Education

• B.S.  : Department of Chemical Engineering, Chungang University, Seoul, Korea (Mar.1989 – Feb. 1993)

• M.S. : Department of Chemical Engineering, KAIST, Taejon, Korea (Mar. 1993 – Feb. 1995)

• Ph.D.: Department of Chemical Engineering, KAIST, Taejon, Korea (Mar. 1995 – Aug. 1999)

Thesis

•  M.S. Thesis  : Purification of lactic acid using solid catalyzed reaction (Supervised by Won Hi Hong)

• Ph.D. Thesis : Development of recombinant Escherichia coli strain and the process for the production of poly(3-hydroxyalkanoate)   (Supervised by Sang Yup Lee)

Career

•  Mar. 1995 - Feb. 1999 : Research and teaching assistant in KAIST

• Aug. 1999 – Mar. 2000 : Postdoctoral associater at BioProcess Engineering Research Institute, KAIST

• Apr. 2000 – Sep. 2001 : Postdoctoral associater at Department of Microbiology, University of Minnesota

• Oct. 2001 – Present : Senior researcher at Samchully Pharmaceutical Co.

Research Work in Ph. D. course

• Molecular cloning

• Construction of recombinant bacteria

• Metabolic engineering

• High cell density culture

• Production and recovery of biodegradable polymer

• Process analysis and economic evaluation

Research Work at Postdoctoral Fellowship

•   Discovery of new anti-cancer drug

• Separation of sponge cells producing bioactive compound from sponge samples

• Construction of BAC library to target the PKS pathway

• Cloning of novel PKS genes from deep marine bacteria

• Discovery of new PKS biosynthesis pathway

Research Work at Postdoctoral Fellowship

• production of pyrimidine deoxyribonucleoside by microorganisms

Publications

• International Journal: 17 papers

• Book Chapter: 4 papers

• International Proceeding: 7 papers

• Domestic Journal: 7 papers

• International Conference: 19

• Domestic Conference: 17

• International Patent: 1

International Journal

• 1. Choi, J., Hong W.H., Chang, H.N. 1995. Reaction kinetics of lactic acid with methanol catalyzed by acid resins. Int. J. Chem. Kinetics, 28: 37-41.

• 2. Choi, J., Lee S.Y. 1997. Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. Bioprocess Eng., 17(6): 335-342.

• 3. Lee, S.Y., Choi, J. 1998. Effect of fermentation of performance on economics of poly(3-hydroxybutyrate) production by Alcaligenes latus. Polymer Degrad. Stabil., 59: 387-393.

• 4. Choi, J., Lee, S. Y., Han, K. 1998. Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of poly(3-hydroxybutyrate) by recombinant Escherichia coli. Appl. Environ. Microbiol., 64: 4897-4903.

• 5. Choi, J., Lee, S. Y. 1999. Efficient and economical recovery of poly(3-hydroxybutyrate) from recombinant Escherichia coli by simple digestion with chemicals. Biotechnol. Bioeng., 62(5): 546-553.

• 6. Lee, S. Y., Choi, J. 1999. Production and degradation of polyhydroxyalkanoates in waste environment. Waste Management, 19: 133-139.

• 7. Choi, J., Hong, W. H. 1999. Recovery of lactic acid by batch distillation with chemical reactions using ion exchange resin. J. Chemical Eng. Japan, 32(2): 184-189.

• 8. Choi, J., Lee, S. Y. 1999. Factors affecting the economics of polyhydroxyalkanoates production by bacterial fermentation. Appl. Microbiol. Biotechnol., 51(1): 13-21.

• 9. Choi, J., Wong, H. H. 1999. Recent advances in polyhydroxyalkanoate production by bacterial fermentation. Intl. J. Biol. Macromol. 25: 31-36.

• 10. Lee, S. Y., Choi, J., Han, K., Song, J. Y. 1999. Removal of endotoxin during the purification of poly(3-hydroxybutyrate) from gram-negative bacteria. Appl. Environ. Microbiol., 65(6): 2762-2764.

• 11. Choi, J., Lee, S. Y. 1999. Production of poly(3-hydroxybutyrate) [P(3HB)] with high P(3HB) content by recombinant Escherichia coli harboring the Alcaligenes latus P(3HB) biosynthesis genes and the E. coli ftsZ gene. J. Microbiol. Biotechnol., 9(6):722-725.

• 12. Wong, H. H., van Wegen R. J., Choi, J., Lee, S. Y., Middelberg, A. P. J. 1999. Metabolic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli. J. Microbiol. Biotechnol., 9(5):593-603.

• 13. Choi, J., Lee, S. Y. 1999. High level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli. Appl. Environ. Microbiol., 65(10): 4363-4368.

• 14. Lee, S. H., Oh, D. H., Ahn, W. S., Lee, Y., Choi, J., Lee, S. Y. 2000. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high cell density cultivation of Aeromonas hydrophila. Biotechnol. Bioeng., Biotechnol. Bioeng., 67(2): 240-244.

• 15.Choi, J. and Lee, S.Y. 2000. Economic consideration on the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacterial fermentation. Appl. Microbiol. Biotechnol., 53(6): 646-649.

• 16. Lee, S. Y., Wong, H. H., Choi, J., Lee, S. H., Lee, S. C., Han, C. S. 2000. Production of medium-chain-length polyhydroxyalkanoates by high cell density cultivation of Pseudomonas putida. Biotechnol. Bioeng., 68(4):466-470.

• 17. Lee, S. Y., Choi, J. 2001. Production of microbial polyester by fermentation of recombinant microorganisms. Adv. Biochem. Eng. Biotechnol., 71:183-207.

Book Chapter

• 1. Lee, S.Y., Choi, J. 1999. Chapter 51. Polyhydroxyalkanoate: biodegradable polymer. In : Manual of Industrial Microorganisms and Biotechnology, 2nd ed., eds. A. L. Demain and J. E. Davies, American Society for Microbiology, Washington DC. USA, pp. 616-627.

• 2. Lee, S. Y., Choi, J. 1999. Metabolic engineering strategies for the production of polyhydroxyalkanoates, a family of biodegradable polymers. eds. S. Y. Lee and E. T. Papoutsakis. Marcel Dekker, USA, pp. 113-151.

• 3. Lee, S.Y., Choi, J. 2000. Polyhydroxyalkanoate production by recombinant Escherichia coli: new genes and new strains, In: ACS symposium series 764, Polymers from renewable resources - Biopolyesters and biocatalysis. eds. C. Scholz, R. A. Gross, American chemical society. Washington DC, USA, pp. 77-88.

• 4. Lee, S.Y.Choi, J. 2001. Production of microbial polyesters by fermentation of recombinant microorganisms, In: Biopolyesters eds. W. Babel and A. Steinbuchel, Springer Velag, Berlin, Germany pp. 183-207.

International Proceeding

• 1. Lee, S.Y., Choi, J., Chang, H.N. 1997. Process development and economic evaluation for the production of polyhydroxyalkanoates by Alcaligenes eutrophus. Proceedings of 96 International Symposium on Bacterial Polyhydroxyalkanoates. Swiss, Davos, 127-136.

• 2. Choi, J., Lee, S.Y. 1997. Development of recombinant Alcaligenes eutrophus for the efficient production of poly(3-hydroxybutyrate). Proceeding of 4th APBioChEC’97, Beijing, China, 626-629.

• 3.Choi, J., Wang, F., Lee, S.Y. 1997. Production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinant Escherichia coli in defined medium. Proceeding of 4th APBioChEC’97, Beijing, China, 630-633.

• 4. Lee, S. Y., Choi, J., Wang, F. 1998. Production of poly(3-hydroxybutyrate) by recombinant bacteria. In Science and Technology of Polymers and Advanced Materials. Proceedings of the 4th International conference on Frontiers of Polymers and Advanced Materials, Cairo, Egypt, eds. P. N. Prasad et al., Plenum Press, New York, 463-475.

• 5. Lee, S.Y., Choi, J. 1998. Metabolic engineering of Escherichia coli for the production of polyhydroxyalkanoates. Proceeding of the 7th International Conference on Computer Application in Biotechnology, Osaka, Japan, 363-377.

• 6. Lee, S.Y., Choi, J., Lee, S. H. 1999. Production of polyhydroxyalkanoates by fermentation of bacteria. Proceeding of 7th SPSJ International Polymer Conference, Yokohama, Japan.

• 7. Choi, J., Hong, W. H., Lee, S. Y. 1999. Recovery of poly(3-hydroxybutyrate) from recombinant Escherichia coli. Proceedings of 5th APBioChEC’99. Phuket, Thailand.

Domestic Journal

• 1. Lee, S.Y., Lee, Y., Choi, J. 1995. Production and application of polyhydroxyalkanoates. Kor. J. Biotechnol. Bioeng., 10(4): 406-420.

• 2. Choi, J., Lee, S.Y. 1995. Polyhydroxyalkanoates. Recent Advances in Bioprocess Engineering, 3: 3-16.

• 3.Choi, J., Lee, S. Y. 1996. Production of polyhydroxyalkanoates by recombinant microorganisms: Application of metabolic engineering. Bioindustry News, 9(4): 47-55.

• 4. Hur, S.S., Choi, J., Lee, S.Y. 1997. Isolation of Poly(3-hydroxybutyrate) [PHB] accumulating bacteria from soil and production of PHB from inexpensive carbon substrates, Kor. J. Biotechnol. Bioeng., 12: 451-458.

• 5. Choi J., Lee, S.Y. 1997. Development of recombinant Alcaligenes eutrophus for the production of poly(3-hydroxybutyrate). Hwahak Konghak, 35: 684-689.

• 6. Lee, S. Y., Choi, J., Wong, H. H., Wang, F. 1998. Production of bioproducts by high cell density culture of recombinant Escherichia coli. Biochemical Eng., 12: 3-13.

• 7.Choi, J., Hong, W. H. 1999. Separation characteristics of lactic acid by batch reactive distillation. Kor. J. Biotechnol. Bioeng., 14(2): 220-224.

International Conference

• 1. Choi, J., Hong, W.H. Distillation of lactic acid using its ester. 7th Chungnam-Kyushu International Symposium 1994, Kitakyushu, Japan, November (1994).

• 2. Lee, S.Y., Choi, J. Enhancement of PHB synthesis by suppressing filamentation in recombinant Escherichia coli. International Symposium on Biodegradable Polymers, Tokyo, Japan, November (1995).

• 3. Lee, S.Y., Choi, J. Characterization of filamentation-suppressed recombinant E. coli. 8th Chungnam-Kyushu International Symposium 1995, Taejon, Korea, December (1995).

• 4. Lee, S.Y., Choi, J., Chang, H.N. Optimization and economics of polyhydroxyalkanoate production in bacteria. International Symposium on Bacterial Polyhydroxyalkanoates '96, Davos, Switzerland, August (1996).

• 5. Lee, S. Y., Choi, J., Lee. Y. Polyhydroxyalkanoates synthesis by metabolically engineered Escherichia coli strains. Recombinant DNA Biotechnology IV: Metabolic Engineering, Danover, MA, U.S.A., October (1996).

• 6. Jeong, K.J., Choi, J., Lee, S.Y. Construction of shuttle vector between Escherichia coli and Alcaligenes sp. 9th Chungnam-Kyushu International Symposium. Miyazaki, Japan, October (1996).

• 7. Choi, J., Lee, S.Y. Development of recombinant Alcaligenes eutrophus for the efficient production of poly(3-hydroxybutyrate). 4th APBioChEC’97, Beijing, China, October (1997).

• 8. Choi, J., Wang, F., Lee, S.Y. Production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinant Escherichia coli in defined medium. 4th APBioChEC’97, Beijing, China, October (1997).

• 9. Choi, J., Wang, F., Lee, S.Y. Production of poly(3-hydroxybutyrate) with high productivity and high P(3HB) content by fed-batch culture of Alcaligenes latus under nitrogen limitation. 10th Chungnam-Kyushu International Symposium. Taejon, Korea, November (1997).

• 10. Lee, S.Y., Choi, J. Metabolic engineering of Escherichia coli for the production of polyhydroxyalkanoates. 7th International Conference on Computer Application in Biotechnology, Osaka, Japan, June (1998).

• 11.Choi, J., Lee, S. Y. Cloning and molecular analysis of polyhydroxyalkanoate biosynthesis genes of a gram negative bacterium. 7th Annual Meeting of the Bio/Environmentally degradable Polymer Society, Boston, USA, August (1998).

• 12.Choi, J., Lee, S. Y. Design and analysis of Process for the production of poly(3-hydroxybutyrate) by recombinant Escherichia coli. 7th Annual Meeting of the Bio/Environmentally degradable Polymer Society, Boston, USA, August (1998).

• 13.Choi, J., Lee, S. Y. Production of poly(3-hydroxybutyrate) with high productivity by fed-batch culture of recombinant Escherichia coli harboring the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes. 7th Annual Meeting of the Bio/Environmentally degradable Polymer Society, Boston, USA, August (1998).

• 14. Lee, S. Y., Choi, J., Wong H. H. Poly(3-hydroxybutyrate) production from whey by recombinant Escherichia coli. 7th Annual Meeting of the Bio/Environmentally Degradable Polymer Society, Boston, USA, August (1998).

• 15. Choi, J., Lee, S. Y. Efficient recovery of poly(3-hydroxybutyrate) from recombinant Escherichia coli by simple digestion with chemicals. International Symposium on Biological Polyhydroxyalkanoates 98, Tokyo, Japan, September (1998).

• 16. Choi, J. Poly(3-hydroxybutyrate) production from xylose. International Symposium on Biological Polyhydroxyalkanoates 98, Tokyo, Japan, September (1998).

• 17.Choi, J., Lee, S. Y. Economic recovery of poly(3-hydroxybutyrate) from recombinant Escherichia coli by simple digestion. 12th Chungnam-Kyushu International Symposium. Kongju, Korea, October (1999).

• 18. Ahn, W. S., Choi, J., Lee, S. Y. Poly(3-hydroxybutyrate) production by recombinant Escherichia coli harboring the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes. 12th Chungnam-Kyushu International Symposium. Kongju, Korea, October (1999).

• 19. Choi, J., Hong, W. H., Lee, S. Y. Recovery of poly(3-hydroxybutyrate) from recombinant Escherichia coli. Proceedings of 5th APBioChEC’99. Phuket, Thailand.(1999)

Domestic Conference

• 1. Choi, J., Hong, W.H. Purification of lactic acid by esterification reaction. KIChE Fall Meeting, Chonbuk Univ., October (1994).

• 2. Midelberg, A.P.J., Lee, S.Y., Choi, J. Study on the poly(3-hydroxybutyric acid) granules produced in recombinant E. coli. Annual Meeting of Korean Society for Applied Microbiology, Seoul National Univ., April (1995).

• 3. Lee, S.Y., Choi, J. Production of medium chain length poly-(3-hydroxyalkanoates) by Pseudomonas oleovorans. Annual Symposium of KIBB, SunMun Univ., October (1995).

• 4. Choi, J., Lee, S.Y. Economic evaluation for poly(3-hydroxybutyrate) production by bacterial fermentation. Annual Symposium of KIBB, Pusan National Univ., October (1996).

• 5. Choi, J., Lee, S. Y. Production of PHB by recombinant Alcaligenes eutrophus. KIChE Spring Meeting, Dongkuk Univ., April (1997).

• 6. Wang, F., Choi, J., Park, S.J., Lee, S.Y. Production of poly(3-hydroxybutyrate) with high productivity and high P(3HB) content by fed-batch culture of Alcaligenes latus under nitrogen limitation. KIChE Fall Meeting, Chungnam Univ., October (1997).

• 7. Choi, J., Lee, S.Y. Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. Annual Symposium of KIBB, Chonbuk Univ., April (1998).

• 8. Choi, J., Lee, S.Y., Isolation of poly-3-hydroxybutyrate [P(3HB)] accumulating bacteria from soil and production of P(3HB) from inexpensive carbon substrates, Annual Meeting of Korean Society for Applied Microbiology, Korea Univ., April (1998).

• 9. Choi, J., Lee, S. Y., Process analysis for polyhydroxyalkanoate production, Annual BPERC Symposium’98, KAIST. September (1998).

• 10. Choi, J., Lee, S. Y., Production of polyhydroxyalkanoate by new recombinant Escherichia coli, Annual BPERC Symposium’98, KAIST. September (1998).

• 11. Lee, S. Y., Choi, J., Wong H. H. Poly(3-hydroxybutyrate) production from whey by recombinant Escherichia coli. Annual BPERC Symposium’98, KAIST. September (1998).

• 12. Choi, J., Lee, S. Y. Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for the enhanced production of poly(3-hydroxybutyrate) in Escherichia coli. Annual Symposium of KIBB, Hanyang Univ., October (1998)

• 13. Choi, J., Lee, S. Y., Wong H. H. Poly(3-hydroxybutyrate) production from whey by recombinant Escherichia coli. KIChE Fall Meeting, Chosun Univ., October (1998).

• 14. Choi, J., Lee, S. Y., Chang, H. N. Efficient and economical recovery of poly(3-hydroxybutrate) from recombinant Escherichia coli. Annual Symposium of KIBB, Chonnam Univ., April (1999).

• 15. Choi, J., Lee, S. Y., Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Fed-batch Culture of Recombinant Escherichia coli, KIChE Fall Meeting, Kyungnam Univ., October (1999).

• 16. Choi, J., Lee, S. Y., Endotoxin removal during the recovery of poly(3-hydroxybutyrate), Annual Meeting of Korean Society for Applied Microbiology, Youngnam Univ., October (1999).

• 17. Choi, J., Park, S.J., and Lee, S.Y.: Production of Poly(3-hydroxybutyrate) with high molecular mass by recombinant Escherichia coli. KJChE meeting, Hanyang Univ., Ansan April (2000).

Research Interest

• Protein engineering (protein secretion, protein folding, directed evoloution)

• Recombinant protein production

• High-cell density cultivation

• Proteomics

Education

• 1990. 3. - 1994. 2. Dept. of Biological Technology, KAIST, Daejeon, Korea (B.S.)

• 1994. 3. - 1996. 2. Dept. of Biological Sciences, KAIST, Daejeon, Korea (M.S.)

• 1996. 3. - 2001. 2. Dept. of Chemical Engineering, KAIST, Daejeon, Korea (Ph.D.)

Experience and Academic/Research Activity

•  Teaching and Research Assistant at the department of Chemical Engineering, KAIST (1996. 9 - 1997. 6)

• Member, Korean Society for Applied Microbiology (1997 - present)

• Member, The Biochemical Society of the Republic of Korea (2000 - present)

• Member, Korea Institute of Biotechnology and Bioengineering (2000 - present)

• Senior researcher, Dept. Chemical Engineering KAIST (2001. 3 - 2002. 9.)

• Post-doc, Dept. of Chemical Engineering, University of Texas at Austin, USA (2002. 10. - present)

Publications

•  International Journals

• Jeong K. J., Lee P. C., Park I. Y., Kim M. S. and Kim S. C., "Molecular cloning and characterization of an endoxylanase gene of Bacillus sp. in Escherichia coli", Enzyme Microb Technol. 22(7): 599-605 (1998). [Abstract]

• Jeong K. J., Park I. Y., Kim M. S. and Kim S. C., "High-level expression of an endoxylanase gene from Bacillus sp. in Bacillus subtilis DB104 for the production of xylobiose from xylan", Appl. Microbiol. Biotechnol. 50(1): 113-118 (1998). [Abstract]

• Jeong, K. J., Lee, H. S., Lee, S. Y., and Chang Y. K.,  "Development of recombinant bacteria for the degradation of dibenzothiophene", Ann. NY Acad. Sci., 864:375-378 (1998).

• Jeong. K. J. and Lee, S. Y.,  "High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification", Appl. Environ. Microbiol. 65(7):3027-3032 (1999). [Abstract]

• Jeong. K. J. and Lee, S. Y.,  "Secretory production of human leptin in Escherichia coli", Biotechnol. Bioengin. 67:398-407 (2000). [Abstract]

• Choi, J. H., K. J. Jeong, S. C. Kim and S. Y. Lee., "Efficient secretory production of alkaline phosphatase by high cell density culture of recombinant Escherichia coli using the Bacillus sp. endoxylanase signal sequence", Appl. Microbiol. Biotechnol. 53:640-645 (2000). [Abstract]

• Jeong, K.J. and Lee, S.Y.,  "Secretory production of human leptin in Bacillus subtilis??J. Microbiol. Biotechnol. 10(6):753-758 (2000).

• Jeong, K. J. and S. Y. Lee, "Secretory production of human granulocyte colony stimulating factor in Escherichia coli",  Protein Express. Purif. 23:311-318 (2001).

• Lim S. C., K. J. Jeong, H. N. Chang and S. Y. Lee, "High-level secretory production of human granulocyte-colony stimulating factor by fed-batch culture of recombinant Escherichia coli", Bioprocess Biosystems Engineering, 24:249-254 (2001).

• Jeong, K.J., and Lee, S.Y.,(2002) "Excretory Production of Human -Endorphin into Culture Medium by Using Outer Membrane Protein F as a Fusion Partner in Recombinant Escherichia coli ", Appl. Environ. Microbiol.,68(10):4979-4985 (2002). 

• Yoon,S.H., Han, M.J., Lee, S.Y., Jeong, K.J., and Yoo, J.S., "Combined transcriptome and proteome analysis of Escherichia coli during the high cell density culture" Biotechnol. Bioeng., 81(7) : 753-767 (2003). 

• Jeong, K.J. and Lee, S.Y., "Enhanced production of recombinant proteins in Escherichia coli by filamentation suppression", Appl. Environ. Microbiol. 69(2) : 1295-1298 (2003).

• Han, M.J., Jeong, K.J., Yoo, J.S, and Lee. S.Y., "Engineering Escherichia coli for increased production of serine-rich proteins based on proteome profiling", Appl. Environ. Microbiol., 69(10) : 5772-5781 (2003).

• Chen, S., Huang, X., Zhou, X., Bai, L., Jing He, Jeong, K. J.,Lee, S.Y., and Deng, Z., Chemistry and Biology, in press

• Lee, S.Y. and Jeong, K. J.,  "Cellular engineering of Escherichia coli for the enhanced protein production by fermentation", in Fermentation Biotechnology (ed. Saha, B.C.), American Chemical Society, Washinton, D.C., USA (2004).

• Domestic Journals

• Ki Jun Jeong, Hyun Sook Lee, Sang Yup Lee and Yong Keun Chang, "Efficient transformation of Klebsiella oxytoca by electroporation", Biotechnol. Bioprocess Eng., 2. 87-91 (1998).

• Ki Jun Jeong, and Sang Yup Lee, "Secretory production of human leptin in Bacillus subtilis", J. Microbiol. Biotechnol., 10(6):753-758 (2000).

Invited Lectures

• Jeong, K. J., "Secretory production of proteins in E. coli : focus on excretion to the culture medium", Biochemical Engineering XII, Sonoma, California, USA, June (2001)

International Conferences

•  Jeong K. J., Choi J. I., and Lee S. Y., "Vector construction, transformation, and gene ampilification in Alcaligenes latus", The 9th Kyushu/Chungnam Symposium on Chemical Engineering, Kushu, Japan (1996).

• Jeong K. J. Lee, H. S., Lee S. Y., Chang, Y. K., "Development of recombinant Klebsiella oxytoca for the desulfurization activity", The 10th Kyushu/Chungnam Symposium on Chemical Engineering, Taejon, Korea (1997).

• Lee, S. Y., Jeong, K. J., Lee, H. S., and Chang Y. K., "Development of recombinant bacteria for the degradation of dibenxothiophen", Enzyme Engineering XIV CONFERENCE, Beijing, China (1997).

• Jeong, K. J. and Lee, S. Y.,  "Efficient secretion of protein in Escherichia coli using endoxylanase signal sequence of Bacillus subtilis", Metabolic Engineering II, Elmau/Oberbayern, Germany (1998).

• Lee, S.Y., and Jeong, K.J., "High level production of human leptin and its purification", The Microfluidics & Microarrays Conference : Small Talk '99 San Diego, CA. USA, June (1999).

• Jeong, K. J. and Lee, S. Y.,  "Secretory production of human leptin in Escherichia coli", The 12th Kyushu/Chungnam Symposium on Chemical Engineering, Kongju Univ, Kongju (1999).

• Jeong, K. J. and Lee, S. Y., "Secretory production of leptin in Escherichia coli" The 5th Asia-Pacific Biochemical Engineering Conference 1999 and The 11th Annual Meeting of The Thai Society for Biotechnology, Phuket, Thailand (1999).

• Lee, S.Y., and Jeong, K. J., "High level production of human leptin and its purification", Biochemical Engineering XI, Park city, Utah, July (1999)

• Lee, S. Y., Yoon, S. H., Han, M. J., Jeong, K.J., "Combined analysis of transcriptome and proteome profiles during the high cell density cultivation of Escherichia coli", Biochemical Engineering XII, Sonoma, California, USA, June (2001)

• Han, M.J., Choi, J.H., Jeong, K.J., Yoo, J.S. and Lee, S.Y.,  "Proteome Analysis of Overproducing Human Leptin System", The 14th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, Chungnam Univ., Taejon , Korea (2001)

Domestic Conferences

• Jeong K. J. and Kim S. C.,  "Molecular cloning and sequencing of an endoxylanase gene from Bacillus sp. and their expression in Bacillus subtilis", at the Autumn Meeting of Korea Society for Applied Microbiology (1995).

• Jeong K. J., Lee Sang Yup, and Chang Y. K., "Construction of stable plasmid for the expression of desulfurization gene in E. coli", Annual BPERC Symposium 1996, KAIST, Taejon, Korea (1996).

• Nam S. W., Kim J. H., Jeong K. J., Kim S. C., and Kim J. H.,  "Media optimization for xylanase overproduction by recombinant Bacillus subtilis", at the Autumn Meeting of Korea Society for Applied Microbiology (1996).

• Jeong K. J., Lee S. Y., and Chang Y. K.,  "Construction of stable plasmid for the expression of desulfurization gene in E. coli", at the Spring Meeting of Korea Society for Applied Microbiology (1997).

• Wong Heong Ho, Jeong Ki Jun, Lee Sang Yup,  "Production of Bioadhesive Protein by Fed-batch culture of Recombinant Escherichia coli", Annual BPERC Symposium 1997, KAIST, Taejon, Korea (1997).

• Jeong, K. J, and Lee, S. Y.,  "Overproduction of recombinant leptin in Escherichia coli", Annual BPERC Symposium 1998, KAIST, Taejon, Korea (1998).

• Jeong, K. J., Choi, J. H. and Lee, S. Y., "Efficien secretion of recombinant endoxylanase in Escherichia coli", Annual BPERC Symposium 1998, KAIST, Taejon, Korea  (1998).

• Jeong, K. J, and Lee, S. Y., "Fed-batch fermentation of a recombinant Escherichia coli overproducing human leptin", at the Autumn Meeting of Korea Society for Applied Microbiology (1998).

• Park, S. J., Jeong K. J., Lee, H. S., Lee S. Y., and Chang Y. K.,  "Development of recombinant bacteria for the degradation of dibenzothiophen", KJChE fall meeting, Chosun Univ., Kwangju (1998).

• Choi, J.H., Jeong, K.J., Lee, S.Y.,  "Construction of secretion Vector containing Endoxylanase Signal Sequence from Bacillus subtilis and Secretory", Production of Alkaline Phosphatase in Escherichia coli", Annual Symposium of Korea Institute of Biotechnology and Bioengineering (KIBB) Chunnam Univ., Kwangju (1999).

• Jeong, K. J., Lee, S.Y.,  "Overproduction of Human Leptin by Fed-Batch Cultivation of Recombinant Escherichia.coli and its purification", At the Spring Meeting of The Korean Society for Applied Microbiology, SeoGang Univ, Seoul (1999).

• Jeong, K. J., Lee, S.Y.,  "Secretory production of human leptin in Escherichia coli", At the Autumn Meeting of The Korean Society for Applied Microbiology, Youngnam Univ, Ulsan (1999).

• Jeong, K. J., and Lee, S. Y., "Production of human leptin in Bacillus subtilis", Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 535-538. (2000, Apr.)

• Jeong, K. J., and Lee, S. Y., "Production of soluble human leptin in periplasmic space of Escherichia coli using DsbA coexpression", Theories and Applications of Chemical Engineering 6(1): 1197-2000 (2000).

• Jeong, K. J., and Lee, S. Y., "Production of soluble human leptin in periplasmic space of Escherichia coli using DsbA coexpression KJChE meeting", Hanyang Univ., Ansan April, 2000

• Jeong, K. J., Lee, S. Y., "Secretory production of human granulocyte colony stimulation factor in Escherichia coli", Annual Meeting of The Korean Society for Applied Microbiology, Cheon-buk Univ., October, 2000

• Lim, S. C., Jeong, K. J., Chang, H. N., Lee, S. Y., "Secretory production of human G-CSF in E. coli by large-scale fermentor", Annual meeting of Korean society for Biotechnology and Bioengineering , Konkuk Univ., Seoul, April (2001)

• Yoon, S.H., Han, M.J., Jeong, K. J., Yoo, J.S., Lim, B.K., Lee, S.Y., "Transcriptome and proteome analysis of Escherichia coli during high cell density cultivation", Annual Meeting of Korean Society for Applied Microbiology, Chonan Sangnok Resort, June(2001)

• Jeong, K. J., Lee, S. Y., "Secretory production of Human Granulocyte- colony stimulating factor into periplasm of Escherichia coli and its purification, Annual Meeting of Korean Society for Applied Microbiology", Chonan Sangnok Resort, June (2001)

• Han, M.J., Choi, J.H., Jeong, K.J., Yoo, J.S. and Lee, S.Y., "Proteome Analysis of Overproduction of Recombinant Protein in Escherichia coli by Fed-Batch Fermentations", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, Seoul, November(2001)

• Han, M.J., Choi, J.H., Jeong, K.J., Yoo, J.S. and Lee, S.Y., "Proteome Analysis of Overproduction of Human Leptin in Escherichia coli by Fed-Batch Fermentations", KIChE meeting, Taejon , Korea (2001)

• Jeong, K.J. and Lee, S.Y., "Excretory production of human β-endorphin into culture medium by recombinant Escherichia coli", KIChE meeting, Taejon , Korea (2001)

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Research Interest

• PolyHydroxyAlkanoate(PHA) monomer production/modification/application

• Recombinant protein production

Education

• 1992 (B.S.) Dept. of Chemical Engineering, KAIST, Taejon, Korea

• 1997 (M.S.) Dept. of Chemical Engineering, KAIST, Taejon, Korea

• 2002 (Ph.D.) Dept. of Chemical & Biomolecular Engineering, KAIST, Taejon, Korea

Experience and Academic Activity

• Researcher, BioProcess Engineering Research Center (1994-2002)

• Member, Korean Institute of Chemical Engineers (1995-present)

• Member, Korean Society for Biotechnology and Bioengineering (1995-present)

• CEO, ChiroBio Inc., Taejon, Korea (2000-present)

Honor/Awards

• The First Elmer Garden Award - coauthor, Biotechnology and Bioengineering (John Wiley & Sons, USA) – American Chemical Society (San Francisco National Meeting) (2000.3.27)

• Award for Excellent Poster, Korean Society for Biotechnology and Bioengineering (2000.11.10)

• SejongDaeWang Award – The Best Patent Award, KIPO (2001.3)

Publications

•  International Journal

• 1.   Lee, Y. and Lee, S.Y.  "Enhanced production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinant Escherichia coli in a defined medium", J. Environ. Polymer Degrad. 4: 131-134 (1996)

• 2.   Lee, Y., Lee, S.Y. and Yang, J. "Production of rhamnolipid biosurfactant by fed-batch culture of Pseudomonas aeruginosa using glucose as a sole carbon source", Biosci. Biotechnol. Biochem. 63(5): 946-947(1999)

• 3.   Lee, S.Y., Lee, Y., and Wang, F. "Chiral compounds from bacterial polyesters: Sugars to plastics to fine chemicals" Biotechnol. Bioeng.. 65:363-368(1999)

• 4.   Lee, Y., Park, S.H., Lim, I.T., Han, K. and Lee, S.Y. "Preparation of alkyl (R)-(-)-3-hydroxybutyrate by  acidic alchoholysis of poly-(R)- (-)-3-hydroxybutyrate" Enzyme Microb. Technol. 27:33-36 (2000).  

• 5.  Lee, S.H., Oh, D.H., Ahn, W.S., Lee, Y., Choi, J., and Lee, S.Y., "Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high cell density cultivation of Aeromonas hydrophila" Biotechnol. Bioeng. 67:240-244(2000)

• 6.  Lee, Y., Park, S.H., Lim, I.T., Han, K. and Lee, S.Y., "Preparation of alkyl (R)-(-)-3-hydroxybutyrate by acidic alchoholysis of poly-(R)- (-)-3-hydroxybutyrate" Enzyme Microb. Technol. 27:33-36 (2000).

• 7.  Lee, S.Y. and Lee, Y., "Metabolic engineering of Escheichia coli for production of enantiomenically pure (R)-(-)-hydroxycarboxylic acids", Appl. Environ. Microbiol., 69(6) : 3421-3426 (2003).

• 8.  Park, S.J., Lee, Y., and Lee, S.Y. "Biosynthesis of (R)-3-hydroxyalkanic acid by metabolically engineered E.coli" Appl. Biochem. Biotechnol., In press.

• International Proceedings

• 1.    Lee, Y., Lim, I.-T., Han, K., and Lee S.Y. "Preparation of Enatiomerically pure hydroxycarboxylic acid"CD ROM (No. P-IB15) Proceedings of APBioCheC "9, Phuket, Thailand (1999) 

• Korean Journal

• 1.    Lee, S.Y., Lee, Y., and Choi, J. "Production and application of polyhydroxyalkanoates", Kor. J. Biotechnol. Bioeng., 10(4): 406-420 (1995).

• 2.   Lee, Y., Lee, S.Y., Yang, J.W., and Park, C.H. "Biosurfactants", Biochemical Eng. 10: 53-63 (1996).

• 3.   Lee, Y., Lee, S.H., Lee, S.Y."Fed-batch culture of Aeromonass hydrophila for the production of poly(3-hydroxybutyrate-co-hydroxyhexanoate) using two carbon sources" Biotechnol.Bioproc. Eng. 4:195-198(1999)

• Korean Proceedings

• 1.    Lee, S.Y. and Lee, Y. "PHB synthesis by filamentation suppressed recombinant E. coli", Proceedings of BPERC International Symposium '95, p. 181-182 (1995).

• 2.   Lee, S.Y., Lee, Y., and Chang, H.N. "High cell density culture of recombinant E. coli for the production of PHB", Proceedings of BPERC International Symposium '95, p. 183-184 (1995).

• 3.   Lee, Y. and Lee, S.Y. "Enhancement of production of poly(3-hydroxybutyrate) by using filamentation-suppressed recombinant E. coli in a defined medium", Theories and Applications of Chemical Engineering 2(1): 493-496 (1996).

• 4.   Lee, Y. and Lee, S.Y. "Production of Optically Pure Monomer of PHB and It's Methyl-, Ethyl-esters by using Acidic degradation of PHB", proceedings of BPERC International Symposium '97, 149-150 (1997).

• 5.  Lee, Y. and Lee, S.Y. "Production of optically pure[D]-hydroxybutyric acid and it's methyl-, ethyl-esters by chemical depolymerization of PHB", Theories and Applications of Chemical Engineering, 2361-2364 (1997)

• 6.   Lee, Y., Lee, S.Y., Han, K.B., and Lim, I.T. "Production of optically pure methyl (D)-3-hydroxybutyrate by acidic depolymerization of microbial polymer, polyhydroxybutyrate(PHB)", Theories and Applications of Chemical Engineering, 1217-1220 (1998)

• 7.   Lee, Y., Lim, I.T., Han,K.B., Lee, S.Y.  " Preparation of optically acive alkyl (R)-(-)-3-hydroxybutyrate by chemical depolymerization of Polyhydroxyalkanoate"Proceedings of Korea Institute of Biotechnology and Bioengineering Symposium, 106-109 (1999)

• 8.    Lee, Y., and Lee, S.Y.  "Preparation of enantiomerically pure (R)-3-hydroxybutyrate"Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 579-582 (2000, Apr.)

• 9.  Lee, Y., Kang, K.H. and Lee, S.Y. "Production of enantiomerically pure (R)-3-hydroxybutyric acid using metabolically engineered Escherichia coli" Theories and Applications of Chemical Engineering 7(2): 4039-4042 (2001)

• International Conferences

• 1.    Lee, S.Y. , Lee, Y., Chang, Y.K., and Chang, H.N. "Enhanced synthesis of poly(3-hydroxybutyrate) by filamentation-suppressed recombinant E. coli", Pacifichem-95, Honolulu, Hawaii, U.S.A., December (1995).

• 2.   Lee, S.Y., Lee, Y., Park, S., Lee, S.H., "Production of (R)-hydroxycarboxylic acids by bacteria" ISBP/BEDPS 2000, Honolulu, Hawaii, December (2000)

• 3.   Park, S.H., Lee, S.H., Lee, Y., Lee, S.Y., "Preparation of optically active b-amino acids from microbial polymer" 221st National meeting of American Chemical Society" San Diego,CA, USA, April (2001)

• Korean Conferences

• 1.   Lee, S.Y., Lee, Y., and Chang, H.N., "Comparison of PHB synthesis depending on the E. coli strain characteristics", '94 BPERC International Symposium, KAIST, September (1994).

• 2.   Lee, S.Y., Lee, Y., and Chang, H.N. "Production of PHB by fed-batch culture of recombinant E. coli in a defined medium containing small amounts of amino acids", Annual Meeting of KIChE, ChunBuk Univ., October (1994).

• 3.   Lee, S.Y., Lee, Y., and Chang, H.N. "High cell density culture and production of PHB by recombinant E. coli using sucrose as a carbon source", Annual Meeting of KIBB, Inha Univ., October (1994).

• 4.   Lee, S.Y., Lee, Y., and Chang, H.N. "Effect of growth temperature and nutrients on the synthesis of poly(3-hydroxybutyric acid) by filamentation-suppressed recombinant E. coli", Annual Meeting of KIBB, KAIST, April (1995).

• 5.   Chung, Y.L., Lee, Y., Yang, J.W., Lee, S.Y., and Park, C.H. "Screening of microorganisms producing emulsifiers", Annual Meeting of Korean Society for Applied Microbiology, Seoul National Univ., April (1995).

• 6.   Lee, Y., Lee, S.Y., and Chang, H.N. "Production of PHB by high cell density cultivation of filamentation-suppressed recombinant E. coli", Annual Meeting of Korean Society for Applied Microbiology, MyungJi University, YongIn, October (1995).

• 7.   Lee, Y. and Lee, S.Y.  "Production of PHB by filamentation-suppressed recombinant E. coli", ChungNam-Kyushu International Symposium 1995, ChungNam Univ., Taejon, December (1995).

• 8.   Lee, Y. and Lee, S.Y.  "Enhanced production of PHB by filamentation -suppressed recombinant E. coli in a defined medium", Annual Symposium of KIChE, KOEX, Seoul, May (1996).

• 9.   Lee, Y. and Lee, S.Y.  "Cloning of Pseudomonas aeruginosa YPJ80 rhamnolipid byosynthesis genes", ibid (1996).

• 10.  Lee, Y. and Lee, S. Y. " Efficient production of biosurfactant by fed-batch culture of Pseudomonas aerusinosas YPJ-80", Annual Symposium of KIBB, MyungJi Univ. Yongin, April (1997)

• 11.  Lee, Y. and Lee, S.Y.  "Production of optically pure (D)-hydroxybutyric acid and it's methyl-, ethyl-esters by chemical depolymerization of PHB", Annual Symposium of  KIChE, ChungNam Univ., October (1997)

• 12.   Lee, Y., Lee, S.Y. , Han, K.B., and Lim, I.T. "Production of optically pure methyl (D)-3-hydroxybutyrate by acidic depolymerization of microbial polymer, polyhydroxybutyrate(PHB)", Annual Symposium of KIChE, KOEX, Seoul, April (1998)

• 13.   Lee, Y. and Lee, S.Y.  "Efficient production of biosurfactant by fed-batch culture of Pseudomonas aeruginosa YPJ-80", A Day's Workshop for New Surfactants, KAIST, Taejon, Korea, July (1998)

• 14.   Lee, Y., Lim, I.-T., Han, K., and Lee, S.Y. "Preparation of optically active alkyl (R)-(-)-3-hydroxybutyrate by chemical depolymerization of Polyhydroxyalkanoate"Annual Symposium of Korea Institute of Biotechnology and Bioengineering (KIBB). Chunnam Univ., Kwangju (1999)

• 15.  Lee, Y., and Lee, S.Y.  "Production of Optically active (R)-(-)-Hydroxycarboxylic acid"Annual Meeting of The Korean Society for Applied Microbiology, Youngnam Univ, Taegu (1999).

• 16.  Lee, Y., and Lee, S.Y.  "Preparation of enantiomerically pure (R)-3-hydroxybutyrate"Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, KAIST, April (2000)

• 17.  Lee, S.Y., Park, S.H., Lee, Y., and Lee, S.H. "Metabolic engineering for the production of fine chemicals" Annual Meeting of the Korean Chemical Society , Hanyang Univ., April (2001)

• 18.  Lee, S.Y., Park, S.J. and Lee, Y. "Metabolic Engineering of Escherichia coli for the Production of Novel PHA and Chiral Compounds" Annual Meeting of Korean Society for Applied Microbiology, Chonan Sangnok Resort, June(2001)

• 19.   Lee, Y., Kang, K.H. and Lee, S.Y. "Production of enantiomerically pure (R)-3-hydroxybutyric acid using metabolically engineered Escherichia coli" KIChE meeting, Taejon , Korea (2001)

• Books

• 1. Lee, S.Y., Park, S.H., Lee, Y. and Lee, S.H. "Production of chiral and other valuable compounds from microbial polyesters", Biopolymers Vol 3b, Ch 14, 375-387, VCH (2001)

• 2. Lee, S.Y., Park, S.H., Hong, S.H., Lee, Y. and Lee, S.H. "Fermentative Production of Building Blocks for Chemical Synthesis of Polyesters", Biopolymers Vol 4, Ch 10, 265-325, VCH (2001)

• 3. Lee, S.Y., Park, S.J., Park, J.P., Lee, Y. and Lee, S.H. "Economic aspects of biopolymer production", Biopolymers Vol 10, Ch 2, VCH  in press (2002)

• 4. Lee, S.Y., Park, S.J., Lee, Y. and Lee, S.H. "Biotechnological processes for the production of monomers for subsequent chemical polymer synthesis", Biopolymers Vol 10, Ch 3, VCH  in press (2002)

Professional Experience

• 2009.3 - present   Postdoctorial Fellow, Institute for Systems Biology at Seattle

• 2009.1 - 2009.3    Senior Research Scientist, Industrial Biotechnology and Bioenergy Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea

• 2008  - present     Editorial Advisory Board Member, Recent Patents on Biotechnology, Bentham Science Publishers, USA

• 2006.9 - 2009.2     Lecturer, Functional Genomics Major, University of Science and Technology (UST), Daejeon, Korea

• 2004.7 - 2008.12   Senior Research Scientist, Systems Microbiology Research Center, KRIBB2003.7 - 2004.6     Senior Research Scientist, Genome Research Center, KRIBB

• 2003   - 2006         Committee Member, Korean Society for Bioinformatics (KSBI)

• 2002.11 - 2003.6    Post. Doc National Center for Genome Informatics, KRIBB

• 1998. 3 - 2002. 2    Teaching Assistant, Department of Chemical Engineering, Korea Advanced Institute of Science and Technology (KAIST)

• ad hoc Reviewer

• Biotechnology and Bioengineering / Biotechnology Journal / Process Biochemistry

• BMC Genomics / BMC Bioinformatics / Computer Methods and Programs in Biomedicine / Gene

Educational Background

• 1992 (B.S.) Dept. of Chemical Engineering, KAIST, Taejon, Korea

• 1997 (M.S.) Dept. of Chemical Engineering, KAIST, Taejon, Korea

• 2002 (Ph.D.) Dept. of Chemical & Biomolecular Engineering, KAIST, Taejon, Korea

Experience and Academic Activity

• 1998.3 - 2002.8   Department of Chemical & Biomolecular Engineering, KAIST (Ph.D.)

(Dissertation: Development of Escherichia coli DNA microarray and global gene expression profiling in Escherichia coli. Supervor: Sang Yup Lee)

• 1996.3 - 1998.2   Department of Chemical Engineering, KAIST (M. S.)

(Dissertation: Development of fermentation strategies for the production of poly (γ-lutamic acid) and insulin-like growth factor I. Supervisor: Sang Yup Lee)

• 1992.3 - 1996.2   Department of Chemical Engineering, KAIST (B. S.)

Research Interests

• Metabolic Engineering / Synthetic Biology

• Pathogenomics / Host-Pathogen Interactio

• Bioinformatics / Omics Analysis

• Systems Microbiology

ad hoc Awards

• Award for Excellent Staff, KRIBB (2007.2.1)

• Award for Most Excellent Poster, Korean Society for Biotechnology and Bioengineering (2002.4.13)

Media Recognition

• 2007. 2. 6.          Daejonilbo (newspaper), "Scientist opening 21 century - Dr. Sung Ho Yoon"

• 2006.11.28.        MBC TV newstoday, "Development of pathogenicity island database"

• 2006.11.28.         YTN TV "Development of pathogenicity island database"

Publications

• 20) Yoon SH, Kim SK, and Kim JF "Secretory production of recombinant proteins in Escherichia coli" Recent Patents on Biotechnology (2010) (in press)

• 19) Barrick JE, Yu D-S, Yoon SH, Jeong H, Oh TK, Schneider D, Lenski RE, and Kim JF "Genome dynamics in a long-term experiment with Escherichia coli" Nature 461:1243-1247 (2009)

• 18) Jeong H, Barbe V, Lee CH, Vallenet D, Yu D-S, Choi S-H, Couloux A, Lee S-W, Yoon SH, Cattolico L, Hur C-G, Park H-S, Ségurens B, Kim SC, Oh TK, Lenski RE, Studier FW, Daegelen P, and Kim JF "Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)" J. Mol. Biol. (Published online 26 September 2009)

• 17) Yoon SH, Jeong H, Kwon S-K, and Kim JF "Chapter 1. Genomics, biological features, and biotechnological applications of Escherichia coli B: "Is B for better?!"", pp. 1-17, In: Systems Biology and Biotechnology of E. coli, Lee SY (ed.) Springer, Berlin, Germany (2009. 4)

• 16) Kim JF, Jeong H, Yu DS, Choi S-H, Hur C-G, Park M-S, Yoon SH, Kim D-W, Ji GE, Park H-S, and Oh TK "Genome sequence of the probiotic bacterium Bifidobacterium animalis subsp. lactis AD011" J. Bacteriol. 191:678-679 (2009)

• 15) Jeong H, Yoon SH, Yu D-S, Oh TK, and Kim JF "Recent progress in microbial genome projects of Korea" Biotechnol. J. 3:601-611 (2008)

• 14) Nam D, Yoon SH, and Kim JF "Ensemble learning of genetic networks from time-series expression data" Bioinformatics 23:3225-3231 (2007)

• 13) Yoon SH, Park YK, Lee S, Choi D, Oh TK, Hur C-G, and Kim JF "Towards pathogenomics: a web-based resource for pathogenicity islands" Nucleic Acids Res. 35:D395-D400 (2007)

• 12) Hur C-G, Kim S, Kim CH, Yoon SH, In YH, Kim C, and Cho HG "FASIM: Fragments assembly simulation using biased-sampling model and assembly simulation for microbial genome shotgun sequencing" J. Microbiol.Biotechnol. 16:683-688 (2006)

• 11) Jiang H, Shang L, Yoon SH, Lee SY, and Yu Z "Optimal production of poly-¥￧lutamic acid by metabolically engineered Escherichia coli" Biotechnol. Lett. 28:1241-1246 (2006)

• 10) Jeong H, Yoon SH, Lee HK, Oh TK, and Kim JF "Lessons from the sea: genome sequence of an algicidal marine bacterium Hahella chejuensis (적조 살상 해양 미생물 Hahella chejuensis의 유전체 구조)" Kor. J. Microbiol. Biotechnol. 34:1-6 (2006)

• 9) Jeong H, Yim JH, Lee C, Choi SH, Park YK, Yoon SH, Hur CG, Kang HY, Kim D, Lee HH, Park KH, Park SH, Park HS, Lee HK, Oh TK, and Kim JF "Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent", Nucleic Acids Res. 33:7066-7073 (2005)

• 8) Yoon SH, Hur C-G, Kang HY, Kim YH, Oh TK, and Kim JF "A computational approach for identifying pathogenicity islands in prokaryotic genomes" BMC Bioinformatics 6:184 (2005)

• 7) Yoon SH, Han MJ, Lee SY, Jeong KJ, and Yoo JS "Combined transcriptome and proteome analysis of Escherichia coli during the high cell density culture", Biotechnol. Bioeng. 81:753-767 (2003)

• 6) Kim JS, Yoon SH, and Lee SY "Development of database systems by integrating heterogeneous metabolic databases", Genome Informatics 13:486-487 (2002)

• 5) Heo WJ, Yoon SH, and Lee SY "Global gene expression profiling of E. coli and its mutant unable to produce acetate", Genome Informatics 13: 270-271 (2002)

• 4) Yoon SH and Lee SY "Comparison of transcript levels by DNA microarray and metabolic flux based on flux analysis for the production of poly-γ-glutamic acid in recombinant Escherichia coli", Genome Informatics 13:587-588 (2002)

• 3) Chung BH, Choi YJ, Yoon SH, Lee SY, and Lee YI "Process development for production of recombinant human insulin-like growth factor-I in Escherichia coli", J. Ind. Microbiol. Biotechnol. 24:94-99 (2000)

• 2) Yoon SH, Do JH, Lee SY, and Chang HN "Production of poly-γ-glutamic acid by fed-batch culture of Bacillus licheniformis" Biotechnol. Lett. 22:585-588 (2000)

• 1) Yoon SH, Choi J, and Lee SY "Development of DNA chip microarrayer" J. Microbiol.Biotechnol., 10:21-26 (2000) (The result was presented at Nature Genetics, Microarray Meeting (1999)

Patents

• 3) Jeong H, Han JH, Yoon SH, Ryu C-M, Kim JF, Oh TK, Jung MH, Yoon SP "Method for Selecting Alcohol Resistant Variants of Microorganism Using Mutagenesis by Proton Beam Irradiation" Korean Patent: appl. 10-2009-0080393 (2009.08.28)

정해영 한지희 윤성호 류충민 김지현 오태광 정명환 윤상필 “양성자 빔 조사에 의한 돌연변이 유도방법을 이용한 알코올 내성 미생물 변이체의 선별 방법” 특허출원번호10-2009-0080393 (2009.08.28)

• 2) Hong JW, Kim JF, Oh TK, and Yoon SH "A fluidic array system for biological, chemical, and biochemical assessments" PCT/US08/59777 (PCT/US08/59777) (filed on April 9, 2008)

• 1) Lee SY and Yoon SH "Methods for producing high concentration of poly (gamma-glutamic acid) by fed-batch culture of Bacillus licheniformis" Korean Patent: appl. 97-67605 (12. 10. 1997), registered 250627 (1. 5. 2000)

이상엽, 윤성호 "바실러스 리케니포니스에 의한 폴리글루탐산(g-PGA)의 고효율 생산방법" 특허출원번호 10-1997-0067605 (1997. 12. 10.), 등록번호 1002506270000 (2000. 01. 05)

Program Registration

• 2) 김지현, 윤성호, 허철구, 오태광. "미생물 유전체 내에서 외래에서 유입된 병원성 유전자군인 pathogenicity island를 예측하는 방법" (등록일자: 2007년 7월 29일; 등록번호: 2007-01-129-3962)

• 1) 김지현, 윤성호, 허철구, 박영규, 오태광. "미생물 유전체 내에서 외래에서 유입된 병원성 유전자군인 pathogenicity island를 분석, 예측하는 시스템" (등록일자: 2007년 7월 29일; 등록번호: 2007-01-129-3964)

Essays

• 6) Yoon SH "Systems and Synthetic Biology" In State of the Art Report on Microbial Genomics 미생물유전체 연구동향 분석연구), Ministry of Science and Technology, Korea (2007)

• 5)Yoon SH “최신 기술동향: Biolog사의 Phenotype Microarray (PM) Technology” 미생물과 산업, 32(2): 28-29, 한국미생물학회 (2006)6)

• 4) 이승구, 김지현, 정흥채, 송재준, 류충민, 윤성호 “Development of Platform Technologies for Synthetic Biology” (바이오기반 경제구축을 위한 합성생물학 핵심기술 개발), KRIBB, Korea (2006)

• 3) 김지현, 정해영, 윤성호, 박승환 “시스템 미생물학 : 유전체 해독에서 네트워크 재설계까지”, e-생물산업, 16(3) (2003)

• 2) Kim JS, Yoon SH, and Lee SY "미생물 게놈 연구", BioZine (http://biozine.kribb.re.kr) (2001.6)

• 1) Lee SY, Yoon SH, Im GB, and Cho YK "Genomics와 DNA chip" News & Information for Chemical Engineers, 18(3): 307-311 (2000)

Invited talks

• 14) "Computational identification of pathogenicity islands in prokaryotic genomes" KOGO 2009 Winter Symposium, YongPyung Resort, Gangwon-do, Korea (2009.01.15)

• 13)  "Multi-omics-based Systems Approach Captures the Whole-cellular Features of closely related Escherichia coli B and K-12" International Symposium on Microbiology, Daejeon convention center, Daejeon, Korea (2008. 5.16)

• 12)  "Integrated Omics Analysis of Escherichia coli B and K-12" International Meeting of the Federation of Korean Microbiological Societies, Seoul KyoYuk MunHwa HoeKwan, Seoul, Korea ( 2007.10.12)

• 11)  "In silico identification of pathogenicity islands in pathogenic genomes", BIO KOREA 2007, COEX, Seoul , Korea (2007. 9.13)

• 10)  "General Concepts of Systems Biology", National Institute for Mathematical Sciences (NIMS), Daejeon, Korea (2007. 7.13)

• 9)      "PAIDB: a web-based resource for pathogenicity islands", 신변종 전염병 출현 조기탐지 기반사업 Workshop, Korea Centers for Disease Control and Prevention, Seoul, Korea (2007. 6.22)

• 8)      "Systems Microbiology" Korea Institute of Oriental Medicine, Daejeon , Korea (2007. 5.1)

• 7)   "Computational approach for identifying genomic and pathogenicity islands in prokaryotic genomes", National Institute for Mathematical Sciences (NIMS), Daejeon, Korea (2007. 2.28)

• 6)      "Microarray Experiment and Image Analysis", The Korean Society for Microbiology and Biotechnology. BEXCO Convention Hall, Pusan, Korea (2006. 6. 18)

• 5)      "Systems Microbiology" Korea Maritime University, Division of Civil and Environmental Engineering (Major of Environmental Engineering), Pusan, Korea (2006. 5. 4)

• 4)      "Genome chip analysis workshop" KRIBB Conference - Synthetic and Systems Biology of Escherichia coli, KRIBB, Daejeon, Korea (2006. 4.27)

• 3)   "A Computational Approach for Identifying PathogenicityIslands in Prokaryotic Genomes", The Korean Society for Microbiology and Biotechnology. Korea University, Seoul , Korea (2005. 7. 1)

• 2)      "Genomic Islands & Pathogenicity Islands", International Meeting of the Federation of Korean Microbiological Societies, Seoul , Korea (2005.10.21)

• 1)      "Applications of DNA microarray: transcriptome profiling", The 1st  International Symposium on Environmental Biosensors and DNA Microarrays for Environmental Biomonitoring, Kwangju Institute of Science and Technology, Korea (2002.5.20)

Research Interest

• Protein Technology

• Directed evoloution

• Cell surface display

• Secretion of recombinant proteins from microorganism

• Extracellular production of recombinant proteins

• Recombinant protein production

• High cell density culture

• Transcriptome Analysis Metabolic Engineering

• Systems Biotechnology

• Genomic Engineering

Education

• 1995 (B.S.) Dept. of Biological Engineering, Inha Univ., Incheon, Korea

• 1997 (M.S.) Dept. of Biological Sciences, KAIST, Daejeon, Korea

• 2003 (Ph.D.) Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon, Korea

Research Activity

• International Journals

• Choi, J. H., Jun, S. C., Lee, G. M., "Effect of serum concentration on retroviral vector production from the amphotropic ψCRIP murine producer cells", Bioproc. Eng. (1997) 17, 349-353

• Lee, G. M., Choi, J. H. Jun, S. C. Passon, B. O., "Temperature significantly affects retroviral vector production and deactivation rates, and thereby determines retroviral titers", Bioproc. Eng. (1998) 19, 343-349

• Choi, J.H., Jeong, K.J., Kim, S.C., and Lee, S.Y., "Efficient secretory production of alkaline phosphatase by high cell density culture of recombinant Escherichia coli using the Bacillus sp. Endoxylanase signal sequence", Appl. Microbiol. Biotechnol. 53:640-645 (2000)

• Lee, S. Y., Choi, J.H., and Xu, Z., "Microbial cell surface display", Trends Biotechnol. (2003) 12(1): 45-52

• Choi, J.H., Lee, S.J., Lee, S.J., and Lee, S.Y., "Enhanced production of IGF-I fusion protein in Escherichia coli by the coexpression of the down -regulated genes identified by transcriptome profiling", Appl. Environ. Microbiol., 69(8): 4737-4742 (2003).

• Choi, J.H. and Lee, S.Y., "Secretory and extracellular producion of recombinant proteins using Escherichia coli", Appl. Microbiol. Biotechnol., 64 : 625-635 (2004).

• Jeong, K.J., Choi, J.H., Yoo, W.M., Keum, K.C., Yoo, N.C., Lee, S.Y. and Sung, M.-H. "Constitutive Production of Human Leptin by Fed-batch Culture of Recombinant rpoS- Escherichia coli", Protein Exp. Purif. 36 : 150-156 (2004).

• Lee, S.H., Choi, J.H., Park, S.H., Choi, J.-I., and Lee, S.Y., "Enantioselective resolution of racemic compounds by cell surface displayed lipase", Enzyme Microbial Technol., 35: 429-436. (2004).  

• Choi, J.H., Choi, J.I. and Lee, S.Y., " Display of Proteins on the Surface of Escherichia coli by C-Terminal Deletion Fusion to the Salmonella typhimurium OmpC” J. Microbiol. Biotechnol. 15(1):141-146  (2005)

• Lee, S.H., Choi, J.-I., Han, M.-J., Choi, J.H., and Lee, S.Y. "Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its applications to enantioselective resolution in organic solvent", Biotechnol. Bioeng. 90(2):223-230 (2005).

• Lee, S.Y., Choi, J.H. and Lee, S.J., "Engineering therapeutic protein production in microbial host cells", in Therapeutic Proteins: Methods and Protocols (ed. Smales C.M. and James D.C.), Humana Press, Totowa, NJ., USA., 308: 31-41 (2005).

• Choi, J.H. and Lee, S.Y., "Production of recombinant proteins by high cell density culture of Escherichia coli", Chem. Eng. Sci., 66: 876-885. (2006).

• International Proceedings

• 1. Lee, S.Y., and Choi, J.H., "Secretory Production of recombinant proteins using an artificial signal sequence. Biochemical Engineering XI, Park city", Utah, July (1999)

• 2. Jeong, K.J., Choi, J.H., Lee, S.Y., "Secretory production of proteins in E.coli : focus on excretion to the culture medium", Biochemical Engineering XII, Sonoma, California, USA, June (2001)

• 3. Jeong, K.J., Choi, J.H., Lee, S.Y., "Secretory production of proteins in E.coli : focus on excretion to the culture medium", Biochemical Engineering XII, Sonoma, California, USA, June (2001)

• 4. Choi, J.H., Lee, S.J. and Lee, S.Y., "Transcriptome analysis of Escherichia coli during production of IGF-1 by fed-batch fermentation", Proceeding of GIM2002 9th international Symposium on the Genetics of Industrial Microorganisms, p.110, Gyeongju, Korea, July 1-5 (2002)

• Patents

• 1. 국내 특허 출원 9 건

• 2. 국내 특허 등록 5 건

• 3. 국제 특허 출원 6 건

• 4. 국제 특허 등록 1 건

• Korean Journals

• 1. Choi J. H., Chun, S. C and Lee G. M., "Efficient productioin of retroviral vector for gene therapy, Biochemical Engineering, 10, 10-17 (1996)

• 2. Choi, J.H. and Lee, S.Y., "Secretion of proteins in E.coli", Recent Advances in Bioprocess Engineering 5: 81-94 (1997).

• International Conferences

• 1. Lee, S.Y. and Choi, J.H., "Secretory Production of recombinant proteins using an artificial signal sequence", Biochemical Engineering XI, Park city, Utah, July (1999)

• 2. Jeong, K.J., Choi, J.H., and Lee, S.Y., "Secretory production of proteins in E.coli: focus on excretion to the culture medium", Biochemical Engineering XII, Sonoma, California, USA, June (2001)

• 3. Choi, J.H., Lee, S.J. and Lee, S.Y., "Transcriptome analysis of Escherichia coli during production of IGF-1 by fed-batch fermentation", GIM2002 9th international Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea, July 1-5 (2002)

• 4. Choi J.H., Lee, S. J. and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of IGF-1 by transcriptome analysis", Metabolic Engineering IV applied Systems Biology, Castelvecchio Pascoli, Italy, October 6-11 (2002)

• 5. Lee, S. H., Choi J.H., and Lee, S.Y., "Resolution of racemic compounds by genetically immobilized lipase", Metabolic Engineering IV applied Systems Biology, Castelvecchio Pascoli, Italy, October 6-11(2002)

• 6. Choi J.H., Park, J.P., Park, S.J.and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of polyhydroxyalkanoates consisting of short-chain-lenth and medium-chain-length monomers", Metabolic Engineering IV applied Systems Biology, Castelvecchio Pascoli, Italy, October 6-11 (2002)

• 7. Choi, J.H., Lee, S.H., and Lee, S.Y., "Display of polyhistidine and lipase on the surface of Escherichia coli by using the salmonella ompC as an anchoring motif and its application to chemoenzymatic synthesis", Biochemical Engineering (XIII) 2003, Boulder, Colorado, USA July 17-23 (2003)

• 8. Lee, S.H, Choi, J.H., and Lee, S.Y., "Dynamic kinetic resolution using cell surface displayed biocatalysts", Biochemical Engineering (XIII) 2003, Boulder, Colorado, USA July 17-23 (2003)

• 9. Choi, J.H, Bang, H.O., and Lee, S.Y., "Display of proteins on the surface of Escherichia coli by C-terminal deletion fusion method", APBioChEC '03, Carlton Crest Hotel, Brisbane, Australia, 30 Nov - 4 December (2003)

• 10. Choi, J.H., Heo, W.J., Lee, S.J., and Lee, S.Y., "Global expression profiling of acetyl phosphate-free mutant in Escherichia coli", APBioChEC '03, Carlton Crest Hotel, Brisbane, Australia, 30 Nov - 4 December (2003)

• 11. Choi, J.H., Lee, S.H., and Lee, S.Y., "Production of chiral compounds by cell surface displayed lipase", APBioChEC '03, Carlton Crest Hotel, Brisbane, Australia, 30 Nov - 4 December (2003)

• 12. Choi, J.H., Han, M.J., Jeong, K.J., and Lee, S.Y., "Using proteomics to engineer improved cell growth and increased production of heterologous proteins in Escherichia coli", APBioChEC '03, Carlton Crest Hotel, Brisbane, Australia, 30 Nov - 4 December (2003)

• 13. Lee, S.Y., Lee, S.J., Yoo, S.M., and Choi, J.H., "Protein arrays for protein-protein interactions analysis", APBioChEC '03, Carlton Crest Hotel, Brisbane, Australia, 30 Nov - 4 December (2003)

• 14. Lee, S.Y., Bang, H.O., Lee, S.J., and Choi, J.H., "Lycopene overproduction in metabolically engineered Escherichia coli by high cell density culture", APBioChEC '03, Carlton Crest Hotel, Brisbane, Australia, 30 Nov - 4 December (2003)

• 15. Park, J.P., Park, T.J., Lee, S.J., Choi, J.H., and Lee, S.Y., "Self-assembly of functional bacterial surface protein around carbon nanotube and its use in nanostructure fabrication", 227th ACS National Meeting, Anaheim, CA, March 28-April 1 (2004)

• 16. Park, J.H., Kim,B.H., Choi, J.H., Lee, D.Y. and  Lee, S.Y., "Identification of growth-associated regulatory circuit of FadR by transcriptome profiling", The 12th International Conference on Intelligent Systems for Molecular Biology (ISMB 2004), Glasgow, UK, July 31-August 4 (2004)

• 17. Choi, J.H., Woo, H.M., and Lee, S.Y., ”Extracellular production of recombinant proteins in Escherichia coli”, Metabolic Engineering V: Genome to Product, Lake Tohoe, California, USA Sept. 19-23(2004)

• 18. Choi, J.H., "Systems biotechnological strategies for the enhanced production of recombinant proteins”, 3rd Recombinant Protein Production Meeting, Tavira, Portugal, Nov 11-14 (2004).

• 19. Lee, S.Y., Park, J.H., Kim, B.H, and Choi, J.H., "Identification of growth-associated expression pattern of FadR-responsive genes by transcriptome profiling”, 3rd Recombinant Protein Production Meeting, Tavira, Portugal, Nov 11-14 (2004).

• 20. Choi, J.H., Jeong, K.J., Lee, S.Y, and Sung, M.-H., "Constitutive production of recombinant protein by fed-batch culture of rpoS- Escherichia coli”, 3rd Recombinant Protein Production Meeting, Tavira, Portugal, Nov 11-14 (2004).

• 21. Choi, J.H., Choi, H.S. and Lee, S.Y., "Efficient production of small therapeutic peptides in recombinant Escherichia coli”, 3rd Recombinant Protein Production Meeting, Tavira, Portugal, Nov 11-14 (2004).

• 22. Choi, J.H., Lee, S.Y., “Efficient Production of Small Therapeutic Peptides in Recombinant Escherichia coli”, Asia Pacific Biochemical Engineering Conference (APBioChEC'05), Lotte Jeju Hotel, Jeju Island, Korea, May 15-19, (2005)

• Korean Proceedings

• 1.  Choi, J.H. and Lee, S.Y., "Expression of Recombinant Proteins using pH-inducible Promoter System", Proceedings of BPERC International Symposium '97, 151-152 (1997).

• 2.  Choi, J.H. and Lee, S.Y., "Effect of induction condition on expression of recombinant protein using pH-inducible promotor system", Proceedings of The 10th Daejeon/Chungnam-Kyushu Symposium

• 3.  Jeong, K.J., Choi, J.H., Lee, S.Y., and Kim, S.C., "Efficient secretion of recombinant endoxylanase in Escherichia coli", Proceedings of BPERC International Symposium '98, 173-174 (1998)

• 4.  Choi, J.H. and Lee, S.Y., "Efficient secretion of protein by strong promoters in Escherichia coli", Proceedings of BPERC International Symposium '98, 175-176 (1998)

• 5.  Choi, J.H. and Lee, S.Y., "Efficient secretion of recombinant endoxylanase by strong promoters in Escherichia coli", Proceedings of Korea Institute of Biotechnology and Bioengineering Symposium, 373-374(1998)

• 6.  Choi, J.H., Jeong, K.J., and Lee, S.Y., "Construction of secretion vector containing endoxylanase signal sequence from Bacillus subtilis and secretory production of alkaline phosphatase in Escherichia coli", Proceedings of Korea Institute of Biotechnology and Bioengineering Symposium, 509-512 (1999)

• 7.  Choi, J.H. and Lee, S.Y., "Secretory production of recombinant proteins by high cell density culture of recombinant Escherichia coli using the the Bacillus sp. endoxylanase signal sequence", Proceedings of The 12th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, 129-130 (1999)

• 8.  Choi, J.H. and Lee, S.Y., "Secretory production of recombinant proteins by high cell density culture of Escherichia coli", Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 329-330 (1999)

• 9.  Choi, J.H., Xu, Z., and Lee, S.Y., "Surface Display of Poly(His)n on the Escherichia coli using OmpC as Surface anchoring Motif", Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 523-526 (2000)

• 10. Han, M.J., Choi, J.H., Jeong, K.J., Yoo, J.S., and Lee, S.Y., "Proteome Analysis of Overproduction of Recombinant Protein in Escherichia coli by Fed-Batch Fermentations", Proceedings of Korean Society for Biotechnology and Bioengineering Symposium,750-753, November (2001)

• 11. Han, M.J., Choi, J.H., Jeong, K.J., Yoo, J.S., and Lee, S.Y., "Proteome Analysis of Overproduction of Human Leptin in Escherichia coli by Fed-Batch Fermentations", Theories and Applications of Chemical Engineering 7(2): 4075-4078 (2001).

• 12. Choi, J.H., Lee, S.H., Park, S.H., and Lee, S.Y., "Chiral Resolution by Lipase on the Escherichia coli Cell Surface", Theories and Applications of Chemical Engineering, 7(2): 4027-4030 (2001).

• 13. Kang, Y.J., Choi, J.H., Lee, S.J., and Lee, S.Y., “High-level expression of silk protein in Escherichia coli”, Proceedings of The 17th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, 37 (2004)

• Korean Conferences

• 1.  Choi, J.H. and Lee, S.Y., "Secretion of proteins in E. coli", Recent Advances in Bioprocess Engineering, 5: 81-94 (1997).

• 2.  Choi, J.H. and Lee, S.Y., "Expression of recombinant proteins using pH-inducible promoter system", Annual Symposium of KIBB, Taegu Univ., Kyungsan, October (1997)

• 3.  Choi, J.H. and Lee, S.Y., "Effect of induction condition on expression of recombinant protein using pH-inducible promotor system", The 10th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, KAIST, Teajon, November (1997)

• 4.  Jeong, K.J., Choi, J.H., Lee, S.Y., and Kim, S.C., "Efficient secretion of recombinant endoxylanase in Escherichia coli", Annual Symposium of BPERC '98, KAIST, Daejeon, Korea, September (1998)

• 5.  Choi, J.H. and Lee, S.Y., "Efficient secretion of protein by strong promoters in Escherichia coli", Annual Symposium of BPERC '98, KAIST, Daejeon, Korea, September (1998)

• 6.  Choi, J.H. and Lee, S.Y., "Efficient Secretion of Recombinant Endoxylanase by Strong Promoters in Escherichia coli", Proceedings of Korea Institute of Biotechnology and Bioengineering Symposium, 373-374(1998)

• 7.  Choi, J.H., Jeong, K.J., and Lee, S.Y., "Construction of secretion Vector containing Endoxylanase Signal Sequence from Bacillus subtilis and Secretory Production of Alkaline Phosphatase in Escherichia coli", Annual Symposium of Korea Institute of Biotechnology and Bioengineering (KIBB) Chunnam Univ., Kwangju (1999)

• 8.  Choi, J.H. and Lee, S.Y., "Secretory Production of Recombinant proteins by High Cell Density Culture of Recombinant Escherichia coli using the Bacillus sp. Endoxylanase Signal Sequence", The 12th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, Kongju Univ., Kongju (1999)

• 9.  Choi, J.H. and Lee, S.Y., "Secretory production of recombinant proteins by high cell density culture of Escherichia coli", Annual Symposium of Korean Society for Biotechnology and Bioengineering, Kangwon University, Chuncheon (1999)

• 10. Choi, J.H., Xu, Z., and Lee, S.Y., "Surface Display of Poly (His)n on the Escherichia coli using OmpC as Surface anchoring Motif", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, KAIST, April (2000)

• 11. Choi, J.H., Xu, Z., and Lee, S.Y., "Physiological Study on Recombinant Escherichia coli Displaying Poly-His Peptides", Annual Meeting of The Korean Society for Applied Microbiology, Yonsei Univ., Seoul (2000).

• 12. Choi, J.H. and Lee, S.Y., "Physiological study on bacteria cell surface display systems using OmpC", Annual Meeting of The Korean Society for Applied Microbiology, Cheon-buk Univ., October (2000)

• 13. Choi, J.H. and Lee, S.Y., "Surface display of recombinant protein on the Escherichia coli using OmpC", Annual Meeting of Korean Society for Applied Microbiology, Chonan Sangnok Resort, June (2001)

• 14. Han, M.J., Choi, J.H., Jeong, K.J., Yoo, J.S., and Lee, S.Y., "Proteome Analysis of Overproducing Human Leptin System", The 14th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, Chungnam Univ., Daejeon, Korea (2001)

• 15. Han, M.J., Choi, J.H., Jeong, K.J., Yoo, J.S., and Lee, S.Y., "Proteome Analysis of Overproduction of Recombinant Protein in Escherichia coli by Fed-Batch Fermentations", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, November (2001)

• 16. Han, M.J., Choi, J.H., Jeong, K.J., Yoo, J.S., and Lee, S.Y., "Proteome Analysis of Overproduction of Human Leptin in Escherichia coli by Fed-Batch Fermentations", KIChE Meeting, Daejeon, Korea (2001)

• 17. Choi, J.H., Lee, S.H., Park, S.H., and Lee, S.Y., "Chiral Resolution by Lipase on the Escherichia coli Cell Surface", KIChE Meeting, Daejeon, Korea (2001)

• 18. Choi, J.H., Kang, K.H., and Lee, S.Y., "Efficient production of S-layer protein using Geobacillus sp.", Annual Meeting of Korean society for Biotechnology and Bioengineering Symposium, Chungju, October 12(2002)

• 19. Park, S. J, Choi, J.H., and Lee, S.Y., "Production of Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Fed-Batch Culture of Recombinant Escherichia coli in a Pilot Scale Fermentor", Korean Society for Biology and Biotechnology Meeting, Konkuk Univ., October 26 (2002)

• 20. Choi, J.H., Lee, S.J., and Lee, S.Y., "Increasing of Productivity of Recombinant Proteins by Transcriptome Analysis in Escherichia coli", Korean Society for Biology and Biotechnology Meeting, Konkuk Univ. October 26(2002)

• 21. Choi, J. H. and Lee, S.Y., "Cell Surface Display on Escherichia coli", Annual meeting of Korean Society for Biotechnology and Bioengineering Symposium, Busan, Korea, April 11-12(2003)

• 22. Lee, S.H., Choi, J.H., and Lee, S.Y., "Production of chiral compounds by cell surface displayed lipase", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Busan, Korea, April 11-12(2003)

• 23. Lee, S.H., Choi, J.H., and Lee, S.Y., "Kinetic resolution of beta-lactams by cell surface displayed Lipase", Annual Spring Meeting of KIChE, Soonchun, April 25-26(2003)

• 24. Kang, K.H., Lee, S.Y., and Choi, J.H., "Efficient Production of S-layer Protein Using Geobacillus sp. And its Characterization", Annual Meeting of Korean Society for Microbiology and Biotechnology Symposium, Muju, Korea, June 24-26(2003)

• 25. Choi, J.H., and Lee, S.Y., "Enhanced Production of Recombinant Protein in Escherichia coli by the Coexpression of the Down-Regulated Genes Identified by Transcriptome Profiling", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, Korea, October 22-24(2003)

• 26. Choi, J.H., Jeong, K.J., and Lee, S.Y., "Enhanced production of recombinant proteins in Escherichia coli by filamentation suppression", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, Korea, October22-24(2003)

• 27. Choi, J.H. and Lee, S.Y., "Display of Polyhistidine and Lipase on the Surface of Escherichia coli by using the Salmonella OmpC as an Anchoring Motif and its Application to bioconversion", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, Korea, October 22-24(2003)

• 28. Choi, J.H., and Lee, S.Y., "Transcriptome profiling of IGF-I fusion protein production in Escherichia coli", KSBI 2003, KAIST, Daejeon, October 31-November 1(2003)

• 29. Song, A.J., Park, S.J., Choi, J.H., and Lee, S.Y., "Biosynthesis and characterization of polyhydroxyalkanoates produced by newly screened strain Pseudomonas sp. MBEL 6-19", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Daegu, Korea, April 16-17(2004)

• 30. Choi, J.H., Jeong, K.J., and Lee, S.Y., "Constitutive production of recombinant proteins by Fed-batch culture of recombinant Escherichia coli", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Daegu, Korea, April 16-17(2004)

• 31. Lee, S.J., Park, S.J., Park, T.J., Choi, J.H., and Lee, S.Y., "Self-assembly of functional bacterial surface protein and its use in nanobiotechnology", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Daegu, Korea, April 16-17(2004)

• 32. Park, J.H., Kim, B.H., Choi, J.H., and Lee, S.Y., "Growth-associated transcriptome profiling of Escherichia coli fadR mutant", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Daegu, Korea, April 16-17(2004)

• 33. Park, J.H., Kim, B.H., Choi, J.H., and Lee, S.Y., "Transcriptome profiling of Escherichia coli mutant fadR" Annual Spring Meeting of KIChE, Kongju University, April 23-24(2004)

• 34. Choi, J.H., Jeong, K.J., Lee, S.Y., and Sung, M.H., “High Level Production of Human Leptin using Constitutive Promoter in rpoS Escherichia coli”, Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Cheongju, Korea, October 13-15 (2004)

• 35. Park, J.P., Lee, S.J., Choi, J.H., Lee, S.Y., "Self-Assembly of Functional Bacterial Surface Protein around Carbon Nanotubes and Its Application", ISNM, Daejeon, November 3-5 (2004)

• 36. Kang, Y.J., Choi, J.H., Lee, S.J., and Lee, S.Y., “High-level expression of silk protein in Escherichia coli”, The 17th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering,  December 4-5 (2004)

• 37. Choi, J.H., Lee, S.Y., "System approach towards efficient production of therapeutic proteins by recombinant Escherichia coli", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, April 15-16 (2005)

• 38. Kang, Y.J., Choi, J.H., Lee, S.J. and Lee,S.Y., "High-level expression of silk protein in Escherichia coli", Annual Spring Meeting of KIChE, April 22-23 (2005)

• 39. Choi, J.H., Lee, S.J. and Lee,S.Y., "Peptide chips immobilized with protein layer for protein kinase assay", Annual Spring Meeting of KIChE, April 22-23 (2005)

• 40. Choi, J.H., Lee, S.H. and Lee, S.Y., Display of Lipase on the Ell Surface of Escherichia coli OprF", Annual Spring Meeting of KIChE, April 22-23 (2005)

• 41. Park, J.H., Lee, J.W., Kim, B.H., Choi, J.H., and Lee, S.Y., “Global analyses of transcriptomes and proteomes of Escherichia coli fadR mutant”, The 62th Korean Society for Biochemistry and Molecular Biology(KSBMB) annual meeting, Seoul, Korea, May 19-20 (2005)

• 42. Kang, Y.J., Choi, J.H., Lee, S.J., and Lee, S.Y., “High level production of recombinant silk protein in Escherichia coli”, The 62th Korean Society for Biochemistry and Molecular Biology(KSBMB) annual meeting, Seoul, Korea, May 19-20 (2005)

• 43. Choi, J.H, and Lee, S.Y., "System approach towards efficient production of therapeutic proteins by recombinant Escherichia coli", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, April 15-16 (2005)

• 44. Kang, Y.J., Choi, J.H., Lee, S.J. and Lee,S.Y., "High-level expression of silk protein in Escherichia coli", Annual Spring Meeting of KIChE, April 22-23 (2005)

• 45. Choi, J.H., Lee, S.J. and Lee, S.Y., "Peptide chips immobilized with protein layer for protein kinase assay", Annual Spring Meeting of KIChE, April 22-23 (2005)

• 46. Choi, J.H., Lee, S.H. and Lee, S.Y., Display of Lipase on the Ell Surface of Escherichia coli OprF", Annual Spring Meeting of KIChE, April 22-23 (2005)

• 47. Park, J.H., Lee, J.W., Kim, B.H., Choi, J.H., and Lee, S.Y., “Global analyses of transcriptomes and proteomes of Escherichia coli fadR mutant”, The 62th Korean Society for Biochemistry and Molecular Biology(KSBMB) annual meeting, Seoul, Korea, May 19-20 (2005)

• 48. Kang, Y.J., Choi, J.H., Lee, S.J., and Lee, S.Y., “High level production of recombinant silk protein in Escherichia coli”, The 62th Korean Society for Biochemistry and Molecular Biology(KSBMB) annual meeting, Seoul, Korea, May 19-20 (2005)

• 49. Choi, J.H., Lee, S.Y., “Extracellular Production of Recombinant Proteins in Escherichia coli”, Annual Fall Meeting of KIChE, October 21-22 (2005)

• 50. Xiao, X.X., Qian, Z.G., Choi, J.H., Yoo, N.C., Lee, S.Y., “Highly Efficient Extracellular Production of Human β-Endorphin in Recombinant Escherichia coli by Using Outer Membrane Protein F as a Fusion Partner”, International Symposium and annual meeting of KSBB, October 27-29 (2005)

Research Interest

• Metabolic engineering

• Recombinant protein production

Education

• B.S., Dept. of Chemical Engineering, KAIST, Daejeon, Korea (1993. 3. 1.~ 1997. 2. 29.)

• M.S., Dept. of Chemical Engineering, KAIST, Daejeon, Korea (1997. 3. 1.~ 1999. 2. 28.)

• Ph.D., Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon, Korea (1999. 3. 1. ~ 2003. 8. 31.)

Honor/Awards

• IBM Basic Research Award, Korean Society for Bioinformatics (2002. 11. 16.)

Experience and Academic Activity

• International Journal

• 1. Lee, S.Y. , Chang, H.N., Um, Y.S., and Hong, S.H., "Bacteriorhodopsin production by cell recycle culture of Halobacterium halobium", Biotechnol. Lett., 20(8): 763-765 (1998).

• 2. Hong, S.H. and Lee, S.Y., "Metabolic Flux distribution in metabolically engineered Escherichia coli strain producing succinic acid", J. Microbiol. Biotechnol., 10(4) : 496-501 (2000).

• 3. Hong, S.H. and Lee, S.Y., "Metabolic flux analysis for succinic acid production by recombinant Escherichia coli with amplified malic enzyme activity", Biotechnol. Bioeng., 20;74(2):89-95(2001).

• 4. Hong,S.H. and Lee,S.Y., "Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli", Appl. Microbiol. Biotechnol., 58 : 286-290(2002).

• 5. Lee, P.C., Lee, S.Y., Hong, S.H., and Chang, H.N. "Isolation and characterization of a new succinic acid producing bacterium, Mannheimia succiniciproducens MBEL 55E, from bovine rumen", Appl. Microbiol. Biotechnol., 58 : 663-668 (2002).

• 6. Lee, S.Y., Hong S.H., and Moon S.Y. "In Silico Metabolic Pathway Analysis and Design: Succinic acid Production by Metabolically Engineered Escherichia coli as an example", Genome Informatics 2002, 13: 214-223 (2002)

• 7. Lee, P.C., Lee, S.Y, Hong, S.H., and Chang, H.N. "Biological conversion of wood hydrolysate to succinic acid by Anaerobiospirillum succiniciproducens", Biotechnol.Lett., 25: 111-114 (2003)

• 8. Hong, S.H., Park, S.J., Moon, S.Y., Park, J.P. and Lee, S.Y., "In silico prediction and validation of the importance of Entner-Doudoroff pathway in poly(3-hydroxybutyrate) production by metabolically engineered Echerichia coli", Biotech. Bioeng., 83: 854-863 (2003).

• 9. Hong, S.H., Moon, S.Y. and Lee, S.Y. "Prediction of the maximum yields of metabolites and optimal pathways for their production by metabolic flux analysis", J. Microbiol. Biotechnol., 13: 571-577 (2003).

• 10. Lee, P.C., Lee, S.Y., Hong, S.H., and Chang, H.N. "Batch and Continuous cultures of Mannheimia succiniciproducens MBEL55E for the production of succinic acid from whey and corn steep liquor", Bioproc. Biosystems Eng., in press.

• 11. Hong, S.H. and Lee, S.Y., "Evaluating evolutionary relationships of microorganisms and cellular metabolic pathways based on genome-scale metabolic pathway content", submitted.

• 12. Hong, S.H., Park, S.J. and Lee, S.Y., "Metabolic flux profiling and clustering: New strategies for identification of microbial metabolic characteristics", submitted.

• 13. Moon, S.Y., Hong, S.H., and Lee.S.Y., "Production of malic acid by the amplification of Mannheimia succiniproducens phosphoenolpyruvate carboxykinase in Escherichia coli", submitted.

• International Proceedings

• 1. Lee, S.Y. and Hong, S.H., "Engeering of E. coli central metabolic pathways for the production of succinic acid", Proceedings of Pacifichem 2000, Hawaii, pp. 30-38 (2000).

• Korean Journal

• 1. Um, Y.S., Park, J.T., Hong, S.H., Lee, S.Y. and Chang, H.N. "Production of bacteriorhodopsin by Halobacterium halobium in the internal membrane bioreactor", Kor. J. Biotechnol. Bioeng. 13(3): 268-271 (1998)

• 2. Lee, P.C., Lee, S.Y., Hong, S.H., and Chang, H.N. "Cloning and characterization of Mannheimia succiniciproducens MBEL55E phophoenol pyruvate carboxykinase (pckA) gene", Biotechnol. Bioprocess Eng. 7:95-99 (2002)

• Korean Proceedings

• 1. Hong, S.H., Park, J.T., Lee, S.Y. , and Chang, H.N. "The effects of complex nitrogen sources and initial pH on growth of Halobacterium halobium S9-P", Proceedings of The 10th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, 233-234 (1997)

• 2. Hong, S.H., Lee, S.Y. and Chang, H.N., "Production of succinic acid by recombinant Escherichia coli", Proceedings of BPERC International Symposium '98, 183-184 (1998)

• 3. Hong, S.H., Park, J.T., Lee, S.Y., and Chang, H.N., "Production of bacteriorhodopsin by Halobacterium halobium S9-P using various nitrogen sources", Proceedings of BPERC International Symposium '98, 185-186 (1998)

• 4. Hong, S.H. and Lee, S.Y., "Production of succinic acid by recombinant Escherichia coli", Proceedings of The 11th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, 155-156 (1998)

• 5. Hong, S.H., Lee, S.Y., "Production of Succinic Acid by Recombinant Escherichia coli and metabolic flux analysis", Theories and Applications of Chemical Engineering 5(1): 1053-1056 (1999).

• 6. Hong, S.H., Lee, S.Y., "Production of Succinic acid by recombinant Escherichia coli and Metabolic analysis", Proceedings of The 12th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, 127-128 (1999)

• 7. Hong, S.H., Lee, S.Y., "Succinic acid production by metabolically reoriented Escherichia coli and metabolic flux analysis", Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 329-330 (1999)

• 8. Hong, S.H., and Lee, S.Y., "Effect of fumarase on the production of succinic acid", Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 531-534 (2000, Apr.)

• 9. Hong, S.H., and Lee, S.Y., "Metabolic flux analysis for succinic acid and production by recombinant Escherichia coli with amplified malic enzyme activity", Theories and Applications of Chemical Engineering 6(1): 1253-1256 (2000).

• 10. Hong, S.H., Lee, S.Y., "Prediction of intracellular metabolites accumulation in succinic acid producing recombinant Escherichia coli by metabolic flux analysis", Theories and Applications of Chemical Engineering 7(1): 1413-1416 (2001).

• 11. Hong, S.H. and Lee, S.Y., "Identification of metabolic characteristics by metabolic flux analysis", Proceeding of Annual fall meeting of KIChE, 8 (2): 3941-3944 (2002)

• International Conferences

• 1. Lee, S.Y., Hong, S.H., "Metabolic engineering of Escherichia coli for the production of succinic acid and metabolic flux analysis", Annual Meeting of Society for Industrial Microbiology, San Diego, CA, July (2000)

• 2. Hong, S.H. and Lee, S.Y., "Metabolic engineering of E. coli for the production of succinic acid", Metabolic Engineering III, Colorado Springs, Colorado, USA, October (2000)

• 3. Lee, S.Y., Hong, S.H., "Metabolic engineering of Escherichia coli for the production of succinic acid", ISBP/BEDPS 2000, Honolulu, Hawaii, December (2000)

• 4. Hong, S.H. and Lee, S.Y., "Identification of metabolic characteristics by metabolic flux analysis", GIM2002 9th international Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea, July 1-5 (2002)

• 5. Moon, S.Y., Hong, S.H., and Lee, S.Y., "Prediction of the maximum yields of various metabolites and optimal pathways by metabolic flux analysis", GIM2002 9th international Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea, July 1-5 (2002)

• 6. Moon, S.Y., Hong, S.H., and Lee, S.Y., "Metabolic flux analysis for the succinic acid production in Escherichia coli", ChungNam-Kyushu International Symposium, Kumamoto, Japan,December,6 (2002)

• 7. Lee, S.Y., Hong, S.H., and Moon, S.Y., "In Silico Metabolic Pathway Analysis and Design: Succinic acid Production by Metabolically Engineered Escherichia coli as an example", The 13th International conference on genome informatics GIW2002 meeting, Yebisu garden place, Tokyo, Japan, December 16-18(2002)

• 8. Lee, S.Y., Hong, S.H., and Moon, S.Y., "Metabolic Engineering of Escherichia coli for the Production of Succinic Acid", Symposium on Biotechnology for Fuels and Chemicals, Breckenridge, Colorado, May 4-7 (2003)

• 9. Lee, S.Y., Hong, S.H., and Moon, S.Y., "Production of Short-Length and Medium-chain-Length Polyhyroxyalkanote Copolymer by Metabolically Engineered E. coli" , Symposium on Biotechnology for Fuels and Chemicals, Breckenridge, Colorado, May 4-7 (2003)

• 10. Lee, S.Y., Hong, S.H. , Lee, P.C., and Chang, H.N., "Production of Succinic acid by Anaerobiospirillum succiniproducens from Wood Hydrolysate" , Symposium on Biotechnology for Fuels and Chemicals, Breckenridge, Colorado, May 4-7 (2003)

• 11. Lee, S.Y., Hong, S.H., Lee, P.C., and Chang, H.N., "Batch and Continuous of Mannheimia succiniciproducens MBEL55E for the production of Succinic Acid from Whey and Corn Steep Liquor" , Symposium on Biotechnology for Fuels and Chemicals, Breckenridge, Colorado, May 4-7 (2003)

• Korean Conferences

• 1. Kim, B.K., Hong, S.H., Lee, S.Y. , Lee, Y.H., and Chung, B.H., "Production of IGF-I by recombinant Escherichia coli", Annual Symposium of KIBB, Chosun Univ., Kwangju, April (1996).

• 2. Um, Y.S., Park, J.T., Hong, S.H., Lee, S.Y. , and Chang, H.N., "Production of bacteriorhodopsin by Halobacterium halobium R1 using internal and external membrane bioreactor systems", Annual Symposium of KIBB, Taegu Univ., Kyungsan, October (1997)

• 3. Hong, S.H., Park, J.T., Lee, S.Y. , and Chang, H.N., "The effects of complex nitrogen sources and initial pH on growth of Halobacterium halobium S9-P", The 10th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, KAIST, Teajon, November (1997)

• 4. Hong, S.H., Lee, S.Y., and Chang, H.N., "Production of succinic acid by recombinant Escherichia coli", Annual Symposium of BPERC '98, KAIST, Taejon, Korea, September (1998)

• 5. Hong, S.H., Park, J.T., Lee, S.Y., and Chang, H.N., "Production of bacteriorhodopsin by Halobacterium halobium S9-P using various nitrogen sources", Annual Symposium of BPERC '98, KAIST, Taejon, Korea, September (1998)

• 6. Hong, S.H. and Lee, S.Y., "Production of succinic acid by metabolically engineered Escherichia coli", Annual Symposium of KSAM, KOFST Bldg., Seoul, October (1998)

• 7. Hong, S.H., Lee, S.Y., "Production of Succinic Acid by Recombinant Escherichia coli and metabolic flux analysis", Annual Symposium of KIChE, SeongKyunKwan Univ. Suwon (1999)

• 8. Hong, S.H., Lee, S.Y., "Production of Succinic acid by recombinant Escherichia coli and Metabolic analysis", The 12th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, Kongju Univ., Kongju (1999)

• 9. Hong, S.H., Lee, S.Y., "Succinic acid production by metabolically reoriented Escherichia coli and metabolic flux analysis", Annual Symposium of Korean Society for Biotechnology and Bioengineering, Kangwon University, Chuncheon (1999)

• 10. Hong, S.H., and Lee, S.Y., "Effect of fumarase on the production of succinic acid", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, KAIST, April (2000)

• 11. Hong, S.H., and Lee, S.Y., "Metabolic flux analysis for succinic acid and production by recombinant Escherichia coli with amplified malic enzyme activity", KIChE Meeting, Hanyang Univ., Ansan , April (2000)

• 12. Hong, S.H., Lee, S.Y., "Effect of redox potential on the production of succinic acid by metabolically engineered Escherichia coli", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, Chunan, November(2000)

• 13. Hong, S.H., Lee, S.Y., "Prediction of intracellular metabolites accumulation in succinic acid producing recombinant Escherichia coli by metabolic flux analysis", KIChE meeting, Yonsei Univ., April (2001)

• 14. Hong, S.H. and Lee, S.Y., "Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli”, The 14th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, Chungnam Univ., Taejon , Korea (2001)

• 15. Hong, S.H. and Lee S.Y., "In Silico Metabolic Pathway Analysis and Design", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Chungju, October 12(2002)

• 16. Moon, S.Y, Hong. S.H., and Lee, S.Y., "Prediction of the optimal metabolic pathways for the production of metabolites in Escherichia coli", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Chungju, October 12(2002)

• 17. Hong, S.H. and Lee S.Y., "Identification of metabolic characteristics of Mannheimia succiniciproducens MBEL55E", Annual Meeting of Korean Society for Biotechnology and Bioengineering symposium, Chungju, October 12(2002)

• 18. Hong, S.H. and Lee, S.Y., "Identification of metabolic characteristics by metabolic flux analysis", Annual Fall Meeting of KIChE, Seoul, October 24-26 (2002)

• 19. Hong, S.H., Park, S.J., Moon, S.Y., Park, J.P. and Lee, S.Y., "In Silico Prediction of the Importance of Enter-Doudoroff Pathway in Poly (3-Hydroxybutyrate) Production", Korean Society for Bicrobiology and Biotechnology Meeting, Konkuk univ., October, 26 (2002)

• 20. Hong, S. H., Lee, P.C., Chang, H.N. and Lee, S.Y., "Continuous Cultures of Mannheimia succiniciproducens MBEL55E for the Production of Succinic Acid from various carbon substrates", Korean society for Bicrobiology and Biotechnology Meeting, Konkuk Univ., October, 26 (2002)

• 21. Hong, S.H., Lee, S.Y. and Moon, S.Y., "In silico metabolic network construction and metabolic flux analysis for the production of succinic acid in Escherichia coli", Korean Society For Bioinformatics Annual Meeting, Haeundae, Korea, November,15-16 (2002)

• 22. Han, M.J., Lee, S.Y., Hong, S. H., and Park, S.J., "Role of Eda in polyhydroxyalkanoate biosynthesis deciphered from proteome analysis and metabolic flux analysis", Korean Society For Bioinformatics Annual Meeting, Haeundae, Korea, November, 15-16 (2002)

• 23. Lee, S.Y., Jeong, K.J., Yoon, S.H., Han, M.J., Choi, J.H., Park, S.J., and Hong, S.H., "Integrated analysis of the transcriptome and proteome of recombinant E. coli fermentation", 2002 Nanobio International Symposium, Haeundae, Korea, November 22-23 (2002)

• 24. Hong, S.H., Moon, S.Y.and Lee, S.Y., "“Prediction of the maximum yields of metabolites by metabolic flux analysis", Annual meeting of Korean Society for Biotechnology and Bioengineering Symposium, Busan, Korea, April 11-12(2003)

• Books/Book Editing

• 1. Lee, S.Y. and Hong, S.H., "Engineering of E. coli central metabolic pathways for the production of succinic acid", In:Advances in Biochemical Production Technology(Park, T.H. and Martin, eds.), American Chemical Society, Washington DC, USA, pp.30-38 (2002)

• 2. Lee S.Y., Hong S.H., Park S.J., van Wegen, R. and Middelberg A.P.J., "Metabolic flux analysis on the prodcution of poly(3-hydroxybutyrate)", Biopolymers Vol 3a, Ch 8, pp.249-262 ,VCH-Wiley, Weinheim, Germany (2001) 

• 3. Lee, S.Y., Park, S.H., Hong, S.H., Lee, Y. and Lee, S.H., "Fermentative Production of Building Blocks for Chemical Synthesis of Polyesters", Biopolymers Vol 3b, Ch 10, pp.265-326, VCH-Wiley, Weinheim, Germany (2001)

• Patents

• 국내 특허 등록 2건, 출원 1건

Research Interest

• Metabolic engineering

• Polyhydroxyalkanoate (PHA)

• High cell density cultivation

• Protein engineering

Education

• 1993. 3.-1997. 2.  Department of Chemical Engineering, Seoul National University, Seoul, Korea, B. S 

• 1997. 3 – 1999. 2.  Department of Chemical Engineering, KAIST, Taejon, Korea M. S  (M. S. thesis : Cloning of desulfurization genes from Gordona sp. CYKS1 and its expression in Escherichia coli (Advisor: Prof. Sang Yup Lee))

• 1999. 3 – 2003. 8.  Department of Chemical Engineering, KAIST, Taejon, Korea, Ph. D. (Dissertation title: Metabolic engineering for the production of poly(3-hydroxyalkanoate) in recombinane Escherichia coli. (Advisor: Prof. Sang Yup Lee))

Publications

• International Journal

•   1. Park SJ,Lee SY (1998) "Efficient recovery of secretory recombinant protein from protease negative mutant Escherichia coli strains", Biotechnol Techniques 12: 815-818

• 2. Park SJ,Georgiou G, Lee SY (1999) "Secretory production of recombinant protein by high cell density culture of protease negative mutant Escherichia coli strain", Biotechnol Prog 15: 164-167

• 3. Ahn WS, Park SJ, Lee SY (2000) "Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution", Appl Environ Microbiol 66:3624-3627

• 4. Park SJ, Ahn WS, Green PR, Lee SY (2001) "High level production of  poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains" Biomacromolecules 2:248-254

• 5. Park SJ, Ahn WS, Green PR, Lee SY (2001) "Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains¢¢ç", Biotechnol Bioeng 74:82-87

• 6. Ahn WS, Park SJ, Lee SY (2001) "Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli" Biotechnol Lett 23:235-240

• 7. Chen GQ, Zhang G, Park SJ, Lee, SY (2001) "Industrial scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)" Appl Microbiol Biotechnol 57: 50-55

• 8. Park SJ, Park JP, Lee SY (2002) "Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermentor", Biotechnol. Lett., 24:185-189

• 9. Park SJ, Park JP, Lee SY (2002)" Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers", FEMS Microbiol. Lett., 214:217-222.

• 10. Hong SH, Park SJ, Moon SY, Park JP, Lee SY "In silico prediction and validation of the importance of enter-doudoroff pathway in poly(3-hydroxybutyrate) production by metabolically engineered Escherichia coli" Biotechnol Bioeng, 83: 854-863

• 11. Park SJ, Park JP, Lee SY, Doi Y "Enrichment of specific monomer in medium-chain-length poly(3-hydroxyalkanoates) by amplification of fadD and fadE genes in recombinant Escherichia coli" Enzyme Microb Technol., 33 : 62-70.

• 12. Park SJ, Lee I-S, Chang YK, Lee SY "Desulfurization of dibenzothiophene by metabolically engineered Escherichia coli",  J. Microbiol. Biotechnol.,  13(4):578-583.

• 13. Park S J and Lee S Y "Identification and characterization of a new enoyl Coenzyme A hydratase involved in the biosynthesis of medium-chain-length polyhydroxyalkanoates in recombinant Escherichia coli",  J. Bacteriol., 185(18) : 5391-5397.

• 14. Park, S.J., Lee, Y., and Lee, S.Y., "Biosynthesis of (R)-3-hydroxyalkanic acid by metabolically engineered E.coli", Appl. Biochem. Biotechnol., In press

• Domestic Journal

• 1. Choi J, Lee SY, Shin K, Lee WG, Park SJ, Chang HN, Chang YK (2002) "Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli", Biotechnol Bioprocess Eng 7: 371-374

• Book chapters

• 1. Lee SY, Park SJ, "Fermentative production of SCL-PHAs", in Biopolymers, Vol. 3a(Steinbuchel A Ed), Wiley-VCH, 263-290.

• 2. Lee SY, Park SJ, "Biosynthesis and fermentative production of SCL-MCL-PHAs", in Biopolymers, Vol. 3a (Steinbuchel A Ed), Wiley-VCH, 317-336.

• 3. Lee SY, Hong SH, Park SJ, van Wegen R, Middelberg APJ, "Metabolic flux analysis on the production of poly(3-hydroxybutyrate)" in Biopolymers, Vol. 3a (Steinbuchel A Ed), Wiley-VCH, 249-261.

• 4. Lee SY, Park SJ, Park JP, Lee Y, Lee SH (2002), "Economic aspects of biopolymer production" in Biopolymers, Vol. 10 (Steinbuchel A Ed), Wiley-VCH, in press.

• 5. Lee SY, Park SJ, Lee Y, Lee SH (2002), "Biotechnological Processes for the Production of Monomers for Subsequent Chemical Polymer Synthesis", in Biopolymers, Vol. 10 (Steinbuchel A Ed), Wiley-VCH, in press.

• Patents: 6 patents were pended.

• Domestic Proceedings

• 1. Park SJ, Lee SY "Production of heterogeneous protein by fed-batch culture of protease negative mutant Escherichia coli", Proceedings of The 10th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, 367-368 (1997)

• 2. Park SJ, Lee SY, Chang YK "Cloning of desulfurization genes from Gordona sp.", Proceedings of BPERC International Symposium '98, 177-178 (1998)

• 3. Park SJ, Lee SY "Production of secretory recombinant protein by fed-batch culture of protease negative mutant Escherichia coli strain", Proceedings of BPERC International Symposium '98, 179-180 (1998)

• 4. Park SJ, Lee SY "Development of efficient recovery methods for secretory recombinant protein from protease-negative mutant Escherichia coli strains", Proceedings of BPERC International Symposium '98, 181-182 (1998)

• 5. Park SJ, Lee SY "Production of heterologous protein by fed-batch culture of protease negative mutant Escherichia coli" Proceedings of The 11th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, 149-150 (1998)

• 6. Park SJ, Lee SY, Chang YK "Cloning of desulfurization genes from newly isolated Gordona sp. CYKS1",  Proceedings of Korea Institute of Biotechnology and Bioengineering Symposium, 293-294(1998)

• 7. Park SJ, Lee SY "Clonig of desulfurization genes from Gordona sp. CYKS1 and development of recombinant Escherichia coli for desulfurization", Theories and Applications of Chemical Engineering 5(1): 1145-1148 (1999).

• 8. Park SJ, Lee SY "Development of Recombinant Escherichia coli for Biodesulfurization", Proceedings of The 12th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, 123-124 (1999)

• 9. Park SJ, Ahn WS, Lee SY "Production of poly (3-hydroxybutyrate) by high cell density cultivation of recombinant Escherichia coli from whey", Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 223-226 (2000. Apr.)

• 10. Choi J, Park SJ, Lee SY "Production of Poly (3-hydroxybutyrate) with high molecular mass by recombinant Escherichia coli", Theories and Applications of Chemical Engineering 6(1): 1233-1236 (2000).

• 11. Lee SH, Park SJ, Lee SY, Park SH "Production of Medium-chain-length Polyhydroxyalkanoate from Newly Screened Pseudomonas sp. MBEL21",  Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 153-156 (2001, Apr.)

• 12. Park SJ, Ahn WS, Lee SY "Production of poly (3- hydroxybutyrate- co- 3- hydroxyvalerate- co- 3- hydroxyhexanoate by recombinant Escherichia coli strains)", Theories and Applications of Chemical Engineering 7(1): 1425-1428 (2001).

• 13. Park SJ, Park JP, Lee SY "Biosynthesis of medium-chain-length poly(3-hydroxyalkanoates) by metabolically engineered Escherichia coli strains", Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 735-738, November (2001)

• 14. Park JP, Park SJ, Lee SY "Production and characterization of ultra-high-molecular weight poly (3-hydroxybutyrate) by recombinant Escherichia coli",  Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 731-734, November (2001)

• 15. Park SJ, Park JP, Lee SY "Production of poly (3-hydroxybutyrate) by recombinant Escherichia coli strain in pilot-scale fermentor from whey", Theories and Applications of Chemical Engineering 7(2): 3943-3946 (2001)

• International Conferences

• 1. Lee SY, Ahn WS, Park SJ "Production of poly (3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli", Annual Meeting of Society for Industrial Microbiology, San Diego, CA, July (2000)

• 2. Park SJ, Ahn WS, Green PR, Lee SY "Production of poly (3-hydroxybutyrate-co-3- hydroxyhexanoate) by metabolically engineered Escherichia coli strains", The 8th Annual International Symposium on Biological Polyesters, MIT, Masachusetts, USA, September (2000)

• 3. Park SJ, Ahn WS, Lee SY "Production of poly (3-hydroxybutyrate) by fed-batch culture of recombnant Escherichia coli using highly concentrated whey solution", The 8th Annual International Symposium on Biological Polyesters, MIT, Masachusetts, USA, September (2000)

• 4. Lee SY, Ahn WS, Park SJ "High level production of poly (3-hydroxybutyrate) from whey by metabolically engineered E. coli", Metabolic Engineering III, Colorado Springs, Colorado, USA, October (2000)

• 5. Lee SH, Park SJ, Park JP, Lee SY "Biosynthesis of medium-chain-length polyhydroxyalkanoates in recombinant Escherichia Coli by engineering ¥â-oxidation pathway", GIM2002 9th international Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea, July 1-5 (2002)

• 6. Park JP, Park SJ, Lee SY "Production of medium-chain–length polyhydroxyalkanoates in metabolically engineered Escherichia Coli strains", GIM2002 9th international Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea, July 1-5 (2002)

• 7. Park SJ, Park JP, Lee SY "Biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyalkanoate) in metabolically engineered Escherichia Coli strains", GIM2002 9th international Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea, July 1-5 (2002)

• 8. Park JP, Park SJ, Lee SY "Biosynthesis and chataterization of polyhydroxalkanotes in newly screened Pseudomonas sp. MBEL21", International Symposium on Biological Polyesters,Munster, Germany, September 24(2002)

• 9. Park SJ, Park JP, Lee SY "Biosynthesis of medium-chain-length polyhydroxyalkanotates in recombinant Escherichia coli", International Symposium on Biological Polyesters, Munster, Germany, September 24(2002)

• 10. Park JP, Park SJ, Lee SY "Biosythesis and chacterization of polyhydroxyalkanotes from newly screened strain from sludge" , International Symposium on Biological Polyesters, Munster, Germany, September 22-26(2002)

• 11. Park JP, Park SJ, Lee SY "Production of (R)-(-)-3-hydroxybutyric acid by metabolically engineered Escherichia coli" , International Symposium on Biological Polyesters, Munster, Germany, September 22-26(2002)

• 12. Park JP, Park SJ, Lee SY "Amplification of glycolytic pathway increases the yield of poly(3-hydroxbutyrate) in Esxherichia coli", International Symposium Biological Polyesters, Munster, Germany, September 22-26(2002)

• 13. Choi JH, Park JP, Park SJ, Lee SY "Metabolic engineering of Escherichia coli for the production of polyhydroxyalkanoates consisting of short-chain–length and medium-chain-length monomers", Metabolic Engneering IV applied Systems Biology, Castelvecchio Pascoli, Italy, October 6-11 (2002)

• 14. Lee SH, Park JP, Park SJ, Lee SY "Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates" Metabolic Engneering IV applied Systems Biology, Castelvecchio Pascoli, Italy, October 6-11(2002)

• Domestic Conferences

• 1. Park SJ, Lee SY "Production of heterogeneous protein by fed-batch culture of protease negative mutant Escherichia coli", The 10th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, KAIST, Taejon, November (1997)

• 2. Park SJ, Lee SY, Chang YK "Cloning of desulfurization genes from Gordona sp.", Annual Symposium of BPERC '98, KAIST, Daejeon, Korea, September (1998)

• 3. Park SJ, Lee SY "Production of secretory recombinant protein by fed-batch culture of protease negative mutant Escherichia coli strain", Annual Symposium of BPERC '98, KAIST, Daejeon, Korea, September (1998)

• 4. Park SJ, Lee SY "Development of efficient recovery methods for secretory recombinant protein from protease-negative mutant Escherichia coli strains", Annual Symposium of BPERC '98, KAIST, Daejeon, Korea, September (1998)

• 5. Park SJ, Lee SY, Chang YK "Cloning of desulfurization genes from newly isolated Gordona sp. CYKS1" Annual Symposium of KIBB, Hanyang Univ., Ansan, October (1998)

• 6. Park SJ, Jeong KJ, Lee HS, Lee SY, Chang YK "Development of recombinant bacteria for the degradation of dibenzothiophene" Annual Symposium of KIChE, Chosun Univ., Kwangju, October (1998)

• 7. Park SJ, Lee SY "Clonig of desulfurization genes from Gordona sp. CYKS1 and development of recombinant Escherichia coli for desulfurization", Annual Symposium of KIChE, SeongKyunKwan Univ. Suwon (1999)

• 8. Park SJ, Lee SY "Development of Recombinant Escherichia coli for Biodesulfurization", The 12th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, Kongju Univ., Kongju (1999)

• 9. Park SJ, Ahn WS, Lee SY "Production of poly (3-hydroxybutyrate) by high cell density cultivation of recombinant Escherichia coli from whey", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, KAIST, April (2000)

• 10. Park SJ, Ahn WS, Green PR, Lee SY "Production of poly (3-hydroxybutyrate-co-3- hydroxyhexanoate) by high cell density cultivation of recomninant Escherichia coli", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, Chunan, November(2000)

• 11. Lee SH, Park SJ, Lee SY, Park SH " Production of Medium-chain-length Polyhydroxyalkanoate from Newly Screened Pseudomonas sp. MBEL21", Annual meeting of Korean society for Biotechnology and Bioengineering, Konkuk Univ., Seoul, April (2001)

• 12. Park SJ, Ahn WS, Lee SY "Production of poly (3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate by recombinant Escherichia coli strains), KIChE meeting, Yonsei Univ., April (2001)

• 13. Lee SY, Park SJ, Lee Y "Metabolic Engineering of Escherichia coli for the Production of Novel PHA and Chiral Compounds", Annual Meeting of Korean Society for Applied Microbiology, Chonan Sangnok Resort, June (2001)

• 14. Park SJ, Lee SY "Biosynthesis of medium-chain-length poly (3-hydroxyalkanoates) by metabolically engineered Escherichia coli strains", The 14th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, Chungnam Univ., Daejeon, Korea (2001)

• 15. Park JP, Park SJ, Lee SY "Production and characterization of ultra-high-molecular weight poly (3-hydroxybutyrate) by recombinant Escherichia coli", The 14th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, Chungnam Univ., Daejeon, Korea (2001)

• 16. Park SJ, Park JP, Lee SY "Biosynthesis of medium-chain-length poly (3-hydroxyalkanoates) by metabolically engineered Escherichia coli strains", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, Seoul, November (2001)

• 17. Park JP, Park SJ, Lee SY "Production and characterization of ultra-high-molecular weight poly(3-hydroxybutyrate) by recombinant Escherichia coli", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, Seoul, November(2001)

• 18. Park SJ, Park JP, Lee SY "Production of poly (3-hydroxybutyrate) by recombinant Escherichia coli strain in pilot-scale fermentor from whey", KIChE meeting, Daejeon, Korea (2001)

• 19. Park JP, Park SJ, Lee SY "Molecular cloning of (R)-specific enoyl-CoA hydratase genes from Pseudomonas sp. MBEL21 and their use for polyhydroxyalkanoate synthesis", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium,chungju ,October 12(2002)

• 20. Park SJ, Park JP, Lee SY "Evaluation of the roles of 3-ketoacyl-ACP reductases in the polyhydroxyalkanoates in recombinant Escherichia coli", Annual meeting of Korean society for Biotechnology and Bioengineering Symposium, Chungju, October 12(2002)

• 21. Hong SH, Park SJ, Moon SY, Park JP, Lee SY "In SilicoPrediction of the Importance of Enter-Doudoroff Pathway in Poly (3-Hydroxybutyrate) Production", Korean society for Bicrobiology and Biotechnology meeting, Konkuk univ. October, 26 (2002)

• 22. Park SJ, Choi J, Lee SY "Production of Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Fed-Batch Culture of Recombinant Escherichia coli in a Pilot Scale Fermentor", Korean society for Bicrobiology and Biotechnology meeting, Konkuk univ. October, 26 (2002)

• 23. Park JP, Park SJ, Lee SY " Production of medium-chain-length polyhydroxyalkanoates by a newly isolated strain of Pseudomonas sp. MBEL21",  Korean society for Bicrobiology and Biotechnology meeting, Konkuk univ. October, 26 (2002)

• 24. Park JP, Park SJ, Lee SY "Medium-chain-length polyhydroxyalkanoate biosynthesis and accumulation in metabolic engineered recombinant E. coli"  Korean society for Bicrobiology and Biotechnology meeting, Konkuk univ. October, 26(2002)

• 25. Han MJ, Lee SY, Hong SH, Park SJ "Role of Eda in polyhydroxyalkanoate biosynthesis deciphered from proteome analysis and metabolic flux analysis", Korean Society For Bioinformatics Annual Meeting, Haeundae, Korea, November, 15-16 (2002)

• 26. Lee SY, Jeong KJ, Yoon SH, Han MJ, Choi JH, Park SJ, Hong SH "Integrated analysis of the transcriptome and proteome of recombinant E. coli fermentation", 2002 Nanobio international symposium, Haeundae, Korea, November, 22-23 (2002)

Education

• PhD., Chemical and Biomolecular Engineering, present, KAIST, Daejeon, Korea. (Thesis Title: Computational and Bioinformatic Analysis of Complex Biochemical Pathways, Thesis Supervisors: Prof. Sunwon Park & Prof. Sang Yup Lee)

• MS., Chemical Engineering, Feb. 2000, KAIST, Daejeon, Korea. (Thesis Title: Environmentally Friendly Scheduling for Primary Steelmaking Processes, Thesis Supervisor: Prof. Sunwon Park)

• BS., Chemical Engineering, Feb. 1998, Yonsei Univ., Seoul, Korea

Experience and Academic Activity

• Internship, Pacific R&D Center, Pacific Corp., July 1997.

• Visiting Research Fellow, Social and Environmental Systems Division, National Institute for Environmental Studies (NIES), Tsukuba, Japan (Jan. 2000 - Feb. 2000), supported by JISTEC

Reference: Dr. Yuichi Moriguchi (Host researcher)

Research theme: Environmental Impacts for the Change of World Resource Flows in Fuel Oil Production

Photo

• Visiting Research Fellow, Institute for Algorithms and Scientific Computing (SCAI), Fraunhofer-Gesellschaft (FhG), Sankt Augustin, Germany (Jul. 2001 - Oct. 2001), supported by Korean Ministry of Education

Reference: Prof. Ralf Zimmer (Praktische Informatik und Bioinformatik, Institut für Informatik, LMU München)

Research theme: Petri Net Representation and Simulation of Signal Transduction Network

• Visiting Research Fellow, Dept. of Chem. Eng., Kansas State University, Manhattan, United State (Jan. 2003 - Apr. 2003), supported by Korean Ministry of Education & Human Resources Development

Reference: Prof. L. T. Fan

Research theme: Synergistic Identification of Multiple Flux Distributions and Multiple Metabolic Pathways

Honor/Awards

• Oct. 1990, Bronze medal, The 4th Korean Mathematical Olympiad by Korean Mathematical Society, Test paper

• Oct. 1996, 1st Prize, The 2nd Plant Design Contest sponsored by Korean Institute of Chemical Engineers

• June 2001, Best paper award, 2001 Korean Society of Supply Chain Management Spring Meeting

• 2003, Genome Informatics Workshop (GIW) Student Fellowship Award by Japanese Society for Bioinformatics (JSBi)

Software

• MetaFluxNetTM: An integrated program for the management of metabolic reaction information and quantitative metabolic flux analysis (MFA)

Version 1.0 (program registration: 29 Mar. 2002) [Registration No.: 2002-01-12-1984]

Version 1.5 (released: 15 Dec. 2002; program registration: 6 Jan. 2003) [Registration No: 2003-01-12-27]

Version 1.6 (released: 10 May 2003)

Version 1.63 (released: 4 Aug. 2003) [Download] [More]

Version 1.7 (program registration: 19 Aug. 2003) [Registration No: 2003-01-12-4370]

Publications

•  Jin-Kwang Bok, Dong-Yup Lee and Sunwon Park, "Mixed-Integer Programming Model for Scheduling of Steelmaking Processes", J. Control, Automation and Systems Eng., Vol. 5, No. 6, pp. 714-723, 1999. [PDF-Full text in Korean]

• Heejin Lim, Dong-Yup Lee and Sunwon Park, "Environmental Analysis and Improvement of PET Production Systems Using Life Cycle Assessment", Korean Journal of LCA., Vol. 2, No. 1, pp. 53-60, 2000. [PDF-Full text in Korean]

• Hodong Seo, Dong-Yup Lee, Sunwon Park, Liang-Tseng Fan, Shahram Shafie, Botond Bertók and Ferenc Friedler, "Graph-Theoretical Identification of Pathways for Biochemical Reactions", Biotechnology Letters, Vol. 23, pp. 1551-1557, 2001. [PDF-Full text]

• Jehoon Song, Jin-Kwang Bok, Heeman Lee, Hyungjin Park, Dong-Yup Lee and Sunwon Park, "SCM의 개요와 화학공학분야에서의 연구 현황 및 화학 산업의 적용 사례", ICASE Magazine, Vol. 7, No. 4, pp. 7-18, 2001. [PDF-Full text in Korean]

• Jehoon Song, Hyungjin Park, Dong-Yup Lee and Sunwon Park, "Scheduling Model for Actual Size Refinery Processes Considering Supply Chain Management", Journal of the Korean Society of Supply Chain Management, Vol. 1, pp. 73-80, 2001. [PDF-Full text in Korean]

• Jehoon Song, Dong-Yup Lee and Sunwon Park, "공습사슬 관리(SCM)의 개요와 화학공학분야에서의 연구 현황", News & Information for Chemical Engineers, Vol. 20, No. 3, pp. 289-291, 2002. [PDF-Full text in Korean]

• Jehoon Song, Hyungjin Park, Dong-Yup Lee and Sunwon Park, "Scheduling of Actual Size Refinery Processes Considering Environmental Impacts with Multiobjective Optimization", Industrial & Engineering Chemistry Research, Vol. 41, pp. 4794-4806, 2002. [PDF-Full text]

• Dong-Yup Lee, Young-gyun Oh, Hongseok Yoon, Sang Yup Lee and Sunwon Park, "Exploring Flux Distribution Profiles for Switching Pathways Using Multiobjective Flux Balance Analysis", Genome Informatics, Vol. 13, pp. 363-364, 2002. [PDF-Full text]

• Dong-Yup Lee, Moonyong Lee, Yongho Lee and Sunwon Park, "Mp Criterion Based Multiloop PID Controllers Tuning for Desired Closed Loop Responses", Korean J. Chem. Eng., Vol. 20, pp. 8-13, 2003. [PDF-Full text]

• Dong-Yup Lee, Hongseok Yun, Sunwon Park and Sang Yup Lee, "MetaFluxNet: the Management of Metabolic Reaction Information and Quantitative Metabolic Flux Analysis", Bioinformatics, Vol. 19, pp. 2144-2146, 2003. [PDF-Full text]

• Dong-Yup Lee, Su Whan Sung, Sang Yup Lee and Sunwon Park, "A Combined Deterministic-Stochastic Approach for Pharmacokinetic Modeling", submitted for Journal publication.

• Dong-Yup Lee, L. T. Fan, Sunwon Park, Sang Yup Lee, Shahram Shafie, Botond Bertók and Ferenc Friedler, "Synergistic Identification of Multiple Flux Distributions and Multiple Metabolic Pathways", submitted for Journal publication.

• Sungdeuk Moon, Dong-Yup Lee and Sunwon Park, "Scheduling of Primary Steelmaking Processes: Part 1. Mathematical Model", in preparation to be submitted to Industrial & Engineering Chemistry Research.

• Dong-Yup Lee, Sungdeuk Moon, Paulo-Miguel Lopes and Sunwon Park, "Scheduling of Primary Steelmaking Processes: Part 2. Environmentally Friendly Scheduling", in preparation to be submitted to Industrial & Engineering Chemistry Research.

Presentations

• Jehoon Song, Seokjoon Kim, Dong-Yup Lee and Doohwan Kim, "Simulation for Separation of the MCH-Toluence System", Process S/W and H/W Symposium, pp. 157-167, 1997.

• Dong-Yup Lee, Yongho Lee and Sunwon Park, "Application of SISO-DCLR Method for MIMO Systems", Proc. '98 KIChE Fall meeting, Vol. 4, pp. 2337-2340, 1998. [PDF-Full text in Korean]

• Dong-Yup Lee, Hodong Seo, Myungwan Han and Sunwon Park, "A Study on Optimization Regions for Complex Column Alterntives", The 11th Kyushu-Chungnam Symposium on Chem. Eng., pp. 339-340, Nov. 1998. [presentation]

• Changryol Kim, Dong-Yup Lee and Sunwon Park, "Life Cycle Inventory of Petroleum Products", Proc. '98 KSLCA meeting, pp. 121, 1998.

• Dong-Yup Lee, Yongho Lee and Sunwon Park, "Multiloop PID Controller Tuning for Unstable Systems", Proc. '99 KIChE Spring meeting, Vol. 5, pp. 389-392, 1999. [PDF-Full text in Korean]

• Jin-Kwang Bok, Dong-Yup Lee and Sunwon Park, "Mixed-Integer Programming Model for Scheduling of Steelmaking Processes", Proc. '99 KIChE Spring meeting, Vol. 5, pp. 513-516, 1999.

• Dong-Yup Lee, Jin-Kwang Bok and Sunwon Park, "Mixed-Integer Programming Model for Scheduling of Steelmaking Processes Considering Environmental Impacts", The 2nd Technical Society of Steel Process Control Workshop in Pohang, pp. 52-55, 1999.

• Dong-Yup Lee, Jin-Kwang Bok and Sunwon Park, "Scheduling of Steelmaking Processes Considering Environmental Impacts", The 3rd Korea-China Joint Workshop on PSE in Taejon, pp. 127-130, 1999. [PDF-Full text]

• Dong-Yup Lee and Sunwon Park, "Approximation Technique for the Generation of the Noninferior Set in Multiobjective Optimization", Proc. '99 KIChE Fall meeting, Vol. 5, pp. 2593-2596, 1999. [PDF-Full text in Korean]

• Dong-Yup Lee, Yongho Lee, Jeongseok Lee and Sunwon Park, "Multiloop PID Controller Tuning Method for Open-Loop Unstable Systems", The 12th Chungnam-Kyushu Symposium on Chem. Eng., pp. 385-386, Oct. 1999. [PDF-Full text]

• Heejin Lim, Dong-Yup Lee and Sunwon Park, "Application of LCA to PET Process", Proc. '99 KSLCA meeting, pp. 116-122, 1999. [PDF-Full text in Korean]

• Sungdeuk Moon, Dong-Yup Lee and Sunwon Park, "Mathematical Model for Scheduling of Primary Steelmaking Processes", The 4th Korea-France Workshop on Simulation, Optimization and Control in PSE in Cheju Island, pp. 123-135, 2000.

• Sungdeuk Moon, Dong-Yup Lee and Sunwon Park, "Scheduling of Primary Steelmaking Processes: Part 1. Mathematical Modeling", Proc. '00 KIChE Spring meeting, Vol. 6, pp. 377-380, 2000.

• Dong-Yup Lee, Sungdeuk Moon and Sunwon Park, "Scheduling of Primary Steelmaking Processes: Part 2. Environmentally Friendly Scheduling", Proc. '00 KIChE Spring meeting, Vol. 6, pp. 381-384, 2000. [PDF-Full text][presentation]

• Hyungjin Park, Sungdeuk Moon, Dong-Yup Lee and Sunwon Park, "Scheduling of Primary Steelmaking Processes with an Optimal Control Approach", Proc. '00 KIChE Spring meeting, Vol. 6, pp. 413-416, 2000.

• Heejin Lim, Dong-Yup Lee and Sunwon Park, "Life Cycle Assessment for Two Different PET Production Systems", Proc. '00 KIChE Spring meeting, Vol. 6, pp. 553-556, 2000. [PDF-Full text in Korean]

• Dong-Yup Lee, Hyungjin Park, Jehoon Song and Sunwon Park, "Environmental Purchasing for Green Supply Chain Management", Proc. '00 KIChE Fall meeting, Vol. 6, pp. 3033-3036, 2000. [PDF-Full text in Korean][presentation][presentation PDF-file]

• Jehoon Song, Hyungjin Park, Dong-Yup Lee and Sunwon Park, "Scheduling Model for Refinery Processes of Actual Size Considering Supply Chain Management", Proc. '01 KIChE Spring meeting, Vol. 7, pp. 565-568, 2001. [PDF-Full text in Korean]

• Young-gyun Oh, Heejin Lim, Dong-Yup Lee and Sunwon Park, "Life Cycle Assessment for Sulfuric and Nitric Acid Production Systems", Proc. '01 KIChE Spring meeting, Vol. 7, pp. 649-652, 2001.

• Mingu Kang, Heejin Lim, Dong-Yup Lee and Sunwon Park, "Life Cycle Assessment for Phosphoric Acid Production Systems", Proc. '01 KIChE Spring meeting, Vol. 7, pp. 653-656, 2001.

• Hodong Seo, Dong-Yup Lee, Sunwon Park, Liang-Tseng Fan, Shahram Shafie, Botond Bertók and Ferenc Friedler, "Graph-Theoretical Identification of Pathways for Biochemical Reactions", PRES'01, Florence, Italy, 2001. [PDF-Full text][presentation]

• Jehoon Song, Hyungjin Park, Dong-Yup Lee and Sunwon Park, "Scheduling Model for Actual Size Refinery Processes Considering Supply Chain Management", Proc. 2001 Korean Society of Supply Chain Management Spring meeting, 2001. [PDF-Full text in Korean]

• Dong-Yup Lee, Moonyong Lee, Yongho Lee and Sunwon Park, "Multiloop PID Controllers Tuning for Desired Closed Loop Responses", DYCOPS-6, Cheju Island, Korea, June 4 - 6, 2001. [PDF-Full text][presentation]

• Hodong Seo, Dong-Yup Lee, Sunwon Park, Liang-Tseng Fan, Shahram Shafie, Botond Bertók and Ferenc Friedler, "An Attempt for the Identification of Stoichiometrically Exact Mechanisms of the Conversion of Glucose to Pyruvate", Proc. '01 KIChE Fall meeting, Vol. 7, pp. 2953-2956, 2001. [PDF-Full text in Korean][presentation]

• Jehoon Song, Hyungjin Park, Dong-Yup Lee and Sunwon Park, "Scheduling for Actual Size Refinery Processes Considering Environmental Impacts with Multiobjective Optimization", Proc. '01 KIChE Fall meeting, Vol. 7, pp. 3007-3010, 2001. [PDF-Full text in Korean][presentation]

• Young-gyun Oh, Dong-Yup Lee and Sunwon Park, "Pathway Analysis and Dynamic Simulation of Metabolic Pathway in the Human Red Blood Cells", Proc. '02 KIChE Spring meeting, Vol. 8, pp. 357-360, 2002.

• Hongseok Yun, Dong-Yup Lee, Sang Yup Lee and Sunwon Park, "MetaFluxNetTM: a program for metabolic flux analysis (MFA)", International Conference on Control, Automation and Systems, 2002.

• Young-gyun Oh, Dong-Yup Lee and Sunwon Park, "Multiobjective Linear Programming in Flux Balance Analysis", Proc. '02 KIChE Fall meeting, Vol. 8, pp. 2997-3000, 2002.

• Dong-Yup Lee, Ralf Zimmer, Juliane Fluck, Daniel Hanisch and Sunwon Park, "Petri Net Representation and Simulation of Signal Transduction Network", Proc. '02 KIChE Fall meeting, Vol. 8, pp. 2857-2860, 2002.

• Dong-Yup Lee, Su Whan Sung, Sang Yup Lee and Sunwon Park, "Development of Pharmacokinetic/Pharmacodynamic Models Considering Physiological Delays and Uncertainties", AIChE Annual Meeting, Indianapolis, USA, November 3 - 8, 2002.

• Dong-Yup Lee, Sung Ho Yoon, Hongseok Yun, Sunwon Park and Sang Yup Lee, "Graph-Theoretical Identification of Multiple Flux Distiributions in Metabolic Flux Analysis", Korean Society for Bioinformatics annual meeting, Pusan, 2002.

• Dong-Yup Lee, Young-gyun Oh, Hongseok Yun, Sang Yup Lee and Sunwon Park, "Exploring Flux Distribution Profiles for Switching Pathways Using Multiobjective Flux Balance Analysis", 13th International Conference on Genome Informatics (GIW 2002), Tokyo, Japan, December 16 - 18, 2002.

• Dong-Yup Lee, Sungdeuk Moon, Paulo-Miguel Lopes and Sunwon Park, "Environmentally Friendly Scheduling of Primary Steelmaking Processes", Proc. Foundations of Computer-Aided Process Operations (FOCAPO 2003), pp. 355-358, Coral Springs, Florida, USA, January 12 - 15, 2003. [PDF-Full text][presentation]

• Young-gyun Oh, Hongseok Yun, Dong-Yup Lee, Sang Yup Lee and Sunwon Park, "Development of the Flux Balance Analysis Software Using Multiobjective linear programming", KIChE Spring meeting, Soonchun, 2003.

• Hongseok Yun, Dong-Yup Lee, Sang Yup Lee and Sunwon Park, "Development of a Software for Metabolic Flux Analysis (MFA)", KIChE Spring meeting, Soonchun, 2003.

• Sang Yup Lee, Dong-Yup Lee, Hongseok Yun and Sunwon Park, "MetaFluxNet: an Integrated Program for the Management of Metabolic Reaction Information and Quntitative Metabolic Flux Analysis", First International E.coli Alliance (IECA) Conference on Systems Biology of E.coli, Tsuruoka, Japan, June 23 - 25, 2003.

• Dong-Yup Lee, Hongseok Yun, Sang Yup Lee and Sunwon Park, "Comparative Metabolic Flux Analysis by MetaFluxNet", 11th International Conference on Intelligent Systems for Molecular Biology (ISMB 2003), Brisbane, Australia, June 29 - July 3, 2003.

• Young-gyun Oh, Dong-Yup Lee, Hongseok Yun, Sang Yup Lee and Sunwon Park, "Supporting SBML for representation and exchange of biochemical network models in MetaFluxNet", KIChE Fall meeting, Seoul, 2003.

• Soon Ho Hong, Dong-Yup Lee, Tae Yong Kim, Sang Yup Lee and Sunwon Park, "Effect of carbon substrate on the intracellular fluxes in succinic acid producing Escherichia coli", 2nd Annual Conference of the Korean Society for Bioinformatics (KSBI 2003), KAIST, Daejeon, October 31 - November 1, 2003.

• Dong-Yup Lee, Soon Ho Hong, Tae Yong Kim, Sang Yup Lee and Sunwon Park, "An Integrated Environment for Reconstruction and Analysis of Large-scale Metabolic Pathways", 2nd Annual Conference of the Korean Society for Bioinformatics (KSBI 2003), KAIST, Daejeon, October 31 - November 1, 2003.

• Young-Gyun Oh, Dong-Yup Lee, Sang Yup Lee and Sunwon Park, "Communication Between MetaFluxNet and Other Systems Biology Tools", 2nd Annual Conference of the Korean Society for Bioinformatics (KSBI 2003), KAIST, Daejeon, October 31 - November 1, 2003.

• Choamun Yun, Jinkuk Kim, Young-Gyun Oh, Dong-Yup Lee, Sunwon Park and Sang Yup Lee, "WebCell: a Web-based Dynamic Simulation Environment for Cellular Network Systems", 2nd Annual Conference of the Korean Society for Bioinformatics (KSBI 2003), KAIST, Daejeon, October 31 - November 1, 2003.

• Dong-Yup Lee, L. T. Fan, Sunwon Park, Sang Yup Lee, Shahram Shafie, Botond Bertók and Ferenc Friedler, "Synergistic Identification of Multiple Flux Distributions and Multiple Metabolic Pathways", AIChE Annual Meeting, San Francisco, USA, November 16 - 21, 2003.

• Sang Yup Lee, Dong-Yup Lee, Soon Ho Hong, Tae Yong Kim, Hongseok Yun, Young-Gyun Oh and Sunwon Park, "MetaFluxNet, a Program Package for Metabolic Pathway Construction and Analysis, and Its Use in Large-scale Metabolic Flux Analysis of Escherichia coli", 14th International Conference on Genome Informatics (GIW 2003), Yokohama, Japan, December 14 - 17, 2003.

Projects

• Project Manager, Korean National LCI Database Construction (National Infra-Industry/Basic Materials), KAIST, Ministry of Environment, 1999-2001.

• Development of Platform Technology for In Silico Physiomics: Development of Biotechnology Processes Based on Metabolic Network Modeling and Biological Reaction Simulation System, KAIST, Supported by IMT-2000 Project from the Ministry of Science & Technology and Ministry of Information & Communication, 2002-2004.

Research Interest

• Functional Genomics

• Proteomics

• Systems biology/biotechnology

• Metabolic and cellular engineering

Education

• B.S., Dept. of Biological Science, Youngnam Univ., Daegu, Korea (1994. 3. 1.-1998. 2. 28)

• M.S., Dept. of Chemical Engineering, Youngnam Univ., Daegu, Korea (1998. 3. 1.-2000. 2. 29)

• M. S. Thesis: Viability improvement of animal cells by genetic manipulation (Advisor: Prof. Moo Hwan Cho)

• Ph.D., Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, Korea (2000.3.1-2004.2.29) 

• Ph.D. Thesis: Proteome profiling and its application in developing recombinant Escherichia coli strains (Advisor: Prof. Sang Yup Lee)

• Post-doc., Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, Korea (2004.3-2004.7)

• Post-doc., Systems Biology Division, Proteomics Laboratory, The Wistar Institute, University of Pennsylvania, Philadelphia, PA, USA (2004.8-2006.6)

Honor/Awards

• A 96' National Transport Phenomena Meeting Awards (1996)

• Yeungnam University Scholarship from 1994 to 1998

• Election "an outstanding graduate of the KAIST"2004)

• Applied Biosystems Young Scientist Award (2004) in KHUPO 4th Annual International Proteomics Conference

Publications

•  4-1. Research Articles (Published in Peer-reviewed Journals)

• 1.  Han, M.-J., Yoon, S.S., and Lee, S.Y. "Proteome analysis of metabolically engineered Escherichia coli cells producing poly(3-hydroxybutyrate)" J. Bacteriol. 183(1):301-308 (2001).

• 2.  Yoon, S.H., Han, M.-J., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Combined transcriptome and proteome analysis of Escherichia coli during the high cell density culture" Biotechnol. Bioeng. 81(7):753-767 (2003). (Accelerated publication article)

• 3.  Han, M.-J., Jeong, K.J., Yoo, J.-S, and Lee. S.Y. "Engineering Escherichia coli for increased production of serine-rich proteins based on proteome profiling" Appl. Environ. Microbiol. 69(10): 5772-5781 (2003).

• 4.  Han, M.-J., and Lee, S.Y. "Proteome profiling and its use in metabolic and cellular engineering" Proteomics 3:2317-2324 (2003).

• 5.  Han, M.-J., Park, S.J., Park, T.J., and Lee, S.Y. "Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli" Biotechnol. Bioeng. 88(4): 426-436 (2004). (Cover article)

• 6.  Lee, S.H., Choi, J.-I., Han, M.-J., Choi, J.H., and Lee, S.Y. "Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its applications to enantioselective resolution in organic solvent" Biotechnol. Bioeng. 90(2):223-230 (2005).

• 7.  Park, S.J., Lee, S.Y., Cho, J., Kim, T.Y., Lee, J.W., Park, J.H., and Han, M.-J. "Global physiological understanding and metabolic engineering of microorganisms based on omics studies" Appl. Microbiol. Biotechnol. 23:1-13 (2005).

• 8.  Han, M.-J., Lee, J.W., and Lee, S.Y. "Enhanced Proteome Profiling by Inhibiting Proteolysis with Small Heat Shock Proteins" J. Proteome Res. 4:2429-2434 (2005).

• 9.  Han, M.-J., Park, S.J., Lee, J.W., Min, B.H., Lee, S.Y., Kim, S.-J., and Yoo, J.-S. "Analysis of poly(3-hydroxybutyrate) granule-associated proteome in recombinant Escherichia coli" J. Microbiol. Biotechnol. 16:901-910 (2006).

• 10. Han, M.-J., and Lee, S.Y. "The Escherichia coli Proteome: Past, Present, and Future Prospects" Microbiol. Mol. Biol. Rev. 70: 362-439 (2006).

• 11. Lee, Y.T., Han, M.-J., Lim, S.H., Park, S.H., Suh, H.S., Park, J.B., Kim, Y.I., and Lee, J.W. "Effect of Antibiotics on the Survival of Human Hepatocellular Carcinoma Cells under Hypoxic Conditions" J Korean surgical society 071(01): 31-38 (2006).

• 12. Han, M.-J., Herlyn, M., Fisher, A.B., and Speicher, D.W. "Microscale solution IEF combined with 2-D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts", Electrophoresis, 29(3):695-705 (2008).

• 13. Han, M.-J., Lee, J.W., Lee, S.Y. and Yoo, J.-S. "Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production", J. Biomedicine Biotechnol. 2008:1-12 (2008).

• 14. Xia, X.-X., Han, M.-J., Lee, J.W., Lee, S.Y. and Yoo, J.-S. "Comparison of the extracellular proteomes of Escherichia coli B and K-12 strains during high cell density cultivation", Proteomics 8:2089-2103 (2008).

• 15. Han, M.-J., Yun H., and Lee, S.Y. “Microbial small heat shock proteins and their use in biotechnology”, Biotechnol. Adv. in press (2008).

• 4-2. Books

• 1.  Lee, J.W, Han, M.-J., and Lee, S.Y., "Chapter 4. Improved 2-Dimensional Electrophoresis by Inhibiting Proteolysis with Small Heat Shock Proteins" Methods in Proteomics, The Korean Human Proteome Organization (KHUPO), 71-79 (2007)

• 2. Han, M.-J., and. Speicher D.W. "Microscale Isoelectric Focusing in Solution: A Method for More Comprehensive and Quantitative Proteome Analysis Using 1-D and 2-D DIGE Combined with MicroSol IEF Prefractionation", in Methods Molecular Biology: 2D PAGE: Sample Preparation and Fractionation (Posch, A., ed.), Humana, Totowa, NJ, 424:241-256 (2008)

• 3.  Han, M.-J. and Lee, S. Y.,  "Chapter 6.5, The Escherichia coli Proteome: Past, Present, and Future Prospects", In A. Böck, R. Curtiss III, J. B. Kaper, F. C. Neidhardt, T. Nyström, J. M. Slauch, and C. L. Squires (ed.), EcoSal—Escherichia coli and Salmonella: cellular and molecular biology. http://www.ecosal.org. ASM Press, Washington, D.C. (18 October 2007, posting date)

• 4. Jang, S.H., Han, M.-J., Lee, S.Y., Choi, J.H., Xia, X.-X. “Genome-wide technologies: DNA microarrays, phenotypic microarrays, and proteomics” in Metabolic Pathway Engineering Handbook, in press

• 4-3. Proceedings

• 1.  Han, M.-J., You, J.S., and Lee, S.Y. "Variation of proteome expression during the high cell density cultivation of Escherichia coli" Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 483-486 (2001).

• 2.  Yoon, S.H., Han, M.-J., Lim, K.B., and Lee, S.Y. "Combined analysis of transcriptome and proteome for high cell density cultivation of Escherichia coli" Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 845-848 (2001).

• 3.  Han, M.-J., Choi, J.H., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Proteome Analysis of Overproduction of Recombinant Protein in Escherichia coli by Fed-Batch Fermentations" Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 750-753 (2001).

• 4.  Han, M.-J., Choi, J.H., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Proteome Analysis of Overproduction of Human Leptin in Escherichia coli by Fed-Batch Fermentations" Theories and Applications of Chemical Engineering 7(2): 4075-4078 (2001).

• 5.  Lee, J.W., Han, M.-J., and Lee, S.Y. "Proteomic analysis of oleic acid stimulon in Escherichia coli and its application" Proceedings of the 17th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering 21 (2004).

• 6.  Han, M.-J., Lee, J.W., Lee, S.Y., and Yoo, J.-S. "Proteomic Signature of a Response to Long-Chain Fatty Acid in Escherichia coli and Its Application in Biotechnology" Frontiers in the Convergence of Bioscience and Information Technologies 2007, Ramada Plaza Jeju, Jeju-do, Oct 11-13 (2007).

• 4-4. Patents

• - 8 Applications of International Patent

•  - 6 Applications of Domestic Patent

Presentations

• 5-1. Proceedings

• 1.  Lee, S.Y., Yoon, S. H., Han, M.-J., and Jeong, K.J. "Combined analysis of transcriptome and proteome profiles during the high cell density cultivation of Escherichia coli" Biochemical Engineering XII, Sonoma, California, USA, June (2001).

• 2.  Han, M.-J., Yoon, S. H., and Lee, S.Y. "Transcriptome profile analysis of Escherichia coli during high cell density cultivation using DNA microarray" Biochemical Engineering XII, Sonoma, California, USA, June (2001).

• 3.  Lee, S.Y., Yoon, S.H., Han, M.-J., Yoo, J.-S., and Lim, G. "Transcriptome and proteome analysis of Escherichia coli during high cell density cultivation" The 9th International Symposium on Intelligent Systems for Molecular Biology, Copenhagen, Denmark, July (2001).

• 4.  Lee, S.Y. and Han, M.-J. "Analysis of serum proteome profiles in leukemia patients" Inaugural Symposium of AOHUOP & 2nd Annual Meeting of KHUPO, Seoul, Korea, March 26-27 (2002).

• 5.  Han, M.-J., Jeong, K.J., Yoo, J. -S., and Lee, S.Y. "Improvement of Recombinant Protein Productivity through Proteome Analysis" ChungNam-Kyushu International Symposium, Kumamoto, Japan, December, 6 (2002).

• 6.  Lee, S.Y., and Han, M. -J. "Proteome Profiling and Its use in Metabolic and Cellular Engineering" KHUPO, Seoul National University, Seoul, Korea, March 27-28(2003).

• 7.  Han, M.-J., Jeong, K.J., and Lee, S.Y. "Improvement of recombinant protein productivity through proteome analysis" Biochemical Engineering (XIII) 2003, Boulder, Colorado, USA, July 17-23 (2003)

• 8.  Choi, J.H., Han, M.-J., Jeong, K.J., and Lee, S.Y. "Using proteomics to engineer improved cell growth and increased production of heterologous proteins in Escherichia coli" APBioChEC '03, Carlton Crest Hotel, Brisbane, Australia, Nov 30 - Dec 4 (2003).

• 9.  Han, M.-J., Park, S.J., Lee, J.W., Yoo, J.-S., and Lee, S.Y. "Analyses of poly(3-hydroxybutyrate) granule-associated proteins in recombinant Escherichia coli" KHUPO 4th Annual International Proteomics Conference, March, 25-26 (2004).

• 10. Han, M.-J., Park, S.J., Park, T.J., Lee, J.W., and Lee, S.Y. "Essential roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli" KHUPO 4th Annual International Proteomics Conference, March, 25-26 (2004).-Poster award

• 11. Han, M.-J., Park, S.J. Park, T.J. Lee, D.Y. and Lee, S.Y. "Importance of small heat shock proteins in engineering recombinant protein production as revealed by proteome profiling" The 12th International Conference on Intelligent Systems for Molecular Biology (ISMB 2004), Glasgow, UK, July 31-August 4 (2004).

• 12. Lee, S.Y., Han, H.-J., Park, S.J., and Park, T.J. "Essential roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli" Metabolic Engineering V: Genome to Product, Lake Tohoe, California, USA, Sept. 19-23 (2004).

• 13. Park, J.P., Han, M.-J., Park, T.J., Lee, S.J., and Lee, S.Y. "Metabolic engineering of Escherichia coli for the production of polyamide" Metabolic Engineering V: Genome to Product, Lake Tohoe, California, USA, Sept. 19-23 (2004).

• 14. Lee, S.Y., Han, M.-J., Park, S.J, and Lee, J.W. "Roles and applications of small heat shock proteins for the production of recombinant proteins in Escherichia coli" 3rd Recombinant Protein Production Meeting, Tavira, Portugal, Nov 11-14 (2004).

• 15. Lee, J.W., Han, M.-J., Yoo, J.-S., and Lee, S.Y. "Proteomic analysis of Escherichia coli cultured in oleic acid and its application" KHUPO 5th International Proteomics Conference AOHUPO Workshop/Symposium, April 27-29 (2005).- Poster award

• 16. Han, M.-J., Park, S.J., Lee, J.W., Yoo, J.-S., and Lee, S.Y. "Analysis of poly(3-Hydroxybutyrate) granule-associated proteome in recombinant Escherichia coli" KHUPO 5th International Proteomics Conference AOHUPO Workshop/Symposium, April 27-29 (2005).

• 17. Speicher, D.W., Tang, H.-Y., Han M.-J., Wang, H., Ali-Khan, N., Echan, L.A., Tan, G., Musselman, I., and Joo, W.A. "The Multi-dimensional Approach to Cellular and Plasma Proteomics and Its Application to Human Cancers" The Association of Biomolecular Resource Facilities (ABRF) meeting, Long Beach, California, USA, Feb. 11-14 (2006).

• 18. Lee, J.W., Han, M-.J., and Lee, S.Y., "Improved proteome profiling method by inhibiting proteolysis with small heat shock proteins" The 3rd International E. coli Alliance Conference on Systems Biology 2006, Jeju, Korea (2006. 10.30-11.3).

• 19. Lee, J.W., Han, M.-J., Yoo, J.-S., and Lee, S.Y. "Mining Low Abundance Proteins by Inhibiting Proteolysis with Small Heat Shock Proteins" KHUPO 7th International Proteomics Conference Workshop, March 29-30 (2007).

• 20. Han, M.-J., Lee, J.W., Yoo, J.-S., and Lee, S.Y. "Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteins in two-dimensional gel electrophoresis" HUPO 6th Annual World Congress 2007, Coex, Seoul, Korea, Oct. 6-10 (2007).

• 21. Han, M.-J., Lee, J.W., Yoo, J.-S., and Lee, S.Y. "Proteomic signature of a response to long-chain fatty acid in Escherichia coli and its application in biotechnology" HUPO 6th Annual World Congress 2007, Coex, Seoul, Korea, Oct. 6-10 (2007).

• 22. Xia, X.-X., Han, M.-J., Lee, S.Y., and Yoo, J.-S. "Proteomic analysis of selectively released proteins from Escherichia coli B and K-12 strains grown in high cell density cultivation" HUPO 6th Annual World Congress 2007, Coex, Seoul, Korea, Oct. 6-10 (2007).

• 23. Han, M.-J., Echan, L.A., Fisher, A.B., and Speicher, D.W. "A method for comprehensive and quantitative proteome analysis using microscale solution IEF combined with 2-D DIGE" HUPO 6th Annual World Congress 2007, Coex, Seoul, Korea, Oct. 6-10 (2007).

• 24. Han, M.-J., Echan, L.A., Tang, H.-Y., Herlyn, M., and Speicher, D.W. "Identification of proteins and their pathways associated with increased metastatic potential of human melanoma cells using in depth three-dimensional protein profiling" HUPO 6th Annual World Congress 2007, Coex, Seoul, Korea, Oct. 6-10 (2007).

• 25. Han, M.-J., Lee, J.W., Lee, S.Y., and Yoo, J.-S. "Proteomic Signature of a Response to Long-Chain Fatty Acid in Escherichia coli and Its Application in Biotechnology" Frontiers in the Convergence of Bioscience and Information Technologies 2007, Ramada Plaza Jeju, Jeju-do, Oct 11-13 (2007).

• 5-2. Korean Conferences

• 1.  Han, M.-J., Cho, M.H., and Lee, J.W. "Antibiotics geneticin (G418) makes a hepatocellular cell line, HepG2, more resistant to hypoxia by efficient utilization of glucose" The Korean Society for Molecular Biology (1999).

• 2.  Han, M.-J., Cho, M.H., and Lee, J.W., "Improvement of metabolism under hypoxia by antibiotics geneticin (G418)" The Korean Society for Microbiology (1999).

• 3.  Han, M.-J., Yoon, S.S., and Lee, S.Y. "Proteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate)" Annual Meeting of The Korean Society for Applied Microbiology, Cheon-buk Univ., October (2000).

• 4.  Han, M.-J., You, J.S., and Lee, S.Y. "Variation of proteome expression during the high cell density cultivation of Escherichia coli" Annual meeting of Korean society for Biotechnology and Bioengineering, Konkuk Univ., Seoul, April (2001).

• 5.  Yoon, S.H., Han, M.-J., Jeong, K.J., Yoo, J.-S., Lim, B.K., and Lee, S.Y. "Transcriptome and proteome analysis of Escherichia coli during high cell density cultivation" Annual Meeting of Korean Society for Applied Microbiology, Chonan Sangnok Resort, June (2001).

• 6.  Han, M.-J., Choi, J.H., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Proteome Analysis of Overproducing Human Leptin System" The 14th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, Chungnam Univ., Daejeon (2001).

• 7.  Yoon, S.H., Han, M.-J., Lim, K.B., and Lee, S.Y. "Combined analysis of transcriptome and proteome for high cell density cultivation of Escherichia coli" Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, November (2001).

• 8.  Han, M.-J., Choi, J.H., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Proteome Analysis of Overproduction of Recombinant Protein in Escherichia coli by Fed-Batch Fermentations" Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, November (2001).

• 9.  Han, M.-J., Choi, J.H., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Proteome Analysis of Overproduction of Human Leptin in Escherichia coli by Fed-Batch Fermentations" KIChE Meeting, Daejeon (2001).

• 10. Lee, S.Y., Han, M.-J., and Yoon, S.S. "Proteome Analysis of Metabolically Engineered Escherichia coli Producing Poly (3-Hydroxybutyrate)" Korean Society of Medical Biochemistry and Molecular Biology, Seoul (2001).

• 11. Han, M .-J., Jeong, K. J., Yoo, J.-S., and Lee S Y. "Improvement of Recombinant Protein Productivity through Proteome Analysis" Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Chungju, October 12 (2002).

• 12. Han, M.-J., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Proteome analysis of recombinant Escherichia coli producing heterologous proteins" Korean Society for Bioinformatics Annual Meeting (KSBI 2002), Haeundae, Korea, November 15-16 (2002).

• 13. Han, M.-J., Lee, S.Y., Hong, S. H., and Park, S.J. "Role of Eda in polyhydroxyalkanoate biosynthesis deciphered from proteome analysis and metabolic flux analysis" Korean Society for Bioinformatics Annual Meeting (KSBI 2002), Haeundae, Busan, November, 15-16 (2002).

• 14. Han, M.-J., Jeong, K.J., Yoo, J.-S. and Lee,S.Y. "Rational design for improving cell growth and protein production of a recombinant Escherichia coli strain using a proteomic approach" Annual meeting of Korean Society for Biotechnology and Bioengineering Symposium, Busan, April 11-12 (2003).

• 15. Han, M.-J., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Using Proteomics to Engineer Improved Cell Growth and Increased Production of Serine-Rich Heterologous Proteins in Escherichia coli." Annual meeting of Korean Society for Microbiology and Biotechnology Symposium (KMB 2003), Muju, June 24-26 (2003).

• 16. Han, M.-J., Jeong, K.J., Yoo, J.-S., and Lee, S.Y. "Engineering Escherichia coli for Increased Productivity of Serine-Rich Proteins Based on Proteome Profiling" Annual meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, October 22-24 (2003).

• 17. Bang, H.O., Han, M.-J., Park, S.J., and Lee, S.Y. "Roles of the Escherichia coli small heat shock proteins IbpA and IbpB in recombinant proteins productionin recombinant proteins production" Annual fall meeting of KIChE, Seoul, October 24-25 (2003).

• 18. Han, M.-J., and Lee, S.Y. "A Proteomic Approach for Engineer Improved Cell Growth and Increased Productivity of Serine-Rich Protein" KSBI 2003, KAIST, Daejeon, October 31-November 1 (2003).

• 19. Han, M.-J., Park, S.J., Park, T.J., and Lee, S.Y. "Essential roles and applications of the Escherichia coli small heat shock proteins IbpA and IbpB in the production of recombinant proteins" Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Daegu, April 16-17 (2004).

• 20. Han, M.-J. "Microbial proteomics: Enhanced recombinant protein production by E. coli engineered based on proteome profiling" 2004 International Meeting of the Microbiological Society of Korea, Sangnok Resort Convention Center, Cheonan, May 13-14 (2004).

• 21. Han, M.-J., Park, S.J., Lee, J.W., Yoo, J.-S., and Lee, S.Y. "Proteome Analysis of Poly(3-Hydroxybutyrate) Granule-Associated Proteins in Recombinant Escherichia coli" Annual Meeting of Korean Society for Microbiology and Biotechnology Symposium, Deagu, June 21-23 (2004).

• 22. Han, M.-J., Park, S.J., Lee, J.W., Park, T.J., and Lee, S.Y. "Functions of small Heat Shock Proteins in the Production of Recombinant Proteins in Escherichia coli and its Applications" Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Cheongju, October 13-15 (2004).

• 23. Lee, J.W., Han, M.-J., and Lee, S.Y. "Proteomic analysis of oleic acid stimulon in Escherichia coli and its application" The 17th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, December 4-5 (2004).

• 24. Han, M.-J., Park, S.J., Lee, J.W. and Lee, S.Y. "Use of small heat shock proteins in the production of recombinant proteins in Escherichia coli and its applications" Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, April 15-16 (2005).

• 25. Lee, J.W., Han, M.-J., Yoo, J.-S., and Lee, S.Y. "Proteomic analysis of Escherichia coli cultured in oleic acid and its application" Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, April 15-16 (2005).

• 26. Lee, J.W., Han, M-.J., Yoo, J.-S. and Lee, S.Y. "Proteomic signature of a response to long chain fatty acid in Escherichia coli and its application" The 62th Korean Society for Biochemistry and Molecular Biology (KSBMB) annual meeting, Seoul, May 19-20 (2005).

• 27. Lee, J.W., Han, M.-J., Yoo, J.-S., and Lee, S.Y. "Proteomic Signature of a Response to Long Chain Fatty Acid in Escherichia coli and Its Application" Annual Meeting & International Symposium of the Korea Society for Microbiology and Biotechnology (KMB), June 30 - July 1 (2005).

• 28. Han, M.-J., and Lee, S.Y., "Metabolic Engineering for Strain Improvement Based on Proteome Profiling" 2006 Annual Fall Meeting of Korean Society for Biotechnology and Bioengineering Symposium, September 7-8 (2006)

• 29. Lee, J.W., Han, M-.J., and Lee, S.Y., "Improved Proteome Profiling Method Using Small Heat Shock Proteins by Inhibiting Proteolysis" 2006 International Meeting of the Federation of Korean Microbiological Societies, October 19-20 (2006)

• 30. Han, M.-J., Lee, J.W., and Lee, S.Y., "General improvement of proteome profiling by inhibiting proteolysis with small heat shock proteins" Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Incheon, April 27 (2007)

• 31. Han, M.-J., Lee, J.W., Lee, S.Y., Yoo, J.S., "Proteomic response to a long-chain fatty acid in Escherichia coli and its application in the production of recombinant proteins" The Korean Society for Microbiology and Biotechnology, COEX Grand Ballroom, Seoul, June 28-29 (2007)

• 5-3. Invited Talk

• 1.  Han, M.-J. "The Escherichia coli Proteome: Past, Present, and Future Prospects" Symposium on Pathogen & Proteome, Korea Centers for Disease Control and Prevention, National Institute of Health, July 28 (2006).

• 2.  Han, M.-J. "Systems-level analysis for the metabolic engineering of microorganism by proteome analysis"Korean Society for Mass Spectrometry, Phoenix Park, Gangwon-do, Aug 23-25 (2006).

• 3.  Han, M.-J. "Metabolic Engineering for Strain Improvement Based on Proteome Profiling"The Korean Society for Biotechnology and Bioengineering, COEX, Seoul, Sept 7-8 (2006).

• 4.  Han, M.-J. "프로테오믹스 (Proteomics)의 이해와 생명과학 연구에서의 활용" University of Yeungnam University, School of Display & Chemical Engineering, an autumn seminar 2006, Sept 15 (2006).

• 5.  Han, M.-J. "The Multi-dimensional Approach to Cellular and Plasma Proteomics and Its Application to Human Cancers"Kyungpook National University, School of Medicine, BK21 Program Seminar, Sept 19 (2006).

• 6.  Han, M.-J. "The Escherichia coli Proteome: Past, Present, and Future Prospects" Department of Biotechnology, Daegu University, Nov 16 (2006).

Research Interest

• Biocatalysts

• Kinetic resolution

• Enantiomerically pure compound

Education

• 1998 (B.S.) Dept. of Biological Science, KAIST, Taejon, Korea

• 2000 (M.S.) Dept. of Biological Science, KAIST, Taejon, Korea

• 2004. 8(Ph. D)  Department of Chemical Engineering, KAIST, Taejon, Korea

Sissertation title

• M.S. Thesis : Development of fed-batch culture for the production of Medium-chain-length Polyhydroxyalkanoate by Pseudomonas (Advisor: Professor Sang Yup Lee)

• Ph.D. Thesis : Synthesis of enantiomerically pure compounds by biocatalysis (Advisor: Professor Sang Yup Lee)

Publications

•  International Journals

• 1. Lee, S.H., Oh, D.H., Ahn, W.S., Lee, Y., Choi, J., and Lee, S.Y. , “Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high cell density cultivation of Aeromonas hydrophila”, Biotechnol. Bioeng. 67:240-244(2000)

• 2.  Lee, S.Y. , Choi, J., and Lee, S.H. “Production of polyhydroxyalkanoates by fermentation of bacteria”Macromol. Symp. 159:259-266(2000)

• 3.  Lee, S.Y. , Wong, H.H., Choi, J., Lee, S.H., Lee, S.C., and Han, C.S., “Production of medium-chain-length  polyhydroxyalkanoates by high cell density cultivation of  Peudomonas putida under phosphorus limitation”, Biotechnol. Bioeng., 68:466-470(2000)

• 4. Park, S.H., Lee, S.H., and Lee, S.Y., "Preparation of optically active beta-amino acids from microbial polyester polyhydroxyalkanoates", J. Chem. Reseach (S), 498-499(2001)

• 5. Lee, S.Y., Hong, S.H., Lee, S.H., Park, S.J. "Fermentative Production of Chemicals That Can Be Used for Polymer Synthesis", Macromolecular Bioscience, 4 : 157-164 (2004).

• 6. Lee, S. H., Choi, J., Park, S. J., Lee, S.Y., Park, B. C. " Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and its use in enantioselective biocatalysis", Appl. Environ. Microbiol. 70(9) : 5074-5080 (2004).

• 7. Lee, S. H., Choi, J.H., Park, S.H., Choi, J.-I., and Lee, S.Y., "Enantioselective resolution of racemic compounds by cell surface displayed lipase", Enzyme Microbial Technol., 35 : 429-436 (2004).

• 8. Lee, S.H., Choi, J.-I., Han, M.-J., Choi, J.H., and Lee, S.Y. "Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its applications to enantioselective resolution in organic solvent", Biotechnol. Bioeng. in press(2004).

• Korean Journals

• 1. Lee, Y., Lee, S.H., Lee, S.Y.  “Fed-batch culture of Aeromonass hydrophila for the production of poly(3-hydroxybutyrate-co-hydroxyhexanoate) using two carbon sources”, Biotechnol.Bioproc. Eng. 4:195-198(1999)

• 2.  Choi, J.-I., Lee, S.H., and Lee, S.Y., "Isolation and characteristics of polyhydroxyalkanoates producing Pseudomonas sp. MBEL21", Kor. J. Microbiol. Biotechnol. 32(2) : 123-127 (2004).

• Korean Proceedings

• 1. Lee, S.H., Choi, J, and Lee, S.Y.  “Economical recovery of poly(3-hydroxybutyrate)from recombinant Escherichia coli by simple digestion” Proceedings of The 12th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, 331-332 (1999)

• 2. Lee, S.H., and Lee, S.Y.  “Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high cell density cultivation of Aeromonas Hydrophila” Proceedings of The 12th Taejon/Chungnam-Kyushu Symposium on Chemical Engineering, 325-326 (1999)

• 3. Lee, S.H., and Lee, S.Y.  “Production of medium-chain-length polyhydroxyalkanoates by the fed-batch culture of Pseudomonas putida” Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 329-330 (1999) Kangwon University.

• 4. Lee, S.H., Park, S.J., Lee, S.Y., and Park, S.H.  “ Production of Medium-chain-length Polyhydroxyalkanoate from Newly Screened Pseudomonas sp. MBEL21”, Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 153-156 (2001, Apr.)

• 5. Choi, J.H., Lee, S.H., Park, S.H. and Lee, S.Y. “Chiral Resolution by Lipase on the Eshcherichia coli Cell Surface”, Theories and Applications of Chemical Engineering 7(2): 4027-4030 (2001).

• International Conferences

• 1. Lee, S.Y., Lee, Y., Park, S., Lee, S.H., “Production of (R)-hydroxycarboxylic acids by bacteria”, ISBP/BEDPS 2000, Honolulu, Hawaii, December (2000)

• 2. Park, S.H., Lee, S.H., Lee, Y., Lee, S.Y., “Preparation of optically active β-amino acids from microbial polymer”, 221st National meeting of American Chemical Society”, San Diego,CA, USA, April (2001)

• 3. Lee, S. H., Choi, J.H., and Lee, S.Y., "Resolution of racemic compounds by genetically immobilized lipase", Metabolic Engineering IV applied Systems Biology, Castelvecchio Pascoli, Italy, October 6-11(2002) 

• 4. Lee, S. H., Park, J.P., Park, S.J., and Lee, S.Y., "Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates", Metabolic Engineering IV applied Systems Biology, Castelvecchio Pascoli, Italy, October 6-11(2002) 

• 5. Choi, J.H., Lee, S.H., and Lee, S.Y., "Display of polyhistidine and lipase on the surface of Escherichia coli by using the salmonella ompC as an anchoring motif and its application to chemoenzymatic synthesis", Biochemical Engineering (XIII) 2003, Boulder, Colorado, USA July 17-23 (2003) 

• 6. Lee, S.H., Choi, J.H., and Lee, S.Y., "Dynamic kinetic resolution using cell surface displayed biocatalysts", Biochemical Engineering (XIII) 2003, Boulder, Colorado, USA July 17-23 (2003) 

• 7. Choi, J.H., Lee, S.H., and Lee, S.Y., "Production of chiral compounds by cell surface displayed lipase", APBioChEC '03, Carlton Crest Hotel, Brisbane, Australia, 30 Nov - 4 December (2003) 

• Korean Conferences

• 1. Lee, S.H., and Lee, S.Y. “Production of medium-chain-length polyhydroxyalkanoates by the fed-batch culture of Pseudomonas putida” Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 329-330 (1999) Kangwon University. 

• 2. Lee, S.H. and Lee, S.Y., "Production of Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) by high cell density cultivation of Aeromonas Hydrophila", The 12th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, Kongju Univ., Kongju (1999) 

• 3. Lee, S.H., Choi, J., and Lee, S.Y., "Economical recovery of poly (3-hydroxybutyrate) from recombinant Escherichia coli by simple digestion", The 12th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, Kongju Univ., Kongju (1999) 

• 4. Lee, S.H., Park, S.J., Lee, S.Y., Park, S.H. “ Production of Medium-chain-length Polyhydroxyalkanoate from Newly Screened Pseudomonas sp. MBEL21”, Proceedings of Korean society for Biotechnology and Bioengineering Symposium, 153-156 (2001, Apr.)

• 5. Choi, J.H., Lee, S.H., Park, S.H. and Lee, S.Y. “Chiral Resolution by Lipase on the Eshcherichia coli Cell Surface”, Theories and Applications of Chemical Engineering 7(2): 4027-4030 (2001).

• 6. Lee, S.H., Choi, J.H., and Lee, S.Y., "Production of chiral compounds by cell surface displayed lipase", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Busan, Korea, April 11-12(2003) 

• 7. Lee, S.H., Choi, J.H., and Lee, S.Y., "Kinetic resolution of beta-lactams by cell surface displayed Lipase", Annual Spring Meeting of KIChE, Soonchun, April 25-26(2003) 

• 8. Lee, S.H., and Lee, S.Y., "Dynamic kinetic resolution of styrene oxide by metabolically engineered Escherichia coli", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, Korea, October 22-24(2003) 

• 9. Lee, S.H., and Lee, S.Y., "Kinetic resolution of epoxides by metabolically engineered Escherichia coli", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Seoul, Korea, October 22-24(2003) 

• 10. Lee, S.H. and Lee, S.Y., "Cell surface display of lipase using Escherichia coli FadL as an anchoring motif", Annual Meeting of Korean Society for Biotechnology and Bioengineering Symposium, Daegu, Korea, April 16-17(2004)

• Book chapter

• 1.  Lee, S. Y., Choi, J., Lee, S. H., “Production of polyhydroxyalkanoates by fermentation of bacteria”, pp. 259-266 In: Polymer Science and Industrial Research in the Fast-changing Age (Kajiyama, T. ed) Wiley-VCH, Germany (2000)

• 2.  Lee S.Y., Park S.H., Lee Y., Lee S.H., "Production of chiral and other valuable compounds from microbial polyesters", Biopolymers Vol 4, Ch.14.pp.375-388, VCH-Wiley, Weinheim, Germany (2001)

• 3.  Lee, S.Y., Park, S.H., Hong, S.H., Lee, Y., Lee, S.H., "Fermentative Production of Building Blocks for Chemical Synthesis of Polyesters", Biopolymers Vol 3b, Ch 10, pp.265-326, VCH-Wiley, Weinheim, Germany (2001)

• 4.  Lee, S.Y., Park, S.J., Park, J.P., Lee, Y., Lee, S.H., "Economic aspects of biopolymer production", Biopolymers Vol.10,Ch.10, pp.307-337, VCH-Wiley, Weinheim Germany (2003)

• 5.  Lee, S.Y., Park, S.J., Lee, Y., Lee, S.H., "Biotechnological process for production of monomers for subsequent chemical polymer synthesis", Biopolymers Vol.10,Ch.9, pp.281-305, VCH-Wiley, Weinheim, Germany (2003)

Research Interest

• NANOBIOTECHNOLOGY

• Cell, Protein Patterning and Surface Modification

• Protein Electrochemistry

• Biosensor/Biochip

• Functionalization of Carbon Nanotube

• Synthesis of Nanoparticle/Nanowire/Nanocarrier

• Biofuel Cell

• BIOCATALYSIS AND BIOTRANSFORMATION

• PROTEIN CHEMISTRY AND ENGINEERING

• TISSUE ENGINEERING

Education

• 1991.3~1998.3 Department of Biotechnology, Daegu University (BS), B.S. in Biotechnology, February 1998, Advisor: Professor Jong Won Yun

• 1998.3~ 2000.2 MS in Department of Biotechnology, Daegu University (MS), M.S. in Biotechnology, Advisor: Professor Jong Won Yun, Thesis: Production of fructooligosaccharides (FOS) from a novel transfructosylating enzyme from Bacillus sp.

• 2001.9 ~2004.8 Department of Chemical Engineering, KAIST, Ph. D course, Ph.D. in Chemical & Biomolecular Engineering, Advisor: Professor Sang Yup Lee, Thesis: Development of nanobiosensors for biotechnological and environmental applications

Additional Experience

• REPUBLIC OF KOREA Marine Corps (ROKMC), Kimpo, Korea

Corporal, June 10, 1992- September 10, 1994

Awards

• The 1st Excellent R&D Award, Graduate School of Daegu University (2000)

• Best Paper in Poster Section, KSBB (2005)

Publications

•  1)Papers:

• M.S. course (March 1, 1998~Fubruary, 2001):

• 1. Park, J.P., Kim, D.H., Kim, D.S., and Yun, J.W.: Enzymatic production of inulo-oligosaccharides from chicory juice. Biotechnol Lett, 20(4) 385~388 (1998)

• 2. Park JP, Bae JT, and Yun JW : Critical effect of ammonium ions on the enzymatic reaction of a novel transfructosylating enzyme for fructooligosaccharide formation from sucrose. Biotechnol Lett, 21(10) (1999) 

• 3. Park JP, Bae JT, You DJ, Kim BW and Yun JW: Production of inulooligosaccharides from inulin by a novel endoinulinase from Xanthomonas sp. Biotechnol Lett, 21(12) (1999)

• 4. Yun JW, Park JP, Song CH, Lee CY, Kim JH, and Song SK : Continuous production of inulo-oligosaccharides from chicory juice by immobilized endoinulinase. Bioprocess Eng, 22(3) 189~194 (2000)

• 5. Kim BW, Kwon HJ, Park YH, Nam SW, Park JP and Yun JW : Production of a novel transfructosylating enzyme from Bacillus macerans EG-6. Bioprocess Eng, 23(1) 11~16 (2000)

• 6. JT Bae, J Sinha, JP Park, CH Song, and JW Yun : Optimization of submerged culture conditions for exo-biopolymer production by Paecilomyces japonica. J Microbiol Biotechnol, 10 (4) 482~487 (2000)

• 7. Kim YM, Park JP, Sinha J, Lim KH, Yun JW : Acceptor reaction of a novel transfructosylating enzyme from Bacillus sp.. Biotechnol Lett, 23(1) 13~16 (2001)

• 8. Cho YJ, Sinha J, Park JP, Yun JW : Production of inulooligosaccharides from chicory extract by endoinulinase from Xanthomonas oryzae No.5. Enzyme Microb Technol, 28(4-5), 439~445 (2001)

• 9. JT Bae, JP Park, J Sinha, CH Song and JW Yun: Effect of carbon source on the mycelial growth and exo-biopolymer production by submerged culture of Paecilomyces japonica. J Biosci Bioeng, 91(5) 522~524 (2001)

• 10. Park JP, Yun JW : Utilization of chicory roots for microbial endoinulinase production. Lett Appl Microbiol, 33(3) 183~187 (2001) 

• 11. JP Park, TG Oh, JW Yun : Purification and characterization of a novel fructosyltransferase by Bacillus macerans EG-6. Process Biochem, 37, 471~476 (2001) 

• 12. Park JP, SW Kim, HJ Hwang and JW Yun :Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps millitaris. Lett Appl Microbiol, 32, 1~6 (2001)

• 13. J. Sinha, JT Bae, JP Park, KH Kim, CH Song, and JW Yun: Changes in morphology of Paecilomyces japonica and its effect on broth rheology during production of exo-biopolymer. Appl Microbiol Biotechnol, 56, 88~92 (2001)

• 14. YJ Cho, J. Sinha, JP Park, JW Yun: Production of inulooligosaccharides from a dual endoinulinase system of Xanthomonas sp. and Pseudomonas sp. Enzyme Microb Technol, 29, 428~433 (2001)

• 15. J Sinha, JT Bae, JP Park, JW Yun: Effect of substrate concentration on broth rheology and fungal morphology during exo-biopolymer production by Paecilomyces japonica in a bioreactor. Enzyme Microb Technol, 29, 392~399 (2001)

• 16. JP Park, CH Song, JW Yun : Stimulatory effect of plant oils and fatty acids on the exo-biopolymer production in Cordyceps militaris. Enzyme Microb Technol. 31, 250-255 (2002)

• 17. JP Park, YM Kim, SW Kim, HJ Hwang, YJ Cho, YS Lee, CH Song, JW Yun : Effect of agitation intensity on the exo-biopolymer production and mycelial morphology in Cordyceps militaris. Lett Appl Microbiol. 34(6), 433~438 (2002)

• 18. YJ Cho, JP Park, HJ Hwang, SW Kim, JW Choi, JW Yun: Production of red pigment by submerged culture of Paecilomyces sinclairii. Lett Appl Microbiol. 35(3) , 195-202 (2002) 

• 19. SW Kim, HJ Hwang, JP Park, YJ Cho, CH Song,, JW Yun : Mycelial growth and exo-biopolymer production by submerged culture of various edible mushrooms under different media. Lett Appl Microbiol. 34(1) 56~61 (2002)

• 20. JP Park, YM Kim, SW Kim, HJ Hwang, YJ Cho, YS Lee, CH Song, JW Yun : Effect of aeration rate on the mycelial morphology and exo-biopolymer production in Cordyceps militaris. Process Biochem. 37, 1257~1262 (2002).

• Ph.D course (September 1, 2001~)

• 21. Park SJ, Park JP, Lee SY : Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermentor. Biotechnol Lett 24(3), 185-189 (2002)

• 22. Park, S.J, Park, J.P, Lee, S.Y. " Metabolic engineering of Escherichia coli for the production of medium -chain-length polyhydroxyalkaroates rich in specific monomers", FEMS Microbiol. Lett. 214:217-222 (2002)

• 23. Park, S.J., Park, J.P., Lee, S.Y., and Doi, Y., "Enrichment of specific monomer in medium-chain-length poly (3-hydroxyalkanoates) by amplification of fadD and fadE genes in recombinant Escherichia coli ", Enzyme Microbial Technol., 33 : 62-70 (2003)

• 24. Hong SH, Park S J, Moon S Y, Park J P and Lee SY . In Silico prediction of the importance of Enter-Doudoroff pathway in poly(3-hydroxybutyrate) production. Biotechnol Bioeng. 83(7) : 854-863 (2003)

• 25. Jong Pil Park, Seok Jae Lee, Tae Jung Park, Kyung-Bok Lee, Insung S. Choi, Sang Yup Lee, Min-Gon Kim and Bong Hyun Chung. Microcontact printing of biotin for selective immobilization of streptavidin-fused proteins and SPR analysis. BBE 9:137-142 (2004)

• 26. Park, T.J., Lee, K.-B., Lee, S.J., Park, J.P., Lee, Z.-W., Lee, S.Y., and Choi, I.S. "Micropatterns of spores displaying heterologous proteins", J. Am. Chem. Soc., 126(34):10512-10513 (2004).

• 27. Jong Pil Park, Seok Jae Lee, Tae Jung Park, Kyung-Bok Lee, Insung S. Choi, Min Gon Kim, Bong Hyun Chung, Zee-Won Lee, and Sang Yup Lee: Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner. Biotechnol. Bioeng, In Press (2005)

• 28. S.J. Lee, J.P. Park, T. J. Park. S.N. Lee, J.K. Park, S.Y. Lee. Visual detection of specific protein-protein interaction via PHB microsphere using substrate binding domain. in preparation , Anal. Chem, being revised (2005)

•      2) Book chapter:

• 1. Lee, S.Y., Park, S.J., Park, J.P., Lee, Y. and Lee, S.H. "Economic aspects of biopolymer production", Biopolymers Vol.10, Ch.10, pp.307-337, VCH-Wiley, Weinheim Germany (2003)

• 2. Polyhydroxyalkanoates in nanobiotechnology: application to protein-protein interaction studies, Horizon Scientific Prss, Submitted (2005).

Presentation

•  1) Domestic Conference

• M.S. Course (March 1, 1998 ~ February, 2001) :

• 1. JP Park, DH Kim and JW Yun : Inulo-oligosaccharide production using chicory juice from Pseudomonas sp. Annual Meeting of the Korean Society for Biotechnology and Bioengineering, (1998.4)

• 2. JP Park, JW Yun : Enzymatic production of inulo-oligosaccharide from chicory juice by endoinulinase immobilized on carrier materials. Annual Meeting of the Korean Society for Biotechnology and Bioengineering, (1998.10)

• 3. JH Kim, JP Park, JW Yun and SK Song : Continuous production of inulooligosaccharides from chicory juice by immobilized endoinulinase. Annual Meeting of the Korean Society for Biotechnology and Bioengineering,(1999.4)

• 4. JP Park, BC Oh, TK Oh and JW Yun : Purification and characterization of a novel transfructosylating enzyme from Bacillus sp. Annual Meeting of the Korean Society for Applied Microbiology, (1999.4)

• 5. JP Park, JW Yun : Production of inulooligosaccharides from inulin by Xanthomonas sp." Annual Meeting of the Korean Society for Applied Microbiology, (1999. 10).

• 6. JP Park, JT Bae, JW Yun : Critical effects of ammonium ions on the enzymatic reaction of a novel transfructosylating enzyme for fructooligosaccharide production from sucrose. Annual Meeting of the Korean Society for Applied Microbiology (1999.10)

• 7. JT Bae, J Sinha, JP Park and JW Yun : Optimization of culture conditions for exo-biopolymer production by Paecilomyces japonica. Annual Meeting of the Korean Society for Biotechnology and Bioengineering (2000.4)

• 8. JP Park, J Sinha and JW Yun : Utilization of chicory roots as a carbon source for endoinulinase production in Xanthomonas sp. Annual Meeting of the Korean Society for Applied Microbiology, (2000.4)

• 9. JP Park, JT Bae and JW Yun : Inulo-oligosaccharide production using chicory juice from Xanthomonas sp. Annual Meeting of the Korean Society for Applied Microbiology, (2000.4)

• 10. YJ Cho, JP Park, J Sinha and JW Yun : Inulo-oligosaccharide production using chicory juice from Xanthomonas sp. Annual Meeting of the Korean Society of Food Science and Technology (2000.5)