coli and medical applications", J. Microbiol. Biotechnol., 15(1):206-215 (2005. 2)

148. Jin, J.H., Choi, K.K., Jung, U.S., In, Y.H., Lee, S.Y. and Lee, J., "Regulatory analysis of amino acid synthesis pathway in Escherichia coli: aspartate family", Enzyme Microb. Technol. 35: 694-706 (2004. 12. .1)

147. Hong, S. H.,  Kim, J.S.,  Lee, S.Y., In, Y.H., Choi, S.S., Rih, J.K., Kim, C. H.,  Jeong, H., Hur, C. G., and Kim, J.J., "The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens", Nature Biotechnol. 22(10): 1275-1281 (2004. 10.)

146. Park, S.J., Choi, J.-I., and Lee, S.Y., "Engineering of Escherichia coli fatty acid metabolism for the production of polyhydroxyalkanoates", Enzyme Microb. Technol., 36(4): 579-588 (2004. 3. 2.)

145. Jung, D.-H., Kim, B. H., Ko, Y. K.,  Jung, M. S., Jung, S. O.,  Lee, S. Y., and Jung, H.-T., "Covalent attachment and hybridization of DNA oligonucleotides on patterned single-walled carbon nanotube films", Langmuir, 20(20): 8886-8891 (2004. 9. 28.)

144. Park, T.J., Lee, K.-B., Lee, S.J., Park, J.P., Lee, Z.-W., Lee, S.Y. and Choi, I.S., "Micropatterns of spores displaying heterologous proteins", J. Am. Chem. Soc., 126(34):10512-10513 (2004. 9. 1.)

143. Kim, D.Y., Yim, S.C., Lee, P.C., Lee, W.G., Lee, S.Y. and Chang, H.N., "Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens MBEL55E", Enzyme Microb. Technol. 35: 648-653 (2004. 12. 1.)

142.  Nam, J.W., Han, K.H., Yoon, E.S., Shin, D.I., Jin, J.H., Lee, D.H., Lee, S.Y. and Lee, J. "In silico analyis of lactate producing metabolic network in Lactococcus lactis", Enzyme Microb. Technol. 35: 654-662 (2004. 12. 1.)

141. Hong, S.H. and Lee, S.Y., "Enhanced production of succinic acid by metabolically engineered Escherichia coli with amplified activities of malic enzyme and fumarase", Biotechnol. Bioprocess Eng., 9:252-255 (2004. 7~.8)

140. Hou, B.K., Kim, J.S., Jun, J.H., Lee, D.-Y., Kim, Y.W., Chae, S., Roh, M., In, Y.-H., and Lee, S.Y., "BioSilico: an integrated metabolic database system", Bioinformatics, 20(17) : 3270-3272 (2004. 11. 22.)

139. Choi, J.-I. and Lee, S.Y., "High level production of supra molecular weight poly(3-hydroxybutyrate) by metabolically engineered Escherichia coli", Biotechnol. Bioprocess Eng., 9:196-200 (2004.3)

138. Lee, S.H., Choi, J.H., Park, S.H., Choi, J.-I., and Lee, S.Y., "Enantioselective resolution of racemic compounds by cell surface displayed lipase", Enzyme Microb. Technol., 35 : 429-436 (2004. 10. 6.)

137. Han, M.-J., Park, S.J., Park, T.J., and Lee, S.Y., "Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli", Biotechnol. Bioeng. 88(4): 426-436 (2004.11.20.)

136. Lee, S.H., Choi, J.-I., Park, S.J., Lee, S.Y. and Park, B.C. "Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and use of the enzyme in enantioselective biocatalysis", Appl. Environ. Microbiol., 70(9) : 5074-5080 (2004. 9.)

135. Hong, S.H., Kim, T.Y., and Lee, S.Y., "Phylogenetic analysis based on genome-scale metabolic pathway reaction content", Appl. Microbiol. Biotechnol., 65(2): 203-210 (2004.8)

134. Park, J.P., Lee, S.J., Park, T.J., Lee, K.B., Choi, I.S., Lee, S.Y., Kim, M.G., and Chung, B.H., "Microcontact Printing of Biotin for Selective Immobilization of  Streptavidin-fused Proteins and SPR Analysis",  Biotechnol. Bioprocess Eng., 9:137-142 (2004. 3.~4.)

133. Yoo, S.M., Keum, K.C., Yoo, S.Y., Choi, J.Y., Chang, K.H., Yoo, N.C., Yoo, W.M., Kim, J.M., Lee Duke, and Lee, S.Y., "Development of DNA Microarray for Pathogen Detection", Biotechnol. Bioprocess Eng., 9:93-99 (2004. 3.~4.)

132. Kim, B. S., Lee, S. C., Lee, S. Y., Chang, Y. K., and Chang, H. N., "High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat", Bioprocess Biosyst. Eng., 26:147-150 (2004. 4.)

131. Lee, S.J. and Lee, S.Y., "Microarrays of peptides elevated on the protein layer for efficient protein kinase assay", Anal. Biochem., 330:311-316 (2004. 7. 15.)

130. Jeong, K.J., Choi, J.H., Yoo, W.M., Keum, K.C., Yoo, N.C., Lee, S.Y. and Sung, M.-H., "Constitutive Production of Human Leptin by Fed-batch Culture of Recombinant rpoS- Escherichia coli", Protein Expression Purif. 36 : 150-156 (2004.7)

129. Park, S.J. and Lee, S.Y., "New FadB homologous enzymes and their use in enhanced biosynthesis of medium-chain-length polyhydroxyalkanoates in fadB mutant Escherichia coli", Biotechnol. Bioeng., 86:681-686 (2004. 6. 20.)

128. Lee, S.Y., Hong, S.H., Lee, S.H., and Park, S.J., "Fermentative Production of Chemicals That Can Be Used for Polymer Synthesis", Macromol. Biosci., 4 : 157-164 (2004. 3. 15.)

127. Lee, S.J. and Lee, S.Y., "Micro total analysis system (μ-TAS) in biotechnology", Appl. Microbiol. Biotechnol., 64 : 289-299 (2004. 4.)

126. Choi, J.H. and Lee, S.Y., "Secretory and extracellular production of recombinant proteins using Escherichia coli", Appl. Microbiol. Biotechnol., 64 : 625-635 (2004. 6.)

125. Park, S.J., Lee, S.Y. and Lee, Y., "Biosynthesis of (R)-3-Hydroxyalkanoic Acids by Metabolically Engineered Escherichia coli", Appl. Biochem. Biotechnol., 114(1-3) : 373-379 (2004. 3.)

124. Park, S.J. and Lee, S.Y., "Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by metabolically engineered Escherichia coli strains", Appl. Biochem. Biotechnol., 114(1-3) : 335-346 (2004. 3.)

123. Lee, D.Y, Sung, S,W., Lee, S.Y. and Park, S., "Combined Deterministic-Stochastic Approach for Pharmacokinetic Modeling", Ind. Eng. Chem. Res., 43 : 1133-1143 (2004. 2. 18.)

122. Chen, S., Huang, X., Zhou, X., Bai, L., Jing He, Jeong, K. J., Lee, S.Y., and Deng, Z., "Organizational and Mutational Analysis of a Complete FR-008/Candicidin Gene Cluster Encoding a Structurally Related Polyene Complex", Chemistry and Biology, 10 : 1065-1076 (2003. 11.)

121. Lee, P.C., Lee, S.Y., Hong S.H., and Chang, H.N., "Batch and continuous cultures of Mannheimia succiniciproducens MBEL 55E for the production of succinic acid from Whey and corn steep liquor", Bioprocess Biosyst. Eng., 26 : 63-67 (2003. 11.)

120. Han, M.J., Jeong, K.J., Yoo, J.S., and Lee. S.Y., "Engineering Escherichia coli for increased production of serine-rich proteins based on proteome profiling", Appl. Environ. Microbiol., 69(10) : 5772-5781 (2003. 10.)

119. Park, S.J., and Lee, S.Y., "Identification and characterization of a new Enoyl-Coenzyme A hydratase involved in the biosynthesis of medium-chain-length polyhydroxyalkanoates in recombinant Escherichia coli", J. Bacteriol., 185(18) : 5391-5397 (2003. 9.)

118. Choi, J.H., Lee, S.J., Lee, S.J., and Lee, S.Y., "Enhanced production of insulin-like growth factor I fusion protein in Escherichia coli by the coexpression of the down regulated genes identified by transcriptome profiling", Appl. Environ. Microbiol., 69(8) : 4737-4742 (2003. 8.)

117. Han, M.J. and Lee, S.Y., "Proteome profiling and its use in metabolic and cellular engineering", Proteomics, 3 : 2317-2324 (2003. 12.)

116. Park, S.J., Lee, S.Y. and Chang, Y.K., "Desulfurization of dibenzothiophene and diesel oil by metabolically engineered Escherichia coli", J. Microbiol. Biotechnol., 13(4) : 578-583 (2003. 8.)

115. Hong, S.H., Moon, S.Y., and Lee, S.Y., "Prediction of maximum yields of metabolites and optimal pathways for their production by metabolic flux analysis", J. Microbiol. Biotechnol., 13(4) : 571-577 (2003. 8.)

114. Lee, D.Y, Yun, H. S, Lee, S.Y. and Park, S.W., "MetaFluxNet: the management of metabolic reaction information and quantitative metabolic fluxanalysis", Bioinformatics, 19(16) : 2144-2146 (2003. 11. 1.)

113. Park, S.J., Park, J.P., Lee, S.Y. and Doi, Y., "Enrichment of specific monomer in medium-chain-length poly (3-hydroxyalkanoates) by amplification of fadD and fadE genes in recombinant Escherichia coli", Enzyme Microb. Technol., 33 : 62-70 (2003. 7. 16.)

112. Hong, S.H., Park, S.J., Moon, S.Y., Park, J.P., and Lee, S.Y., "In silico prediction and validation of the importance of Entner-Doudoroff pathway in poly(3-hydroxybutyrate) production by metabolically engineered Escherichia coli", Biotech. Bioeng., 83(7) : 854-863 (2003. 9. 30.)

111. Lee, S.Y. and Lee, Y., "Metabolic engineering of Escherichia coli for production of enantiomerically pure (R)-(-)-hydroxycarboxylic acids", Appl. Environ. Microbiol., 69(6) : 3421-3426 (2003. 6.)

110. Jeong, K.J. and Lee, S.Y., "Enhanced production of recombinant proteins in Escherichia coli by filamentation suppression", Appl. Environ. Microbiol. 69(2) : 1295-1298 (2003. 2.)

109. Lee, P.C., Lee, S.Y., Hong, S.H., and Chang, H.N., "Biological conversion of wood hydrolysate to succinic acid by Anaerobiospirillum succiniciproducens", Biotechnol. Lett., 25 : 111-114 (2003. 1.)

108. Lee, S.Y., Choi, J.H., and Xu, Z., "Microbial cell-surface display", Trends Biotechnol., 12(1) : 45-52 (2003. 1.)

107. Yoon,S.H., Han, M.J., Lee, S.Y., Jeong, K.J., and Yoo, J.S., "Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture", Biotechnol. Bioeng., 81(7) : 753-767 (2003. 3. 30.)

106. Kim, J. S., Yoon S. H., and Lee, S. Y., "Development of database systems by integrating heterogeneous metabolic databases", Genome Informatics,13: 486-487 (2002. 12.)

105. Heo, W.J., Yoon, S.H., and Lee, S.Y., "Global gene expression profiling of E. coli and its mutant unable to produce acetate", Genome Informatics,13: 270-271 (2002. 12.)

104. Lee, D.Y., Oh, Y.G., Yoon, H.S., Lee, S.Y. and Park, S.W., "Exploring Flux Distribution Profiles for Switching Pathways Using Multiobjective Flux Balance Analysis", Genome Informatics,13: 363-364 (2002. 12.)

103. Yoon, S.H. and Lee S.Y., "Comparison of transcript levels by DNA microarray and metabolic flux based on flux analysis for the production of poly-γ-glutamic acid in recombinant Escherichia coli", Genome Informatics,13: 587-588 (2002. 12.)

102. Yoo, S.Y., Chang, K. H., Yoo, S.M. and Park, S.Y., Yoo, N.C., Keum, K.C., Yoo W.M., Kim, J. M., and Lee,S.Y., "Design of ITS and 23S rDNA-targeted probes and its usefulness for the identification of bacterial pathogens", Genome Informatics,13:589-590 (2002. 12.)

101. Lee, S.Y., Hong S.H., and Moon, S.Y., "In Silico Metabolic Pathway Analysis and Design: Succinic acid Production by Metabolically Engineered Escherichia coli as an example", Genome Informatics, 13: 214-223 (2002. 12.)

100. Jung, H., Lee, S.Y., Kaler, E.W., Coldren, B., and Zasadzinski, J. A., "Gaussian curvature and the equilibrium among bilayer cylinders, spheres, and discs", Proc. Nat. Acad. Sci., 99 (24): 15318-15322 (2002. 11. 26.) 

99. Jeong, K.J. and Lee, S.Y., "Excretion of Human β-Endorphin into Culture Medium by Using Outer Membrane Protein F as a Fusion Partner in Recombinant Escherichia coli ", Appl. Environ. Microbiol., 68(10):4979-4985 (2002. 10) 

98. Park, S.J., Park, J.P., and Lee, S.Y., "Metabolic engineering of  Escherichia coli  for the production of medium -chain-length polyhydroxyalkanoates rich in specific monomers", FEMS Microbiol. Lett., 214:217-222 (2002. 9. 10) 

97. Lee, P.C., Lee, S.Y., Hong, S.H., and Chang, H.N., "Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducens MBEL55E, from bovine rumen", Appl. Microbiol. Biotechnol., 58:663-668 (2002. 4.) 

96. Hong, S.H. and Lee, S.Y., "Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli", Appl. Microbiol. Biotechnol., 58:286-290 (2002. 3.) 

95.Park, S.J., Park, J.P., and Lee, S.Y., "Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermentor", Biotechnol, Lett., 24:185-189 (2002. 2.) 

94. Kim, J. S. and Lee, S.Y., "In silico metabolic pathway modeling and analysis of Mycoplasma pneumoniae", Genome Informatics,12: 298-299 (2001. 12.) 

93. Do, J.H., Chang, H.N., and Lee, S.Y., "Efficient recovery of g-poly(glutamic acid) from highly viscous culture broth", Biotechnol. Bioeng., 76:219-223 (2001. 11.) 

92. Chen, G.Q., Zhang, G., Park, S.J., and Lee, S.Y., "Industrial scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)", Appl. Microbiol. Biotechnol., 57:50-55 (2001. 10.) 

91.    Yim, S.C., Jeong, K. J., Chang, H.N., and Lee. S.Y., "High level secretory production of human G-CSF by fed-batch culture of recombinant Escherichia coli", Bioprocess Biosyst. Eng., 24(4):249-254 (2001. 11.) 

90. Jeong, K.J. and Lee, S.Y., "Secretory production of human granulocyte-colony stimulating factor in Escherichia coli", Protein Expres. Purif., 23(2):311-318, November (2001. 11.) 

89.  Park, S.H., Lee, S.H., and Lee, S.Y., "Preparation of optically active beta-amino acids from microbial polyester polyhydroxyalkanoates", J. Chem. Research-S, 11:498-499 (2001. 11.) 

88. Park, S. H., Lee, S. Y. and Bose, A.K., "An efficient and eco-friendly approach to 15N-unsubstituted β-lactams : 15N- labeled synthons for taxol and its analogs", Bull. Korean Chem. Soc., 22(5):493-498 (2001. 5. 20.) 

87. Van Wegen, R. J., Lee, S. Y. and Middelberg, A., "Metabolic and kinetic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli", Biotechnol. Bioeng.,  74(1):70-80 (2001. 7. 5.) 

86.  Lee, S.Y., Choi, J.,  "Production of Microbial polyester by fermentation of recombinant microorganism", Adv. Biochem. Eng. Biotechnol., 71:183-207 (2001) 

85.  Lee, P.C., Lee, W.G., Lee, S.Y. and  Chang, H.N., "Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source", Biotechnol. Bioeng. 72(1):41-48 (2001. 6.) 

84.  Ahn, W.S., Park, S.J., and Lee, S.Y., "Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli", Biotechnol. Lett., 23:235-240 (2001. 2.) 

83.  Park, S.J., Ahn, W.S., Green, P., and Lee, S.Y., "Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains", Biotechnol. Bioeng., 74(1):81-86 (2001. 7. 5.) 

82.  Han, M.J., Yoon, S.S., and Lee, S.Y., "Proteome analysis of metabolically engineered Escherichia coli  producing poly(3-hydroxybutyrate)", J. Bacteriol., 183(1):301-308 (2001. 1.) 

81.  Hong, S.H. and Lee, S.Y., "Metabolic flux analysis for succinic acid production by recombinant Escherichia coli with amplified malic enzyme activity", Biotechnol. Bioeng., 74(2):89-95(2001. 7. 20) 

80.  Park, S. J., Ahn, W. S., Green, P. R., and Lee, S. Y., "Production of poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains" Biomacromolecules, 2:248-254 (2001. SPR)  

79.  Jeong, K.J. and Lee, S.Y.,"Secretory production of human leptin in Bacillus subtilis", J. Microbiol. Biotechnol., 10(6):753-758 (2000. 12.) 

78.  Hong, S.H. and Lee, S.Y.,  "Metabolic Flux distribution in metabolically engineered Escherichia coli strain producing succinic acid", J. Microbiol. Biotechnol., 10(4):496-501 (2000. 8.) 

77.  Lee, P.C., Lee, W.G., Kwon, S.H., Lee, S.Y. and Chang, H.N., "Batch and Continuous cultivation of Anaerobiospirillum succiniproducens for the production of succinic acid from whey", Appl. Microbiol. Biotechnol., 54(1):23-27 (2000. 7.) 

76.  Lee, S.Y., Wong, H.H., Choi, J., Lee, S.H., Lee, S.C., and Han, C.S., "Production of medium-chain-length  polyhydroxyalkanoates by high cell density cultivation of  Peudomonas putida under phosphorus limitation", Biotechnol. Bioeng., 68:466-470 (2000. 5. 20.) 

75.   Xu, Z., Lee, S.Y. and Yu, Z., "Physiological characteristics of recombinant Escherichia coli cells displaying poly-His peptides", Biotechnol. Lett., 21:1091-1094 (1999. 12.) 

74.  Choi, J., and Lee, S.Y.,  "Economic considerations in the production of poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by bacterial fermentation", Appl. Microbiol. Biotechnol., 53: 646-649 (2000.6.) 

73.   Yoon, S. H., Do, J. H., Lee, S. Y. and Chang, H. N., "Production of poly-γ-glutamic acid by fed-batch culture of Bacillus licheniformis", Biotechnol. Lett., 22(7):585-588 (2000. 4.) 

72.  Lee, S.Y., Choi, J., and Lee, S.H., "Production of polyhydroxyalkanoates by fermentation of bacteria", Macromol. Symp., 159:259-266 (2000. 10.) 

71.   Lee, Y., Park, S.H., Lim, I.T., Han, K., and Lee, S.Y.,  "Preparation of alkyl (R)-(-)-3-hydroxybutyrate by  acidic alchoholysis of poly-(R)- (-)-3-hydroxybutyrate", Enzyme Microb. Technol., 27:33-36 (2000. 7.) 

70.  Chung, B.H., Choi, Y.J., Yoon, S.H., Lee, S.Y. and Lee, Y.I., "Process development for production of recombinant human insulin-like growth factor-I in Escherichia coli", J. Ind. Microbiol. Biotechnol., 24:94-99 (2000. 2.) 

69.  Kim, B.S., O'Neill, B.K., and Lee. S.Y., "Increasing poly(3-hydroxybutyrate) accumulation in recombinant Escherichia coli from whey by agitation speed control", J. Microbiol. Biotechnol., 10(5):628-631 (2000. 10.) 

68.  Choi, J.H., Jeong, K.J., Kim, S.C., and Lee, S.Y.,  "Efficient secretory production of alkaline phosphatase by high cell density culture of recombinant Escherichia coli using the Bacillus sp. Endoxylanase signal sequence", Appl. Microbiol. Biotechnol. 53:640-645 (2000. 6.) 

67.  Lee, S.H., Oh, D.H., Ahn, W.S., Lee, Y., Choi, J., and Lee, S.Y., "Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high cell density cultivation of Aeromonas hydrophila", Biotechnol. Bioeng., 67:240-244(2000. 1. 20.) 

66.  Jeong, K.J. and Lee, S.Y.,  "Secretory Production of human leptin in Escherichia coli", Biotechnol. Bioeng., 67:398-407(2000.2.20) 

65.  Ahn, W.S., Park, S.J., and Lee, S.Y.,  "Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution", Appl. Environ. Microbiol., 66(8):3624-3627 (2000. 8.) 

64.  Yoon, S.H., Choi, J., and Lee, S.Y.,  "Development of DNA microarrayer", J. Microbiol. Biotechnol., 10(1):21-26 (2000. 2.) 

63.  Choi, J. and Lee, S.Y., "High level production of poly(3-hydroxybutyratre-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli", Appl. Environ. Microbiol. 65:4363-4368 (1999. 10.) 

62.  Wong, H.H., van Wegen, R.J., Choi, J., Lee, S.Y. and Middelberg, A.P.J., "Metabolic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli",  J. Microbiol. Biotechnol., 9(5):593-603 (1999. 10) 

61.   Choi, J. and  Lee, S.Y., "Production of poly(3-hydroxybutyrate) with high P(3HB) content by recombinant Escherichia coli harboring the Alcaligenes latus P(3HB) biosynthesis genes and the Escherichia coli ftsZ gene", J. Microbiol. Biotechnol., 9(6):722-725 (1999. 12.) 

60.  Lee, S.Y., Lee, Y., and Wang, F., "Chiral compounds from bacterial polyesters: Sugars to plastics to fine chemicals", Biotechnol. Bioeng., 65:363-368 (1999. 11. 5.) 

59.  Xu, Z. and Lee, S.Y.,  "Display of polyhistidine peptides on Escherichia coli cell surface by using outer membrane protein C (OmpC) as an anchoring motif", Appl. Environ. Microbiol., 65:5142-5147 (1999. 11.) 

58.  Jeong, K.J. and Lee, S.Y.,  "High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification:", Appl. Environ. Microbiol., 65(7) : 3027-3032 (1999. 7.) 

57.  Lee, P.C., Lee, W.G., Lee, S.Y. and Chang, H.N., "Effects of medium components on the growth of Anaerobiospirillum succiniciproducens and succinic acid production", Process Biochem., 35:49-55 (1999. 10.) 

56.  Lee, S.Y., Choi, J., Han, K., and Song, J.Y., "Removal of endotoxin during purification of poly(3-hydroxybutyrate) from gram-negative bacteria", Appl. Environ. Microbiol., 65(6):2762-2764 (1999. 6.) 

55.  Lee, Y., Lee, S.Y. and Yang, J., "Production of rhamnolipid biosurfactant by fed-batch culture of Pseudomonas aeruginosa using glucose as a sole carbon source", Biosci. Biotechnol. Biochem. 63(5): 946-947(1999. 5.) 

54.  Park. S.J., Georgiou, G., and Lee, S.Y.,  "Secretory production of recombinant protein by high cell density culture of a protease negative mutant Escherichia coli strain", Biotechnol. Prog., 15(2): 164-167 (1999. 3.~4.) 

53.  Lee, S.Y.  and Choi, J., "Production and degradation of polyhydroxyalkanoate in waste environment", Waste Management, 19: 133-139 (1999) 

52.  Lee, P.C., Lee, W.G., Kwon, S., Lee, S.Y. and Chang, H.N., "Succinic acid production by Anaerobiospirillum succiniciproducens : Effects of the H2/CO2 supplying and glucose concentration", Enzyme Microb. Technol. 24: 549-554 (1999. 6.) 

51.   Lee, S.Y.  Choi, J., and Wong, H.H., "Recent advances in polyhydroxyalkanoate production by bacterial fermentation: mini-review" Int. J. Biol. Macromol. 25:31-36 (1999. 7.) 

50.   Lee, J., Lee, S.Y., Park, S., and Middelberg, A.P.J., "Control of fed-batch fermentations", Biotechnol. Adv. 17(1):29-48 (1999. 4.) 

49.  Choi, J. and Lee, S.Y., "Factors affecting the economics of polyhydroxyalkanoates production by bacterial fermentation" Appl. Microbiol. Biotechnol. 51: 13-21 (1999. 1.) 

48.  Choi, J. and Lee, S.Y.,  "Efficient and economical recovery of Poly(3-hydroxybutylate) from recombinant Escherichia coli by simple digestion with chemicals", Biotechnol. Bioeng. 62: 546-553 (1999. 3.) 

47.  Park, S.J. and Lee, S.Y.,  "Efficient recovery of secretory recombinant protein from protease negative mutant Escherichia coli strains", Biotechnol. Techniques, 12: 815-818 (1998. 11.) 

46.  Choi, J., Lee, S.Y. and Han, K., "Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for the enhanced production of Poly(3-hydroxybutyrate) in Escherichia coli", Appl. Environ. Microbiol., 64(12): 4897-4903 (1998. 12.) 

45.  Jeong, K.J., Lee, H.S., Lee, S.Y. and Chang, Y.K., "Development of recombinant bacteria for the degradation of dibenzothiophene", Ann. NY Acad. Sci., 864: 375-378 (1998)

44.  Kim, Y.C., Kwon, S., Lee, S.Y. and Chang, H.N., "Improved production of a bioadhesive precursor protein by fed-batch cultivation of recombinant Escherichia coli with a pLysS vector", Biotechnol. Lett., 20(8): 799-803 (1998. 8.) 

 43.  Lee, S.Y., Chang, H.N., Um, Y.S., and Hong, S.H., "Bacteriorhodopsin production by cell recycle culture of Halobacterium halobium", Biotechnol. Lett., 20(8): 763-765 (1998. 8.) 

42.  Wong, H.H. and Lee, S.Y., "Poly(3-hydroxybutyrate) production from whey by high density cultivation of recombinant Escherichia coli", Appl. Microbiol. Biotechnol., 50: 30-33 (1998. 7.) 

41.   Wong, H.H., Kim, Y.C., Lee, S.Y. and Chang, H.N., "Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli", Biotechnol. Bioeng. 60: 271-276 (1998. 11. 5.) 

40.  Lee, S. Y., "Poly(3-hydroxybutylate) production from xylose by recombinant Escherichia coli", Bioprocess Eng. 18: 397-399 (1998. 5.) 

39.  Lee, S. Y. and Choi, J., "Effect of fermentation of performance on the economics of poly(3-hydroxybutylate) production by Alcaligenes latus", Polym. Degrad. Stab. 59: 387-393 (1998. 1.) 

38.  Wang, F. and Lee, S.Y.,  "High cell density culture of metabolically engineered Escherichia coli for the production of poly(3-hydroxybutyrate) in a defined medium", Biotechnol. Bioeng., 58(2): 325-328 (1998. 4. 20.) 

37.  Lee, S. Y., Middelberg, A. P. J., and Lee, Y. K., "Poly(3-hydroxybutylate) production from whey using recombinant Escherichia coli", Biotechnol. Lett., 19(10): 1033-1035 (1997. 10.) 

36.  Wang, F. and Lee, S. Y., "Production of Poly(3-Hydroxybutyrate) by Fed-batch Culture of Filamentation-Suppressed Recombinant Escherichia coli" Appl. Environ. Microbiol., 63(12): 4765-4769 (1997. 12.) 

35.  Wang, F. and  Lee, S. Y., "Poly(3-hydroxybutyrate) production with high productivity and high polymer content by a fed-batch culture of Alcaligenes latus under nitrogen limitation" Appl. Environ. Microbiol., 63(9): 3703-3706 (1997. 9.) 

34.  Kusaka, S., Abe, H., Lee, S.Y. and Doi, Y., "Molecular mass of poly[(R)-3-hydroxybutyric acid] produced in a recombinant Escherichia coli", Appl. Microbiol. Biotechnol., 47: 140-143 (1997. 2.) 

33.  Choi, J. and Lee, S.Y.,  "Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation", Bioprocess Eng., 17(6): 335-342 (1997. 11.) 

32.  Lee, S.Y.,  "E. coli moves into the plastic age", Nature Biotechnol., 15: 17-18 (1997. 1.) 

31.   Lee, J., Lee, S.Y. and Park, S., "Fed-batch culture of Escherichia coli W by exponential feeding of sucrose as a carbon source", Biotechnol. Techniques, 11(1): 59-62 (1997. 1.) 

30.  Lee, S.Y., "Plastic bacteria?: Progress and prospects for polyhydroxyalkanoate production in bacteria", Trends Biotechnol., 14(11): 431-438 (1996. 11.) 

29.  Lee, I.Y., Kim, M.K., Park, Y.H., and Lee, S.Y.,  "Regulatory effects of cellular nicotinamide nucleotides and enzyme activities on poly(3-hydroxybutyrate) synthesis in recombinant Escherichia coli", Biotechnol. Bioeng., 52(6): 707-712 (1996. 12. 20.) 

28.  Lee, Y. and Lee, S.Y.,  "Enhanced production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinant Escherichia coli in a defined medium", J. Environ. Polym. Degrad. 4: 131-134 (1996. 4.) 

27.  Lee, S.Y., "High cell-density culture of Escherichia coli", Trends Biotechnol. 14(3): 98-105 (1996. 3.) 

26.  Lee, S.Y., "Poly(3-hydroxybutyrate) extrusion by cells of recombinant Escherichia coli", J. Microbiol. Biotechnol. 6(2): 147-149 (1996. 4.)

25.  Yim, K.S., Lee, S.Y. and Chang, H.N., "Synthesis of poly(3-hydroxybutyrate -co-3-hydroxyvalerate) by recombinant Escherichia coli", Biotechnol. Bioeng., 49(5): 495-503 (1996. 3. 5.) 

 24.  Lee, S.Y.,  "Bacterial polyhydroxyalkanoates", Biotechnol. Bioeng., 49(1): 1-14 (1996. 1. 5.) 

23.  Lee, S.Y.  and Chang, H.N., "Characteristics of poly(3-hydroxybutyric acid) synthesis by recombinant Escherichia coli", Ann. NY Acad. Sci., 782: 133-142 (1996) 

22.  Lee, S.Y.  and Chang, H.N., "Production of poly(3-hydroxybutyric acid) by recombinant Escherichia coli strains: genetic and fermentation studies", Can. J. Microbiol., 41(Suppl.1): 207-215 (1995. 1.) 

21.  Lee, S.Y.  and Chang, H.N., "Production of Poly-(hydroxyalkanoic acid)", Adv. Biochem. Eng. Biotechnol., 52: 27-58 (1995. 1.) 

20.  Middelberg, A.P.J., Lee, S.Y., Martin, J., Williams, D.R.G., and Chang, H.N., "Size analysis of poly(3-hydroxybutyric acid) granules produced in Escherichia coli", Biotechnol. Lett., 17(2): 205-210 (1995.2.) 

19.  Lee, S.Y., Lee, Y.K., and Chang, H.N., "Stimulatory effects of amino aciAppl. Environ.ds and oleic acid on poly(3-hydroxybutyric acid) synthesis by recombinant Escherichia coli", J. Ferment. Bioeng., 79(2): 177-180 (1995. 2.) 

18.  Hahn, S.K., Chang, Y.K., and Lee, S.Y., "Recovery and characterization of poly(3-hydroxybutyric acid) synthesized in Alcaligenes eutrophus and recombinant Escherichia coli", Appl. Environ. Microbiol., 61(1): 34-39 (1995. 1.) 

17.  Lee, S.Y., "Suppression of filamentation in recombinant Escherichia coli by amplified FtsZ activity", Biotechnol. Lett., 16(12): 1247-1252 (1994.12.) 

16.  Lee, S.Y.  and Chang, H.N., "Effect of complex nitrogen source on the synthesis and accumulation of poly(3-hydroxybutyric acid) by recombinant Escherichia coli in flask and fed-batch cultures", J. Environ. Polymer Degrad., 2(3): 169-176 (1994. 7.) 

15.  Lee, S.Y., Lee, K.M., Chang, H.N., and Steinbuchel, A., "Comparison of Recombinant Escherichia coli strains for synthesis and accumulation of poly-(3-hydroxybutyric acid), and morphological changes", Biotechnol. Bioeng., 44(11): 1337-1347 (1994. 12.) 

14.  Han, D.H., Lee, S.Y. and Hong, W.H., "Separation of intracellular proteins from Candida utilis using reverse micelles in a spray column", Biotechnol. Tech.,8(2): 105-110 (1994. 2.) 

13.  Kim, B.S., Lee, S.C., Lee, S.Y.,  Chang, H.N., Chang, Y.K., and Woo, S.I., "Production of poly-(3-hydroxybutyric-co-hydroxyvaleric acid) by fed-batch culture of Alcaligenes eutrophus with substrate feeding using on-line glucose analyzer", Enzyme Microb. Technol., 16(7): 556-561 (1994. 7.) 

12.  Kim, B.S., Lee, S.C., Lee, S.Y., Chang, H.N., Chang, Y.K, and Woo, S.I., "Production of poly(3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control", Biotechnol. Bioeng., 43: 892-898 (1994. 4. 15.) 

11.   Lee, S.Y.  and Chang, H.N., "Generation of bacteriophage λ lysogens by electroporation", BioTechniques, 16(2): 206-208 (1994. 2.) 

10.   Lee, S.Y., Chang, H.N., and Chang, Y.K., "Production of poly(β-hydroxybutyric acid) by recombinant Escherichia coli", Ann. NY Acad. Sci. 721: 43-53 (1994. 1) 

9.  Lee, S.Y., Yim, K.S., Chang, H.N., and Chang, Y.K., "Construction of plasmids, estimation of plasmid stability, and use of stable plasmids for the production of poly(3-hydroxybutyric acid) in Escherichia coli", J. Biotechnol., 32(2): 203-211 (1994. 2. 14) 

8.  Kang, B.C., Lee, S.Y. and Chang, H.N., "Production of Bacillus thuringiensis spores in total cell retention culture and two stage continuous culture using an internal ceramic filter system", Biotechnol. Bioeng., 42(9): 1107-1112 (1993. 11.) 

7.  Lee, S.Y.  and Chang, H.N., "High cell density cultivation of Escherichia coli W using sucrose as a carbon source", Biotechnol. Lett., 15(9): 971-974 (1993. 9.) 

6.  Han, D.H., Lee, S.Y. and Hong, W.H., "Direct recovery of intracellular proteins from Candida utilis using reverse micelles in combination with reducing agent", Biotechnol. Tech., 7(8): 545-550 (1993. 8.) 

5.  Lee, S.Y., Mermelstein, L.D., and Papoutsakis, E.T., "Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824", FEMS Microbiol. Lett., 108(3): 319-324 (1993. 4. 15.) 

4.  Kim, B.S., Lee, S.Y. and Chang, H.N., "Production of poly-β-hydroxybutyrate by fed-batch culture of recombinant Escherichia coli", Biotechnol. Lett., 14(9): 811-816 (1992. 9.) 

3.  Kang, B.C., Lee, S.Y. and Chang, H.N., "Enhanced spore production of Bacillus thuringiensis by fed-batch culture", Biotechnol. Lett., 14(8): 721-726  (1992. 8.) 

2.  Lee, S.Y., Bennett, G.N., and Papoutsakis, E.T., "Construction of E.coli-C.acetobutylicum shuttle vectors and transformation of C.acetobutylicum strains", Biotechnol. Lett. 14(5): 427-432 (1992. 5.) 

1.  Lee, S.Y., Mermelstein, L.D., Bennett, G.N., and Papoutsakis, E.T., "Vector construction, transformation, and gene amplification in C.acetobutylicum ATCC 824", Ann. NY Acad. Sci. 665: 39-51 (1992. 10. 13.)